The Arabidopsis selenium-binding protein confers tolerance to toxic levels of selenium
Adamantia Agalou A , Andreas Roussis A B and Herman P. Spaink A CA Institute of Biology, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands.
B Current address: Center for Human and Clinical Genetics, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands.
C Corresponding author. Email: spaink@rulbim.leidenuniv.nl
Functional Plant Biology 32(10) 881-890 https://doi.org/10.1071/FP05090
Submitted: 18 April 2005 Accepted: 2 June 2005 Published: 5 October 2005
Abstract
In the Arabidopsis genome there are three highly conserved homologues of the mammalian 56-kD selenium-binding protein (SBP). To study the function of SBP in this model plant, we used a transgenic approach by constitutively overexpressing and down-regulating the endogenous Atsbp1 gene. In the latter case, we employed both a conventional antisense method and gene silencing by intron-containing hairpin RNAs. Atsbp1-overexpressing and silenced plants were phenotypically normal, under standard growth conditions, when compared with wild type plants. Transgenic plants exhibited different growth responses to exogenously supplied selenite, which correlated with the expression levels of Atsbp1. Plants with increased Atsbp1 transcript levels showed enhanced tolerance to selenite, while plants with reduced levels were more sensitive. Our results indicate that, although Atsbp1 does not play a detectable role in the regulation of developmental processes under normal growth conditions, it appears to be involved in processes controlling tolerance of Arabidopsis to selenium toxicity.
Keywords: Arabidopsis, selenium-binding protein, selenium toxicity.
Acknowledgments
We thank Dr Renze Heidstra (Utrecht University) and Dr Adam Vivian-Smith (Leiden University) for their help in phenotypic characterisation of transgenic plants. Adamantia Agalou was supported from the State Scholarships Foundation of Greece (IKY).
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