Functional Plant Biology
Volume 44 Number 6 2017
FP16164Use of multicolour fluorescence imaging for diagnosis of bacterial and fungal infection on zucchini by implementing machine learning
Remote sensing has become an essential tool in phenotyping and precision agriculture to minimise the impact of cultural management on environment and human health. Several imaging techniques are currently in use for the detection of plant stress, but the information generated requires of increasingly advanced mathematical tools. Multicolour fluorescence imaging appears as a promising tool as data provider to feed mathematical models classifying healthy and infected zucchini plants.
FP16362Photosynthetic acclimation to elevated CO2 concentration in a sweet pepper (Capsicum annuum) crop under Mediterranean greenhouse conditions: influence of the nitrogen source and salinity
An increased CO2 concentration in the atmosphere improves yield of the horticultural crops but its efficiency is reduced after long-term exposure. We studied the evolution of the sweet pepper crop response to CO2 enrichment in Mediterranean greenhouse conditions under different salinity levels. The study revealed the crop phase when high CO2 concentration acclimation occurs and, thus, the point when removal of the CO2 supply could improve its efficiency.
Winter and spring are crucial periods for long-lived woody species of temperate regions because of the considerable impact on the plants’ water relations. In this study on high-yield apple cultivars, we show which climatic and species-specific factors are primarily responsible for the impairment of the water transport system in winter as well as its restoration in spring. Our findings contribute to the general knowledge about tree hydraulics but are also of relevance for fruit growers.
FP16285On the induction of injury in tomato under continuous light: circadian asynchrony as the main triggering factor
Unlike other species, tomato plants need to ‘sleep’ 8 h a day to remain healthy; that is, continuous light injures tomatoes. We experimentally tested many proposed explanations for this enigma and discarded all but one, which suggest that the presence of a light – normally a ‘day’ signal – when the internal circadian clock indicates ‘it’s night-time’ is central to this disorder: we call this conflict ‘circadian asynchrony’. These findings help in understanding the complex co-ordination between metabolism, light and time.
FP16365Morpho-physiological responses to dehydration stress of perennial ryegrass and tall fescue genotypes
Climate change will shift the adaptive regions of most forage grasses. We investigated the responses of two tall fescue genotypes and one perennial ryegrass to increasing drought and found that at the physiological and metabolic levels drought was imposed more slowly in tall fescue. Tall fescue may fit into future demands for forage grasses due to its good drought resistance.
FP16300Evaluation and application of a targeted SPE-LC-MS method for quantifying plant hormones and phenolics in Arabidopsis
The quantitation of plant hormones by LC-MS remains challenging because they belong to different chemical classes with contrasted analytical imperatives, and matrix effects often compromise reliable measurements. Here we present a technique based on accurate-mass LC-MS taking advantage of matrix elimination by solid phase extraction before analysis. This method is applied to Arabidopsis samples and as expected, shows that abscisic acid is accumulated under drought and that mutants affected in aspartate oxidase have an increased content in salicylate.
FP16314Endosperm-specific OsPYL8 and OsPYL9 act as positive regulators of the ABA signaling pathway in rice seed germination
Pyrabactin resistance-like (PYL) proteins were identified as receptors of the plant hormone ABA, but the functions of many PYLs remains to be elucidated. In this work, we determined that rice OsPYL8 and OsPYL9 are specifically expressed in the endosperms. Analyses of transgenic rice plants overexpressing OsPYL8 or OsPYL9 further showed that the two OsPYLs function as positive regulators of ABA signaling pathway in rice seed germination.
Although Crassulacean acid metabolism (CAM) has been well studied in the family Bromeliaceae, the relationship between CAM activity and vacuolar organic-acid transport at night has not. We investigated ATP- and PPi-dependent proton transport in the tonoplast membrane vesicles of seven bromeliad species. We found that tonoplast ATP-driven H+ activity is greater than tonoplast PPi. Our results demonstrate a close correlation between CAM rhythm and the intrinsic properties of vacuolar membranes.
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FP16437Molecular characterisation and expression profiling of calcineurin B-like (CBL) genes in Chinese cabbage under abiotic stresses
The CBL genes act as calcium sensors present in plants, therefore, identification of candidate CBL gene (s) confer the responses against abiotic stresses. We have identified BrCBL1-1, BrCBL9-1, and BrCBL4-2 as candidate genes for cold, salt, and drought stresses, respectively. Our results will help the molecular breeders for developing abiotic stress tolerance Chinese cabbage cultivars through conventional or gene engineering.
FP17051Performance of Arabidopsis thaliana under different light qualities: comparison of light-emitting diodes to fluorescent lamp
The change to LED systems for growing Arabidopsis thaliana raises questions concerning the reproducibility of results obtained under fluorescent light. We compared growth, photosynthesis and metabolite contents in Arabidopsis genotypes grown under two LED systems or fluorescent lamps. In conclusion, the effect caused by the change to LED was small compared to those of plant age and diurnal rhythm.
A new complementary flowering model of sugar beet was proposed. Investigations confirmed that AGLX2 was the first candidate lncRNA gene in Beta vulgaris and that the BvRAV1-like gene was expressed in response to vernalisation. Our findings opened up new possibility for future studies and further illuminated the molecular mechanism of vernalisation in sugar beet.
FP16418The levels of peroxisomal catalase protein and activity modulate the onset of cell death in tobacco BY-2 cells via reactive oxygen species levels and autophagy
Peroxisomes balance the cellular levels of reactive oxygen species (ROS) and therefore should modulate ROS-regulated programs like autophagy or cell death. We used tobacco suspension cultures to show that degradation of peroxisomes via autophagy was a prerequisite for cell death and depended on the levels of the major peroxisomal protein, catalase. This suggests a role of plant catalase in the regulation of peroxisome turnover and autophagic cell death.
FP16424Overexpression of GSK3-like Kinase 5 (OsGSK5) in rice (Oryza sativa) enhances salinity tolerance in part via preferential carbon allocation to root starch
The development of salt-tolerant rice cultivars is critical for its production in areas of high soil salinity. This study examined salt-adaptive mechanisms conferred by elevated levels of a regulatory protein OsGSK5, which enhanced rice salinity tolerance through the reallocation of carbon to root. This novel mechanism provides a target for rice breeders to develop cultivars that can withstand episodes of salinity.
FP16370Combined effects of soil salinity and high temperature on photosynthesis and growth of quinoa plants (Chenopodium quinoa)
The Andean crop species Chenopodium quinoa (Willd.) can cope with combined salinity and high temperature stress, albeit these factors have partially contradictory implications on photosynthesis. Morphological and physiological analysis revealed high phenotypic plasticity of the canopy, resulting in improved leaf gas exchange and maintenance of photosynthesis. Quinoa is thereby a suitable candidate for agriculture in regions affected by salinity and high temperature.
FP16041Changes in leaf stomatal conductance, petiole hydraulics and vessel morphology in grapevine (Vitis vinifera cv. Chasselas) under different light and irrigation regimes
Hydraulic conductance in plants may be affected by environmental factors, which in turn, regulate gas exchanges and yield. Leaf stomatal conductance and specific hydraulic conductivity in petioles (Kpetiole) were evaluated in grapevines under different radiation and water regimes. Results indicate that variations in Kpetiole were modulated in the short-term by the expression of aquaporins and in a longer-term, by modifications in the anatomy of xylem vessels.
FP16397Overexpressing OsMAPK12-1 inhibits plant growth and enhances resistance to bacterial disease in rice
Mitogen-activated protein kinases (MAPKs) play crucial roles in plant development and response to environmental stress. In our study, OsMAPK12-1 – an alternatively spliced form of BWMK1 – was found to respond to defence-related elicitors and positively modulates rice resistance against bacterial blight and streak disease whereas negatively regulates plant growth. Therefore, OsMAPK12-1 showed a balance between rice pathogen resistance and vegetative growth which provided a basis for rice molecular breeding.
Climate changes result in more floods also in regions where the mean precipitation is predicted to decline. Dryland crops such as wheat and barley are particularly vulnerable to submergence stress. Here, we show that superhydrophobic leaf surfaces enhance survival of completely submerged wheat by formation of thin leaf gas films that helps wheat to ‘breathe’ under water.
FP16434Histone modifications at the grapevine VvOMT3 locus, which encodes an enzyme responsible for methoxypyrazine production in the berry
Methoxypyrazines are responsible for some herbaceous characters in wines of some grape varieties characters and this is determined by the differential expression of a methyltransferase gene known as VvOMT3. In order to investigate the chromatin arrangement of the VvOMT3 gene, histone modifications were studied in the locus and these differed spatially between the skin and flesh tissues, temporally during fruit development and also amongst different VvOMT3 alleles. This study provides evidence of histone tail modification of the VvOMT3 locus in grapevine, which may play a role in the varietal, spatial and developmental regulation of the expression of this gene.
FP16339Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds
The electrophysiological response pattern of Nitellopsis obtusa cell can be assessed to evaluate the effect of many biologically active compounds. We illustrate a variety of electrophysiological approaches for the investigations of electrical signaling after chemical treatment in vivo. The insights about the Characean model system are likely to hold for plants in general and even deepen the understanding of the plant evolution.
FP16324Molecular insights into the functional role of nitric oxide (NO) as a signal for plant responses in chickpea
Although many studies established nitric oxide (NO) as a signaling molecule in plants, the identification of target molecules of NO has remained elusive due to the lack of in depth molecular studies. Our quantitative proteome analysis suggests the differential regulation of 248 proteins and dynamic regulation of metabolic pathways by exogenous NO donor in chickpea. This is the first report in legumes pointing at the potential candidates that attribute the reported functions of NO in plants.
FP16327Anatomical and biochemical characterisation of a barrier to radial O2 loss in adventitious roots of two contrasting Hordeum marinum accessions
A barrier to radial oxygen loss (ROL) in roots is an important adaptation of many wetland plants growing in waterlogged, anoxic soils; however, knowledge of the nature of the barrier is sparse. The ROL barrier enhances longitudinal oxygen diffusion through aerenchyma to the root tip. Our comparison of two Hordeum marinum accessions differing in ROL barrier strength showed that the deposition of suberin into walls of the root exodermis is associated with reduction in loss of oxygen from basal root zones to the external medium.
FP16379Photochemical activity changes accompanying the embryogenesis of pea (Pisum sativum) with yellow and green cotyledons
We studied the dynamics of photochemical activity in seed coats and cotyledons during development of yellow- and green-seeded pea cultivars by using the pulse amplitude modulation fluorometric analysis. The fast transients of the chlorophyll a fluorescence revealed higher photochemical activity in the coats of yellow-seeded cultivar at the early- and middle cotyledon stages of seed development in comparison to those observed in the green-seeded ones. Photochemical activity in the cotyledons of both cultivars could not be any more detected at the late cotyledon stage. This process was triggered by dehydration of seed tissues.
FP16357Environmental factors constraining adventitious root formation during flooding of Solanum dulcamara
Flooding generally poses a threat to terrestrial plants, but wetland species display adaptations that prevent damage by the adverse conditions imposed by floods. One of these adaptations – formation of adventitious roots that replace the original non-adapted root system – may be severely constrained if insufficient light or contact of leaves with the atmosphere is present during the flooding event. This results in significantly fewer adventitious roots growing out of the stem, and thus poor performance in these stressful conditions.
FP16340Cytokinins regulate root growth through its action on meristematic cell proliferation but not on the transition to differentiation
Root growth is controlled by phytohormones, but what cellular processes are regulated and how it occurs is still an open question. Here it is shown that cytokinin affects root growth mainly through its effect on cell proliferation, and does not initiate the transition of cells to differentiation as previously thought. Cellular analysis performed could be applied for the analysis of how any plant hormone influences developmental processes in plant roots.
Intracellular communications in plant cells of large dimensions rely primarily on cytoplasmic streaming, because diffusion is too slow for the transport on mm-scale distances. Illumination of a small cell spot at a various distances from the point of chlorophyll fluorescence measurements revealed the wave-like propagation of the fluorescence response along the cell length. The results show that the photosynthetic function of immobile chloroplasts under constant light can be affected by long-distance transmission of a photosynthetically active metabolite from the remote cell parts.
Some terrestrial plants, including wheat (Triticum aestivum), possess superhydrophobic leaf surfaces that retain a thin gas film when submerged. We tested gas film retention time of 14 different wheat cultivars and found that wheat could retain the gas films for a minimum of 2 days. We suggest that leaf gas film is a relevant trait to use as a selection criterion to improve the flood tolerance of crops that become temporarily submerged.
FP16279Molecular mechanisms accompanying nitric oxide signalling through tyrosine nitration and S-nitrosylation of proteins in plants
Understanding the molecular mechanisms of plant development constitutes an important field of investigations in the current era of plant biology research. Nitric oxide signalling regulates a variety of biochemical processes in plants. Present review provides an in-depth analysis of our current understanding on the subject, particularly with reference to plant growth under stress conditions.
Melatonin is an important hormone and signalling molecule in all forms of life including humans, plants and bacteria. Recent plant physiology and genomic experiments have described the redirection of plant growth and metabolism, and demonstrated a diversity of genes involved in response to melatonin, however, the exact metabolic cascades that translate melatonin signals into physiological responses is not fully understood. This review provides an overview of melatonin mediated signalling manifested as behaviours and its roles in basic and industrial research.
Memristors, or resistors with memory, exist in vivo as components of plasma membranes in plants, fruits, roots and seeds. Authors found memristors in an androecium, spur, petals and pedicel in Sunpatiens flowers. The discovery of memristors in Sunpatiens (Impatiens spp.) creates a new direction in the modelling and understanding of electrophysiological phenomena and memory elements in flowers.
Plant fibres with a tertiary cell wall (G-layer) may function as plant ‘muscles’. Large-scale transcriptome profiling of isolated flax phloem fibres permitted to identify the major players and regulatory elements that operate during graviresponce specifically in the fibres of the pulling stem side. The suggested mechanisms of phloem fibre involvement in tropisms may considerably renew the concept of herbaceous plant behaviour upon gravistimulation.
Clarifying herbivory-induced plant cellular signalling is a critical step to push the research of plant-herbivore interaction forward. We review the role of ion channels/transporters in modulating herbivory-induced early signalling events and rapid systemic signal transmission in plants. This work provides a comprehensive source of information about plant defensive strategies upon attack.
FP16292Studies of cytokinin receptor–phosphotransmitter interaction provide evidences for the initiation of cytokinin signalling in the endoplasmic reticulum
Cytokinin is an important plant hormone and its mode of action has been extensively studied; however, to date, the subcellular localisation of cytokinin perception and signal transduction remains a matter of debate. This study describes cytokinin receptor–phosphotransmitter interaction and its subcellular localisation in living plant cells and it provides several experimental evidences for receptor activity at the endoplasmic reticulum (ER) membrane. It is concluded that intracellular cytokinins within the ER lumen may play an important role in cytokinin signalling, at least in some cell types.
Rhizobium bacteria, which live within the root nodules of legumes, allow plants to capture nitrogen gas from the atmosphere and use it for their own growth. Central to this symbiosis is an intracellular structure, called the symbiosome, in which nitrogen-fixing bacterial cells exchange components with the host cells that harbor them. Recent research on the differentiation of symbiosomes and of the infected cells that accommodate them has helped to decipher some general molecular mechanisms of cell differentiation.
FP16342Parameters of electrical signals and photosynthetic responses induced by them in pea seedlings depend on the nature of stimulus
Plants, like animals, produce electrical signals in response to various external influences. In this study we raised a question whether the electrical signals transmit information about the nature of the stimulus, and found out that different stimuli induce signals of varied parameters. The obtained results explain how plants adapt to changing environment.
FP16380The role of ion disequilibrium in induction of root cell death and autophagy by environmental stresses
Environmental stresses are main causes for low agricultural productivity. At the cellular level, stresses induce generation of reactive oxygen species (ROS), ion disequilibrium, autophagy and programmed cell death (PCD). Here we propose that these processes interact and that ROS and ion disequilibrium are triggers of autophagy and PCD. Overall, presented data contribute to understanding plant stress physiology.
FP16420Mechanisms of cytosolic calcium elevation in plants: the role of ion channels, calcium extrusion systems and NADPH oxidase-mediated ‘ROS-Ca2+ Hub
Transient elevation of cytosolic Ca2+, also referred as a Ca2+signal, is as central phenomenon of plant signalling. Plants evolved sophisticated systems to initiate, amplify and terminate Ca2+ signals. Structure and properties of these systems, including Ca2+-permeable ion channels, Ca2+-ATPases, Ca2+/H+ exchangers and ‘ROS-Ca2+ hub’ are discussed here. They provide a fine-tuned mechanism for encoding diverse external and internal stimuli.
FP16347Spatial distribution of organelles in leaf cells and soybean root nodules revealed by focused ion beam-scanning electron microscopy
Focussed ion bean scanning electron microscopy (FIB-SEM) is a technique that can be used to generate 3D renderings of cells and their contents. Although FIB-SEM has been regularly used to investigate animal cells and tissues, it has rarely been deployed to study plant structures. Here we demonstrate that FIB-SEM can easily be used to study plant samples and have discovered previously unknown arrangements of organelles and membranes in those samples.
FP16322Formation mechanisms of superoxide radical and hydrogen peroxide in chloroplasts, and factors determining the signalling by hydrogen peroxide
Photosynthetic electron transport chain is not only source of ATP and NADPH for photosynthesis; it is a sensor, informing adaptation systems of plant about environmental changes. An important transmitter of this information is hydrogen peroxide whose mechanisms of formation are presented, laying special emphasis on the formation outside and within thylakoid membrane. It is discussed, that the formation place can ensure definite signal about the specific environmental change.
Although plant proteases of the phytaspase family are important contributors to stress-induced plant cell death, phytaspase of a classical model plant Arabidopsis thaliana has escaped identification thus far. We identified the Arabidopsis phytaspase-encoding gene and characterised the recombinant enzyme. Substrate specificity and properties of the Arabidopsis phytaspase display both important similarities with and distinctions from the already characterised phytaspases.
Cyclic GMP (cGMP) signalling in plants is crucial for many physiological processes. Recent analytical and genomic developments now allow detailed studies into the biochemistry and physiological role of cGMP in plants, and the latest findings are reviewed in this article.
Large conductance SV/TPC1 channels are ubiquitously and abundantly expressed in the vacuolar membranes of higher plants. They are unique established Ca2+-permeable channels in vacuoles, but their activity is strongly negatively controlled, so that they were believed to be inactive or to act only locally. Recent evidence suggests the key role of SV/TPC1 channels in the long-distance Ca2+ signalling.
FP16261Two native types of phytochrome A, phyAʹ and phyAʺ, differ by the state of phosphorylation at the N-terminus as revealed by fluorescence investigations of the Ser/Ala mutant of rice phyA expressed in transgenic Arabidopsis
Plants adapt to environmental light conditions with the use of the sophisticated phytochrome system. In this work, polymorphism of its major component – phytochrome A– was investigated. With the use of transgenic Arabidopsis and fluorescence technique, it was shown that two molecular types of the photoreceptor differ by the state of phosphorylation and their existence accounts for its complex functions.
FP16242Rapid changes in root HvPIP2;2 aquaporins abundance and ABA concentration are required to enhance root hydraulic conductivity and maintain leaf water potential in response to increased evaporative demand
The ABA-deficient barley mutant Az34 and wild type (WT) were exposed to air warming. Although transpiration rate of both genotypes increased, leaf water potential decreased in the mutant but was maintained in WT plants. Only WT plants showed increased root ABA accumulation, which increased root hydraulic conductivity and aquaporin abundance, which seems important in maintaining leaf hydration.
These articles have been peer reviewed and accepted for publication. They are still in production and have not been edited, so may differ from the final published form.
Unraveling the plant signalling machinery: an update on the cellular and genetic basis of plant signal transduction
Insights into the functional relationship between cytokinin-induced root system phenotypes and nitrate uptake in Brassica napus L.
Melatonin ameliorates aluminium toxicity through modulating antioxidative enzymes and enhancing organic acid anion exudation in soybean
Cellulosic fibers of flax recruit both primary and secondary cell wall CESAs during deposition of thick tertiary cell wall and in the course of graviresponse
Myotubularins, PtdIns5P, and ROS in ABA-Mediated Stomatal Movements in Dehydrated Arabidopsis Seedlings
Improvement of submergence tolerance in rice through efficient application of potassium under submergence-prone rainfed ecology of Indo-Gangetic Plains
Genotypic variation in carbon fixation, 13C fractionation and grain yield in seven wheat cultivars grown under well watered condition
Maximized photosynthetic capacity and decreased hydraulic failure risk during aging in the clump bamboo, Bambusa chungii
Plant ionic relation and whole-plant physiological responses to waterlogging, salinity and their combination in barley
Acclimation to light and avoidance of photoinhibition in Typha latifolia is associated with high photosynthetic capacity and xanthophyll pigment content
Multiple mechanisms mediate growth and survival in young seedlings of two populations of the halophyte Atriplex halimus (L.) subjected to long single step salinity treatments.
Contrasting oxygen dynamics in Limonium narbonense and Sarcocornia fruticosa during partial and complete submergence
Metabolomics analysis of post-photosynthetic effects of gaseous O2 on primary metabolism in illuminated leaves
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Functional Plant Biology 44 (1)Nicolas Virlet, Kasra Sabermanesh, Pouria Sadeghi-Tehran, Malcolm J. Hawkesford
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Consequences of phloem pathway unloading/reloading on equilibrium flows between source and sink: a modelling approachFunctional Plant Biology 44 (5)Peter E. H. Minchin, André Lacointe
Use of multicolour fluorescence imaging for diagnosis of bacterial and fungal infection on zucchini by implementing machine learningFunctional Plant Biology 44 (6)Mónica Pineda, María Luisa Pérez-Bueno, Vanessa Paredes, Matilde Barón
Genotypic variation in soil water use and root distribution and their implications for drought tolerance in chickpeaFunctional Plant Biology 44 (2)Ramamoorthy Purushothaman, Lakshmanan Krishnamurthy, Hari D. Upadhyaya, Vincent Vadez, Rajeev K. Varshney
Photosynthetic acclimation to elevated CO2 concentration in a sweet pepper (Capsicum annuum) crop under Mediterranean greenhouse conditions: influence of the nitrogen source and salinityFunctional Plant Biology 44 (6)Manuel E. Porras, Pilar Lorenzo, Evangelina Medrano, María J. Sánchez-González, Ginés Otálora-Alcón, María C. Piñero, Francisco M. del Amor, M. Cruz Sánchez-Guerrero
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Genotypic water-deficit stress responses in durum wheat: association between physiological traits, microRNA regulatory modules and yield componentsFunctional Plant Biology 44 (5)Haipei Liu, Amanda J. Able, Jason A. Able
Truncation of grain filling in wheat (Triticum aestivum) triggered by brief heat stress during early grain filling: association with senescence responses and reductions in stem reservesFunctional Plant Biology 43 (10)Hamid Shirdelmoghanloo, Daniel Cozzolino, Iman Lohraseb, Nicholas C. Collins
Active defence by an Australian native host, Lomandra longifolia, provides resistance against Phytophthora cinnamomiFunctional Plant Biology 44 (4)Md Tohidul Islam, James E. Rookes, David M. Cahill
Endosperm-specific OsPYL8 and OsPYL9 act as positive regulators of the ABA signaling pathway in rice seed germinationFunctional Plant Biology 44 (6)Ziqiang Chen, Lan Kong, Yun Zhou, Zaijie Chen, Dagang Tian, Yan Lin, Feng Wang, Songbiao Chen
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Plants in constrained canopy micro-swards compensate for decreased root biomass and soil exploration with increased amounts of rhizosphere carboxylatesFunctional Plant Biology 44 (5)Robert P. Jeffery, Richard J. Simpson, Hans Lambers, Daniel R. Kidd, Megan H. Ryan
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