Exploiting a fast neutron mutant genetic resource in Pisum sativum (pea) for functional genomics
Claire Domoney A , Maggie Knox A , Carol Moreau A , Mike Ambrose A , Sarah Palmer A B , Peter Smith B , Vangelis Christodoulou C , Peter G. Isaac C , Matthew Hegarty D , Tina Blackmore D , Martin Swain D and Noel Ellis D E F
+ Author Affiliations
- Author Affiliations
A Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
B Wherry and Sons Ltd, The Old School, High Street, Rippingale, Bourne, Lincolnshire PE10 0SR, UK.
C IDna Genetics Ltd, Norwich Research Park, Norwich NR4 7UH, UK.
D Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Ceredigion SY23 3EB, UK.
E Present address: CGIAR Research Program on Grain Legumes, c/o ICRISAT, Patancheru 502 324, Andhra Pradesh, India.
F Corresponding author. Email: noe2@aber.ac.uk
This paper originates from a presentation at the ‘VI International Conference on Legume Genetics and Genomics (ICLGG)’ Hyderabad, India, 2–7 October 2012.
Functional Plant Biology 40(12) 1261-1270 https://doi.org/10.1071/FP13147
Submitted: 19 May 2013 Accepted: 18 July 2013 Published: 28 August 2013
Journal Compilation © CSIRO Publishing 2013 Open Access CC BY-NC-ND
Abstract
A fast neutron (FN)-mutagenised population was generated in Pisum sativum L. (pea) to enable the identification and isolation of genes underlying traits and processes. Studies of several phenotypic traits have clearly demonstrated the utility of the resource by associating gene deletions with phenotype followed by functional tests exploiting additional mutant sources, from both induced and natural variant germplasm. For forward genetic screens, next generation sequencing methodologies provide an opportunity for identifying genes associated with deletions rapidly and systematically. The application of rapid reverse genetic screens of the fast neutron mutant pea population supports conclusions on the frequency of deletions based on phenotype alone. These studies also suggest that large deletions affecting one or more loci can be non-deleterious to the pea genome, yielding mutants that could not be obtained by other means. Deletion mutants affecting genes associated with seed metabolism and storage are providing unique opportunities to identify the products of complex and related gene families, and to study the downstream consequences of such deletions.
Additional keywords: fast neutron mutagenesis, genomic deletions, seed proteins.
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