Successful stem cutting propagation of chickpea, its wild relatives and their interspecific hybrids
N. Danehloueipour A B C , G. Yan A B , H. J. Clarke B and K. H. M. Siddique BA School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
B Centre for Legumes in Mediterranean Agriculture (CLIMA), Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
C Corresponding author. Email: ndaneh@plants.uwa.edu.au
Australian Journal of Experimental Agriculture 46(10) 1349-1354 https://doi.org/10.1071/EA05207
Submitted: 4 August 2004 Accepted: 17 March 2005 Published: 13 September 2006
Abstract
A successful stem cutting method was developed to propagate chickpea (Cicer arietinum L.), its crossable wild annual relatives (C. reticulatum Ladiz. and C. echinospermum P.H. Davis) and their interspecific hybrids. The effect of plant growth regulator powder (0.5 mg/g indole butyric acid and 0.5 mg/g naphthalene acetic acid), honey, combined honey + plant growth regulator powder, different growth stages of the donor plant, and rooting substrates on rooting rate, root number, root length, and survival rate were investigated. The highest propagation success rate was achieved when cuttings were taken at the pre-flowering stage, treated with plant growth regulator powder and grown in a sand + potting mix substrate. The rooting rate ranged from 87.5 to 100% for chickpea, C. reticulatum and C. echinospermum, and interspecific hybrids. All of the accessions examined in the study were successfully propagated with the new method. This study provides a simple and efficient technique for vegetative propagation of Cicer species which will be useful for the multiplication of seed, production of clones for disease screening or for the development of mapping populations.
Additional keyword: plant growing stage.
Acknowledgments
We thank Ms Leila Eshraghi for her technical assistance. We also thank Dr Fucheng Shan for providing wild annual Cicer accessions. This research was partially funded by the Ministry of Science, Research, and Technology of Iran.
Balabushka VK
(1984) Application of growth regulators in the vegetative propagation of Buxus sempervirens. Introduktsiya i Akklimatizatsiya Rastenii. Kiev, Ukrainian SSR 2, 26–27.
(verified 12 June 2006)
Islam R, Riazuddin S
(1993) In vitro rooting on micropropagated shoots of chickpea. International Chickpea Newsletter 29, 21–23.
van der Maesen LJG
(1980) Growing wild chickpeas. International Chickpea Newsletter 2, 3–4.
Muehlbauer FJ,
Kaiser WJ, Simon CJ
(1994) Potential for wild species in cool season food legume breeding. Euphytica 73, 109–114.
| Crossref | GoogleScholarGoogle Scholar |
Rupela OP
(1982) Rooting chickpea cuttings from field-grown plants. International Chickpea Newsletter 7, 9–10.
Rupela OP, Dart PJ
(1981) Vegetative propagation of chickpea. International Chickpea Newsletter 4, 12–13.
Sheila VK,
Moss JP,
Gowda CLL, van Rheenen HA
(1991) A protocol for micropropagation of Cicer spp. International Chickpea Newsletter 24, 28–30.
Siddique KHM, Sykes J
(1997) Pulse production in Australia past, present and future. Australian Journal of Experimental Agriculture 37, 103–111.
| Crossref | GoogleScholarGoogle Scholar |
Singh KB,
Ocampo B, Robertson LD
(1998) Diversity for abiotic and biotic stress resistance in the wild annual Cicer species. Genetic Resources and Crop Evolution 45, 9–17.
| Crossref | GoogleScholarGoogle Scholar |
Syed H,
Ahsan-ul-Haq M, Shah TM
(2002) Vegetative propagation of chickpea (Cicer arietinum L.) through stem cuttings. Asian Journal of Plant Sciences 1, 218–219.
Wamatu JN, King'oro MW
(1992) Vegetative propagation of cultivar Ruiru 11 by rooting softwood stem cuttings. Kenya Coffee 57, 1399–1404.