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Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Simultaneous recording of diurnal changes in leaf turgor pressure and stem water status of bread wheat reveal variation in hydraulic mechanisms in response to drought

Helen Bramley A E , Rebecca Bitter B C D , Gertraud Zimmermann B C and Ulrich Zimmermann B C
+ Author Affiliations
- Author Affiliations

A Plant Breeding Institute, Faculty of Agriculture and Environment, The University of Sydney, 12 656 Newell Highway, Narrabri, NSW 2390, Australia.

B ZIM-Plant Technology GmbH, Neuendorfstr. 19, 16761 Hennigsdorf, Germany.

C Department of Biotechnology, University Würzburg, Biocenter, Am Hubland D-97 074 Würzburg, Germany.

D Present address: YARA ZIM Plant Technology GmbH, Neuendorfstr. 19, 16 761 Hennigsdorf, Germany.

E Corresponding author. Email: helen.bramley@sydney.edu.au

Functional Plant Biology 42(10) 1001-1009 https://doi.org/10.1071/FP15087
Submitted: 31 March 2015  Accepted: 6 July 2015   Published: 24 August 2015

Abstract

Information about water relations within crop canopies is needed to improve our understanding of canopy resource distribution and crop productivity. In this study, we examined the dehydration/rehydration kinetics of different organs of wheat plants using ZIM-probes that continuously monitor water status non-destructively. ZIM-probes were clamped to the flag leaf and penultimate leaf of the same stem to monitor changes in turgor pressure, and a novel stem probe was clamped to the peduncle (just below the spike of the same stem) to monitor changes in stem water status. All organs behaved similarly under well-watered conditions, dehydrating and recovering at the same times of day. When water was withheld, the behaviour diverged, with the leaves showing gradual dehydration and incomplete recovery in leaf turgor pressure during the night, but the stem was affected to a lesser extent. Penultimate leaves were the most severely affected, reaching turgor loss point before the flag leaf. Upon rewatering, turgor pressure recovered but the output patch-pressure of the probes (Pp) oscillated at ~30 min periods in all organs of most plants (n = 4). Oscillations in Pp were attributed to oscillations in stomatal opening and appear to only occur above a threshold light intensity. The mechanisms identified in this study will be beneficial for crop productivity because the flag leaf is the source of most photoassimilates in developing grains, so the plant’s ability to maintain flag leaf hydration at the expense of older leaves should moderate the impact of drought on yield. Stomatal oscillations could increase water use efficiency as the plant attempts to rehydrate after drought.

Additional keywords: stem-probe, stomatal oscillations, water relations, zero turgor pressure, ZIM-probe.


References

Aggarwal PK, Sinha SK (1984) Differences in water relations and physiological characteristics in leaves of wheat associated with leaf position on the plant. Plant Physiology 74, 1041–1045.
Differences in water relations and physiological characteristics in leaves of wheat associated with leaf position on the plant.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXktVCjurc%3D&md5=04fe114fe5da6cedf040217412f66878CAS | 16663500PubMed |

Anten NPR, Schieving F, Werger MJA (1995) Patterns of light and nitrogen distribution in relation to whole-canopy carbon gain in C3 and C4 mono- and dicotyledonous species. Oecologia 101, 504–513.
Patterns of light and nitrogen distribution in relation to whole-canopy carbon gain in C3 and C4 mono- and dicotyledonous species.Crossref | GoogleScholarGoogle Scholar |

Bader MKF, Ehrenberger W, Bitter R, Stevens J, Miller BP, Chopard J, Rüger S, Hardy GESJ, Poot P, Dixon KW, Zimmermann U, Veneklaas EJ (2014) Spatio-temporal water dynamics in mature Banksia menziesii trees during drought. Physiologia Plantarum 152, 301–315.
Spatio-temporal water dynamics in mature Banksia menziesii trees during drought.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhsFCjtLzO&md5=071324761c1516d0279e665a3b257368CAS |

Ball RA, Oosterhuis DM (2005) Measurement of root and leaf osmotic potential using the vapor-pressure osmometer. Environmental and Experimental Botany 53, 77–84.
Measurement of root and leaf osmotic potential using the vapor-pressure osmometer.Crossref | GoogleScholarGoogle Scholar |

Barlow E, Lee J, Munns R, Smart M (1980) Water relations of the developing wheat grain. Australian Journal of Plant Physiology 7, 519–525.
Water relations of the developing wheat grain.Crossref | GoogleScholarGoogle Scholar |

Barrs HD (1971) Cyclic variations in stomatal aperture, transpiration, and leaf water potential under constant environmental conditions. Annual Review of Plant Physiology 22, 223–236.
Cyclic variations in stomatal aperture, transpiration, and leaf water potential under constant environmental conditions.Crossref | GoogleScholarGoogle Scholar |

Benkert R, Balling A, Zimmermann U (1991) Direct measurements of the pressure and flow in the xylem vessels of Nicotiana tabacum and their dependence on flow resistance and transpiration rate. Botanica Acta 104, 423–432.
Direct measurements of the pressure and flow in the xylem vessels of Nicotiana tabacum and their dependence on flow resistance and transpiration rate.Crossref | GoogleScholarGoogle Scholar |

Boyer JS (1995) Pressure probe. In ‘Measuring water status of plants and soils’. pp. 103–142. (Academic Press: San Diego, CA, USA)

Boyer JS, Knipling EB (1965) Isopiestic technique for measuring leaf water potentials with a thermocouple psychrometer. Proceedings of the National Academy of Sciences of the United States of America 54, 1044–1051.

Bramley H, Turner DW, Tyerman SD, Turner NC (2007a) Water flow in the roots of crop species: the influence of root structure, aquaporin activity, and waterlogging. Advances in Agronomy 96, 133–196.
Water flow in the roots of crop species: the influence of root structure, aquaporin activity, and waterlogging.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXktlyisL8%3D&md5=e9e6b347a7ffd208093962a6f5b966cdCAS |

Bramley H, Turner NC, Turner DW, Tyerman SD (2007b) Comparison between gradient-dependent hydraulic conductivities of roots using the root pressure probe: the role of pressure propagations and implications for the relative roles of parallel radial pathways. Plant, Cell & Environment 30, 861–874.
Comparison between gradient-dependent hydraulic conductivities of roots using the root pressure probe: the role of pressure propagations and implications for the relative roles of parallel radial pathways.Crossref | GoogleScholarGoogle Scholar |

Bramley H, Ehrenberger W, Zimmermann U, Palta JAP, Rüger S, Siddique KHM (2013) Non-invasive pressure probes magnetically clamped to leaves to monitor the water status of wheat. Plant and Soil 369, 257–268.
Non-invasive pressure probes magnetically clamped to leaves to monitor the water status of wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtVyqtL7F&md5=e499f30cf0db0f4fad80469160e86de9CAS |

Brodribb TJ, Holbrook NM (2005) Water stress deforms tracheids peripheral to the leaf vein of a tropical conifer. Plant Physiology 137, 1139–1146.
Water stress deforms tracheids peripheral to the leaf vein of a tropical conifer.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXislOqs7k%3D&md5=e9c58269446dee89dabf25bd18f2118fCAS | 15734905PubMed |

Caldeira CF, Jeanguenin L, Chaumont F, Tardieu F (2014) Circadian rhythms of hydraulic conductance and growth are enhanced by drought and improve plant performance. Nature Communications 5,
Circadian rhythms of hydraulic conductance and growth are enhanced by drought and improve plant performance.Crossref | GoogleScholarGoogle Scholar | 25370944PubMed |

Chen J-L, Reynolds J, Harley P, Tenhunen J (1993) Coordination theory of leaf nitrogen distribution in a canopy. Oecologia 93, 63–69.
Coordination theory of leaf nitrogen distribution in a canopy.Crossref | GoogleScholarGoogle Scholar |

Ehlert C, Maurel C, Tardieu F, Simonneau T (2009) Aquaporin-mediated reduction in maize root hydraulic conductivity impacts cell turgor and leaf elongation even without changing transpiration. Plant Physiology 150, 1093–1104.
Aquaporin-mediated reduction in maize root hydraulic conductivity impacts cell turgor and leaf elongation even without changing transpiration.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXnsleiu78%3D&md5=b860131533e343baddba438f195e4e7bCAS | 19369594PubMed |

Ehrenberger W, Rüger S, Rodríguez-Domínguez CM, Díaz-Espejo A, Fernández JE, Moreno J, Zimmermann D, Sukhorukov VL, Zimmermann U (2012) Leaf patch clamp pressure probe measurements on olive leaves in a nearly turgorless state. Plant Biology 14, 666–674.
Leaf patch clamp pressure probe measurements on olive leaves in a nearly turgorless state.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC38vhsFSnsA%3D%3D&md5=a7d0e3633037b16b6600a2d00ffe53d6CAS | 22288430PubMed |

Evans L, Rawson H (1970) Photosynthesis and respiration by the flag leaf and components of the ear during grain development in wheat. Australian Journal of Biological Sciences 23, 245–254.

Evans L, Wardlaw IF, Fischer R (1980) Wheat. In ‘Crop physiology: some case histories’. (Ed. L Evans) pp. 101–149. (Cambridge University Press: Cambridge, UK)

FAO (2015) ‘FAOSTAT.’ Available at http://faostat3.fao.org/browse/Q/QC/E [Verfied 27 July 15]

Farquhar GD, Cowan IR (1974) Oscillations in stomatal conductance: the influence of environmental gain. Plant Physiology 54, 769–772.
Oscillations in stomatal conductance: the influence of environmental gain.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cnhtFWmsw%3D%3D&md5=c67c86e17a0276c034bc8c42827d8e0fCAS | 16658969PubMed |

Farooq M, Hussain M, Siddique KHM (2014) Drought stress in wheat during flowering and grain-filling periods. Critical Reviews in Plant Sciences 33, 331–349.
Drought stress in wheat during flowering and grain-filling periods.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXoslOqtLc%3D&md5=ceeeaa20a54a750399ae43d5b0077096CAS |

Fernández JE, Rodriguez-Dominguez CM, Perez-Martin A, Zimmermann U, Rüger S, Martín-Palomo MJ, Torres-Ruiz JM, Cuevas MV, Sann C, Ehrenberger W, Diaz-Espejo A (2011) Online-monitoring of tree water stress in a hedgerow olive orchard using the leaf patch clamp pressure probe. Agricultural Water Management 100, 25–35.
Online-monitoring of tree water stress in a hedgerow olive orchard using the leaf patch clamp pressure probe.Crossref | GoogleScholarGoogle Scholar |

Frensch J, Hsiao TC (1993) Hydraulic propagation of pressure along immature and mature xylem vessels of roots of Zea mays measured by pressure-probe techniques. Planta 190, 263–270.
Hydraulic propagation of pressure along immature and mature xylem vessels of roots of Zea mays measured by pressure-probe techniques.Crossref | GoogleScholarGoogle Scholar |

Hellkvist J, Richards GP, Jarvis PG (1974) Vertical gradients of water potential and tissue water relations in Sitka spruce trees measured with the pressure chamber. Journal of Applied Ecology 11, 637–667.
Vertical gradients of water potential and tissue water relations in Sitka spruce trees measured with the pressure chamber.Crossref | GoogleScholarGoogle Scholar |

Huber B, Schmidt E (1936) Weitere thermo-elektrische Untersuchungen uber den Transpirationsstrom der Baume. Tharandt Forst Jahrbuch 87, 369–412.

Jordan WR, Brown KW, Thomas JC (1975) Leaf age as a determinant in stomatal control of water loss from cotton during water stress. Plant Physiology 56, 595–599.
Leaf age as a determinant in stomatal control of water loss from cotton during water stress.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cnhtFGgsQ%3D%3D&md5=61520e4dbda9246c11adb128fdaac2d5CAS | 16659351PubMed |

Kaiser H, Kappen L (2001) Stomatal oscillations at small apertures: indications for a fundamental insufficiency of stomatal feedback‐control inherent in the stomatal turgor mechanism. Journal of Experimental Botany 52, 1303–1313.
Stomatal oscillations at small apertures: indications for a fundamental insufficiency of stomatal feedback‐control inherent in the stomatal turgor mechanism.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXlsVCms7c%3D&md5=3bf537ad81e6b1576642ade9ea6cea9dCAS | 11432949PubMed |

Koide R, Robichaux R, Morse S, Smith C (1989) Plant water status, hydraulic resistance and capacitance. In ‘Plant physiological ecology’. (Eds R Pearcy, J Ehleringer, H Mooney, P Rundel) pp. 161–183. (Springer: Dordrecht, The Netherlands)

Kozlowski TT (1972) Shrinking and swelling of plant tissues. In ‘Plant responses and control of water balance. Vol. 3’. (Ed. TT Kozlowski.) pp. 1–64. (Academic Press: New York, NY, USA)

Kuchenbrod E, Landeck M, Thürmer F, Haase A, Zimmermann U (1996) Measurement of water flow in the xylem vessels of intact maize plants using flow-sensitive NMR imaging. Botanica Acta 109, 184–186.
Measurement of water flow in the xylem vessels of intact maize plants using flow-sensitive NMR imaging.Crossref | GoogleScholarGoogle Scholar |

Landsberg JJ, Sands P (2010) ‘Physiological ecology of forest production: principles, processes and models.’ (Elsevier Science and Technology: Saint Louis, MO, USA)

Marenco RA, Siebke K, Farquhar GD, Ball MC (2006) Hydraulically based stomatal oscillations and stomatal patchiness in Gossypium hirsutum. Functional Plant Biology 33, 1103–1113.
Hydraulically based stomatal oscillations and stomatal patchiness in Gossypium hirsutum.Crossref | GoogleScholarGoogle Scholar |

McAinsh MR, Webb A, Taylor JE, Hetherington AM (1995) Stimulus-induced oscillations in guard cell cytosolic free calcium. The Plant Cell 7, 1207–1219.
Stimulus-induced oscillations in guard cell cytosolic free calcium.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXnvVWnur0%3D&md5=b19ff5f741dbb94cc6734f2831ccb842CAS | 12242404PubMed |

Molz FJ, Klepper B (1973) On the mechanism of water-stress-induced stem deformation. Agronomy Journal 65, 304–306.
On the mechanism of water-stress-induced stem deformation.Crossref | GoogleScholarGoogle Scholar |

Morgan JA (1986) The effects of N nutrition on the water relations and gas exchange characteristics of wheat (Triticum aestivum L.). Plant Physiology 80, 52–58.
The effects of N nutrition on the water relations and gas exchange characteristics of wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL28XhtVymsrg%3D&md5=7404c05a8e756166cf22a1d44f2953b6CAS | 16664606PubMed |

Perämäki M, Vesala T, Nikinmaa E (2005) Modeling the dynamics of pressure propagation and diameter variation in tree sapwood. Tree Physiology 25, 1091–1099.
Modeling the dynamics of pressure propagation and diameter variation in tree sapwood.Crossref | GoogleScholarGoogle Scholar | 15996952PubMed |

Prytz G, Futsaether CM, Johnsson A (2003) Self-sustained oscillations in plant water regulation: induction of bifurcations and anomalous rhythmicity. New Phytologist 158, 259–267.
Self-sustained oscillations in plant water regulation: induction of bifurcations and anomalous rhythmicity.Crossref | GoogleScholarGoogle Scholar |

Raschke K (1979) Movements using turgor mechanisms: movements of stomata. In ‘Encyclopedia of plant physiology. Vol. 7’. (Eds W Haupt, ME Feinleib) pp. 383–441. (Springer: Berlin)

Rüger S, Netzer Y, Westhoff M, Zimmermann D, Reuss R, Ovadya S, Gessner P, Zimmermann G, Schwartz A, Zimmermann U (2010) Remote monitoring of leaf turgor pressure of grapevines subjected to different irrigation treatments using the leaf patch clamp pressure probe. Australian Journal of Grape and Wine Research 16, 405–412.
Remote monitoring of leaf turgor pressure of grapevines subjected to different irrigation treatments using the leaf patch clamp pressure probe.Crossref | GoogleScholarGoogle Scholar |

Savage MJ, Wiebe HH, Cass A (1983) In situ field measurement of leaf water potential using thermocouple psychrometers. Plant Physiology 73, 609–613.
In situ field measurement of leaf water potential using thermocouple psychrometers.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cnhsFCguw%3D%3D&md5=799f4012a4f5437c8368d3ae036005a6CAS | 16663267PubMed |

Scholander PF, Bradstreet ED, Hemmingsen EA, Hammel HT (1965) Sap pressure in vascular plants. Science 148, 339–346.
Sap pressure in vascular plants.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cvlsVKquw%3D%3D&md5=c6978401b8ccd3ce9d6dfc00f3adf560CAS | 17832103PubMed |

Scoffoni C, Vuong C, Diep S, Cochard H, Sack L (2014) Leaf shrinkage with dehydration: coordination with hydraulic vulnerability and drought tolerance. Plant Physiology 164, 1772–1788.
Leaf shrinkage with dehydration: coordination with hydraulic vulnerability and drought tolerance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXmsV2js70%3D&md5=7ba564dd3b715b473a68f48af27d0110CAS | 24306532PubMed |

Shackel KA (1984) Theoretical and experimental errors for in situ measurements of plant water potential. Plant Physiology 75, 766–772.
Theoretical and experimental errors for in situ measurements of plant water potential.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cnhsFyksw%3D%3D&md5=4dd28bf75d9b81fcb8f57a1fec48d6eaCAS | 16663701PubMed |

Sibounheuang V, Basnayake J, Fukai S (2006) Genotypic consistency in the expression of leaf water potential in rice (Oryza sativa L.). Field Crops Research 97, 142–154.
Genotypic consistency in the expression of leaf water potential in rice (Oryza sativa L.).Crossref | GoogleScholarGoogle Scholar |

Steppe K, Dzikiti S, Lemeur R, Milford JR (2006) Stomatal oscillations in orange trees under natural climatic conditions. Annals of Botany 97, 831–835.
Stomatal oscillations in orange trees under natural climatic conditions.Crossref | GoogleScholarGoogle Scholar | 16478765PubMed |

Steudle E (1993) Pressure probe techniques: basic principles and application to studies of water and solute relations at the cell, tissue and organ level. In ‘Water deficits. Plant responses from cell to community’. (Eds JAC Smith, H Griffiths) pp. 5–36. (Bios Science: Oxford)

Turner NC (1974) Stomatal behavior and water status of maize, sorghum, and tobacco under field conditions: II. At low soil water potential. Plant Physiology 53, 360–365.
Stomatal behavior and water status of maize, sorghum, and tobacco under field conditions: II. At low soil water potential.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cnhtVyrug%3D%3D&md5=3648cb51e4decd78608d3b544f1ba6d3CAS | 16658706PubMed |

Turner NC (1981) Techniques and experimental approaches for the measurement of plant water status. Plant and Soil 58, 339–366.
Techniques and experimental approaches for the measurement of plant water status.Crossref | GoogleScholarGoogle Scholar |

Wegner LH, Zimmermann U (1998) Simultaneous recording of xylem pressure and trans-root potential in roots of intact glycophytes using a novel xylem pressure probe technique. Plant, Cell & Environment 21, 849–865.
Simultaneous recording of xylem pressure and trans-root potential in roots of intact glycophytes using a novel xylem pressure probe technique.Crossref | GoogleScholarGoogle Scholar |

West JD, Peak D, Peterson JQ, Mott KA (2005) Dynamics of stomatal patches for a single surface of Xanthium strumarium L. leaves observed with fluorescence and thermal images. Plant, Cell & Environment 28, 633–641.
Dynamics of stomatal patches for a single surface of Xanthium strumarium L. leaves observed with fluorescence and thermal images.Crossref | GoogleScholarGoogle Scholar |

Westhoff M, Reuss R, Zimmermann D, Netzer Y, Gessner A, Geßner P, Zimmermann G, Wegner LH, Bamberg E, Schwartz A, Zimmermann U (2009) A non-invasive probe for online-monitoring of turgor pressure changes under field conditions. Plant Biology 11, 701–712.
A non-invasive probe for online-monitoring of turgor pressure changes under field conditions.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1MnitFeltQ%3D%3D&md5=b52de07cfc74536be48042991f8f3e2cCAS | 19689778PubMed |

Winter M, Koopmann B, Doll K, Karlovsky P, Kropf U, Schluter K, von Tiedemann A (2013) Mechanisms regulating grain contamination with trichothecenes translocated from the stem base of wheat (Triticum aestivum) infected with Fusarium culmorum. Phytopathology 103, 682–689.
Mechanisms regulating grain contamination with trichothecenes translocated from the stem base of wheat (Triticum aestivum) infected with Fusarium culmorum.Crossref | GoogleScholarGoogle Scholar | 23758328PubMed |

Zee S, O’brien T (1970) A special type of tracheary element associated with ‘xylem discontinuity’ in the floral axis of wheat. Australian Journal of Biological Sciences 23, 783–792.

Zhang J, Jia W, Yang J, Ismail AM (2006) Role of ABA in integrating plant responses to drought and salt stresses. Field Crops Research 97, 111–119.
Role of ABA in integrating plant responses to drought and salt stresses.Crossref | GoogleScholarGoogle Scholar |

Zimmermann MH (1983) ‘Xylem structure and the ascent of sap.’ (Springer-Verlag: Berlin)

Zimmermann U, Schneider H, Wegner LH, Haase A (2004) Water ascent in tall trees: does evolution of land plants rely on a highly metastable state? New Phytologist 162, 575–615.
Water ascent in tall trees: does evolution of land plants rely on a highly metastable state?Crossref | GoogleScholarGoogle Scholar |

Zimmermann D, Reuss R, Westhoff M, Gessner P, Bauer W, Bamberg E, Bentrup FW, Zimmermann U (2008) A novel, non-invasive, online-monitoring, versatile and easy plant-based probe for measuring leaf water status. Journal of Experimental Botany 59, 3157–3167.
A novel, non-invasive, online-monitoring, versatile and easy plant-based probe for measuring leaf water status.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXpslalsLk%3D&md5=28bd44b301997057b98aee27627c2fd1CAS | 18689442PubMed |

Zimmermann U, Rüger S, Shapira O, Westhoff M, Wegner LH, Reuss R, Gessner P, Zimmermann G, Israeli Y, Zhou A, Schwartz A, Bamberg E, Zimmermann D (2010) Effects of environmental parameters and irrigation on the turgor pressure of banana plants measured using the non-invasive, online monitoring leaf patch clamp pressure probe. Plant Biology 12, 424–436.
Effects of environmental parameters and irrigation on the turgor pressure of banana plants measured using the non-invasive, online monitoring leaf patch clamp pressure probe.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3czps1alsg%3D%3D&md5=463106bed0d91ddcdb8261f251bec153CAS | 20522178PubMed |

Zimmermann U, Bitter R, Marchiori P, Rüger S, Ehrenberger W, Sukhorukov V, Schüttler A, Ribeiro R (2013) A non-invasive plant-based probe for continuous monitoring of water stress in real time: a new tool for irrigation scheduling and deeper insight into drought and salinity stress physiology. Theoretical and Experimental Plant Physiology 25, 2–11.
A non-invasive plant-based probe for continuous monitoring of water stress in real time: a new tool for irrigation scheduling and deeper insight into drought and salinity stress physiology.Crossref | GoogleScholarGoogle Scholar |