Dual energy X-ray absorptiometry predicts the effects of dietary protein on body composition of pigs
D. Suster A B , B. J. Leury B , D. J. Kerton A and F. R. Dunshea A B CA Department of Primary Industries, 600 Sneydes Road, Werribee, Vic. 3030, Australia.
B Institute of Land and Food Resources, The University of Melbourne, Parkville, Vic. 3010, Australia.
C Corresponding author. Email: frank.dunshea@dpi.vic.gov.au
Australian Journal of Experimental Agriculture 46(11) 1439-1445 https://doi.org/10.1071/EA04266
Submitted: 8 December 2004 Accepted: 15 May 2004 Published: 9 October 2006
Abstract
The present study investigated the potential of dual energy X-ray absorptiometry to determine the effect of dietary protein on live pig body composition compared with the standard methods. The experiment utilised 48 Large White × Landrace gilts, stratified on liveweight at 17 weeks of age (initial liveweight about 60 kg), and allocated within strata to diets containing either 85 or 100% of dietary protein requirements. The diets were fed ad libitum and contained 14.6 or 16.7% protein, respectively. Pigs were housed individually to allow weekly measurement of feed intake and liveweight. A Hologic QDR4500A dual energy X-ray absorptiometer was used to determine lean, fat and ash composition of pigs initially and 4 weeks later at the end of the experiment. Daily gain was increased by about 200 g in pigs fed the protein-adequate diet, but feed intake was not affected. Therefore, pigs fed adequate protein had a lower feed conversion ratio (2.92 v. 3.52 g/g, P<0.001). Feeding the protein-adequate diet increased lean deposition by about 150 g/day (577 v. 429 g/day, P<0.001) and ash deposition by about 4 g/day (28.7 v. 25.0 g/day, P<0.001), but did not affect fat accretion. This resulted in carcasses with a higher lean content (54.0 v. 50.7 kg, P<0.001) and ash content (2.35 v. 2.28 kg, P<0.05) but unaltered fat content. Therefore, an inadequate level of dietary protein leads to suboptimal growth in lean tissue and bone mineral, with no pronounced effect on fat. These observations were substantiated by chemical analysis and available corrective equations were useful in correcting differences between dual energy X-ray absorptiometry outputs and chemical values. After correction, dual energy X-ray absorptiometry estimates differed less than 5% from the chemical values for lean, protein, water and ash and 10% for lipid. In addition, reduced standard error of the differences around most dual energy X-ray absorptiometry measurements, relative to chemical analysis, allowed small changes in body composition to be detected with increased confidence. These data support the efficacy of using dual energy X-ray absorptiometry in nutritional studies of pigs.
Acknowledgments
The first author would like to thank Australian Pork Limited (APL) for the provision of a post-graduate student stipend.
Atkinson T,
Fowler VR,
Garton GA, Lough AKA
(1972) Rapid method for the accurate determination of lipid in animal tissues. The Analyst 97, 562–568.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Campbell RG,
Taverner MR, Curic DM
(1984) Effect of feeding level and dietary protein content on the growth, body composition and rate of protein deposition in pigs growing from 45 to 90 kg. Animal Production 38, 233–240.
Campbell RG,
Johnson RJ,
King RH,
Taverner MR, Meisinger DJ
(1990) Interaction of dietary protein content and exogenous porcine growth hormone administration on protein and lipid accretion rates in growing pigs. Journal of Animal Science 68, 3217–3225.
| PubMed |
Chen HY,
Lewis AJ,
Miller PS, Yen JT
(1999) The effect of excess protein on growth performance and protein metabolism of finishing barrows and gilts. Journal of Animal Science 77, 3238–3247.
| PubMed |
Critser DJ,
Miller PS, Lewis AJ
(1995) The effects of dietary protein concentration on compensatory growth in barrows and gilts. Journal of Animal Science 73, 3376–3383.
| PubMed |
Dunshea FR,
King RH, Campbell RG
(1993) Interrelationships between dietary protein and ractopamine on protein and lipid deposition in finishing gilts. Journal of Animal Science 71, 2931–2941.
| PubMed |
Edmonds MS,
Arentson BE, Mente GA
(1998) Effect of protein levels and space allocations on performance of growing-finishing pigs. Journal of Animal Science 76, 814–821.
| PubMed |
Friesen KG,
Nelssen JL,
Goodband RD,
Tokach MD,
Unruh JA,
Kropf DH, Kerr BJ
(1995) The effect of dietary lysine on growth, carcass composition, and lipid metabolism in high-lean growth gilts fed from 72 to 136 kilograms. Journal of Animal Science 73, 3392–3401.
| PubMed |
de Greef KH,
Kemp B, Verstegen MWA
(1992) Performance and body composition of fattening pigs of two strains during protein deficiency and subsequent realimentation. Livestock Production Science 30, 141–153.
| Crossref | GoogleScholarGoogle Scholar |
Kelly TL,
Berger N, Richardson TL
(1998) DXA body composition: theory and practice. Applied Radiation and Isotopes 49, 511–513.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Kempster AJ, Evans DG
(1979) A comparison of different predictors of the lean content of pig carcasses. 1. Predictors for use in commercial classification and grading. Animal Production 28, 87–96.
King RH,
Campbell RG,
Smits RJ,
Morley WC,
Ronnfeldt K,
Butler K, Dunshea FR
(2000) Interrelationships between dietary lysine, sex, and porcine somatotropin administration on growth performance and protein deposition in pigs between 80 and 120 kg live weight. Journal of Animal Science 78, 2639–2651.
| PubMed |
Mitchell AD,
Scholz AM,
Pursel VG, Evock-Clover CM
(1998a) Composition analysis of pork carcasses by dual-energy x-ray absorptiometry. Journal of Animal Science 76, 2104–2114.
| PubMed |
Mitchell AD,
Scholz AM,
Pursel VG, Evock-Clover CM
(1998b) Incremental changes in total and regional body composition of growing pigs measured by Dual-energy X-ray absorptiometry. Growth, Development, and Aging 60, 95–105.
Suster D,
Leury BJ,
Ostrowska E,
Butler KL,
Kerton DJ,
Wark JD, Dunshea FR
(2003) Accuracy of dual energy X-ray absorptiometry (DXA), weight and P2 back fat to predict whole body and carcass composition in pigs within and across experiments. Livestock Production Science 84, 231–242.
| Crossref | GoogleScholarGoogle Scholar |
Suster D,
Leury BJ,
Hofmeyr CD, Dunshea FR
(2004a) The accuracy of dual energy X-ray absorptiometry (DXA), weight and P2 back fat to predict half-carcass and primal-cut composition in pigs within and across research experiments. Australian Journal of Agricultural Research 55, 973–982.
| Crossref | GoogleScholarGoogle Scholar |
Suster D,
Leury BJ,
King RH,
Mottram M, Dunshea FR
(2004b) Interrelationships between porcine somatotropin (pST), betaine and energy level on body composition and tissue distribution of finisher boars. Australian Journal of Agricultural Research 55, 983–990.
| Crossref | GoogleScholarGoogle Scholar |
Szabo C,
Jansman AJ,
Babinszky L,
Kanis E, Verstegen MW
(2001) Effect of dietary protein source and lysine:DE ratio on growth performance, meat quality, and body composition of growing-finishing pigs. Journal of Animal Science 79, 2857–2865.
| PubMed |
Tuitoek JK,
Young LG,
de Lange CF, Kerr BJ
(1997) Body composition and protein and fat accretion in various body components in growing gilts fed diets with different protein levels but estimated to contain similar levels of ideal protein. Journal of Animal Science 75, 1584–1590.
| PubMed |
Tyler RW,
Luce WG,
Johnson RK,
Maxwell CV,
Hintz RL, Walters LE
(1983) The effects of level of crude protein on performance of growing boars Journal of Animal Science 57, 364–372.
| PubMed |
Zervas S, Zijlstra RT
(2002a) Effects of dietary protein and fermentable fiber on nitrogen excretion patterns and plasma urea in grower pigs. Journal of Animal Science 80, 3247–3256.
| PubMed |
Zervas S, Zijlstra RT
(2002b) Effects of dietary protein and oathull fiber on nitrogen excretion patterns and postprandial plasma urea profiles in grower pigs. Journal of Animal Science 80, 3238–3246.
| PubMed |
