Physiology and gene expression of the rice landrace Horkuch under salt stress
Laisa A. Lisa A D , Sabrina M. Elias A D , M. Sazzadur Rahman A B , Saima Shahid A , Tetsushi Iwasaki C , A. K. M. Mahbub Hasan A , Keiko Kosuge C , Yasuo Fukami C and Zeba I. Seraj A D E
+ Author Affiliations
- Author Affiliations
A Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh.
B Plant Physiology Division, Bangladesh Rice Research Institute, Gazipur-1701, Bangladesh.
C Research Center for Environmental Genomics, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
D These authors contributed equally to the work of this manuscript.
E Corresponding author. Email: zebai@univdhaka.edu
Functional Plant Biology 38(4) 282-292 https://doi.org/10.1071/FP10198
Submitted: 5 October 2010 Accepted: 23 February 2011 Published: 8 April 2011
Abstract
Good donors in breeding for salt tolerance are a prerequisite for food security under changing climatic conditions. Horkuch, a farmer-popular salt tolerant rice (Oryza sativa L.) variety from the south-west coast of Bangladesh was characterised up to maturity under NaCl stress, together with a modern variety (BRRI dhan41), a sensitive control (BRRI dhan29) and Pokkali, the salt-tolerant benchmark for rice. Horkuch had low reduction in shoot biomass, a low Na : K ratio in flag leaves, a low percent reduction in yield and good partitioning of Na in the older leaves, and maintained high levels of Ca and Mg in the flag leaves. In order to understand the physiology at the molecular level, the expression of salt-responsive genes was investigated using microarray analysis. Salt-stressed cDNA of Horkuch seedlings were hybridised with cDNA probes synthesised mainly from database sequences of Arabidopsis thaliana (L.) Heynh. The upregulated genes included transcription factors, signal transducers, metabolic enzymes, reactive oxygen species (ROS) scavengers, osmoprotectants and some specific salt-induced transcripts. An increase in expression of photosynthesis-related genes as well ROS scavengers suggested that this could be the reason for the better yield performance of Horkuch. The data therefore indicate Horkuch as a potential donor alternative to Pokkali in breeding programs for salt tolerance.
Additional keywords: Bangladesh, donor, gene expression, Oryza sativa L., salt response.
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