Evaluation of sperm motility with CASA-Mot: which factors may influence our measurements?
Marc Yeste A C , Sergi Bonet A , Joan E. Rodríguez-Gil B and Maria M. Rivera Del Álamo B
+ Author Affiliations
- Author Affiliations
A Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Institute of Food and Agricultural Technology, Faculty of Sciences, University of Girona, C/ Maria Aurèlia Campany 69, Campus Montilivi, E-17003 Girona, Spain.
B Unit of Animal Reproduction, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Building V, Campus Bellaterra s/n, E-08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain.
C Corresponding author. Email: marc.yeste@udg.edu
Reproduction, Fertility and Development 30(6) 789-798 https://doi.org/10.1071/RD17475
Submitted: 7 November 2017 Accepted: 13 January 2018 Published: 14 March 2018
Abstract
Computer-aided sperm analysis (CASA) is now routinely used in IVF clinics, animal breeding centres and research laboratories. Although CASA provides a more objective way to evaluate sperm parameters, a significant number of factors can affect these measurements. This paper classifies these factors into four categories: (1) sample and slide (e.g. preincubation time, type of specimen and type of chamber slide); (2) microscope (e.g. light source and microscope stage); (3) hardware and software, including the settings of each system; and (4) user-related factors. We review the effects of the different factors in each category on the measurements made and emphasise the need to take measures to standardise evaluations. The take-home message of the present article is that there are several commercial and useful CASA systems, and all are appropriate for routine analysis. Non-commercial systems may also be good choices when the user needs to adapt the device to specific experimental conditions. In both cases (commercial and non-commercial), it is important that standard protocols are put in place for evaluation, as well as methods to validate the system.
Additional keywords: animal, computer-aided sperm analysis (CASA), human, spermatozoa.
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