Manipulation of methyl jasmonate esterase activity renders tomato more susceptible to Sclerotinia sclerotiorum
Simone Findling A , Agnes Fekete A , Heribert Warzecha B , Markus Krischke A , Hendrik Brandt A , Ernst Blume A , Martin J. Mueller A and Susanne Berger A C
+ Author Affiliations
- Author Affiliations
A Julius-von-Sachs-Institute for Biosciences, Pharm. Biology, Biocenter, University of Wuerzburg, Julius-von-Sachs-Platz 2, 97082 Wuerzburg, Germany.
B Present address: Technical University Darmstadt, Schnittspahnstr. 10, 64287 Darmstadt, Germany.
C Corresponding author. Email: berger@biozentrum.uni-wuerzburg.de
Functional Plant Biology 41(2) 133-143 https://doi.org/10.1071/FP13103
Submitted: 18 April 2013 Accepted: 24 July 2013 Published: 20 September 2013
Abstract
Jasmonic acid methyl ester has been discussed as a stress signal in plants. To investigate the relevance of reversible methylation of jasmonic acid, stress responses of transgenic tomato lines with altered expression and activity of methyl jasmonate esterase were analysed. No consistent changes in levels of methyl jasmonate, 12-oxo-phytodienoic acid, jasmonic acid, jasmonic acid isoleucine and expression of the jasmonate-responsive genes AOC and PINII between control line and RNAi as well as overexpressing lines were detectable under basal and wound-induced conditions. In contrast, reduction as well as enhancement of methyl jasmonate esterase activity resulted in increased susceptibility to the fungal pathogen Sclerotinia sclerotiorum despite higher levels of the hormonal active jasmonic acid isoleucine conjugate. Results suggest that methyl jasmonate esterase has a function in vivo in plant defence, which appears not to be related to its in vitro capacity to hydrolyse methyl jasmonate.
Additional keywords: methyl esterase, necrotrophic fungus.
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