Taxonomic resolution of actinorhizal Myrica species from Meghalaya (India) through nuclear rDNA sequence analyses
Mhathung Yanthan A , David Biate A and Arvind K. Misra A BA Department of Botany, North-Eastern Hill University, Shillong 793022, Meghalaya, India.
B Corresponding author. Email: arvindkmisra@nehu.ac.in
This paper originates from a presentation at the 16th International Meeting on Frankia and Actinorhizal Plants, Oporto, Portugal, 5–8 September 2010.
Functional Plant Biology 38(9) 738-746 https://doi.org/10.1071/FP10248
Submitted: 21 December 2010 Accepted: 20 June 2011 Published: 16 August 2011
Abstract
Three different morphological variants of genus Myrica L. (Myricaceae) are found in Meghalaya, India. There is a dispute regarding their classification. Some authors treat them as two separate species (Myrica nagi Thunb. and Myrica esculenta Buch.-Ham. ex D. Don) whereas others feel that M. nagi and M. esculenta are synonyms. In an effort to resolve this dispute, we investigated the nuclear rRNA genes segment of DNA of these variants utilising the amplicon restriction patterns, nucleotide sequences and secondary folding of the 5.8S rRNA. It is proposed that M. nagi and M. esculenta be treated as two separate species.
Additional keyword: nuclear ribosomal RNA genes.
References
Baird JR (1969) A taxonomic revision of the plant family Myricaceae of North America, North of Mexico. Dissertation, University of North Carolina, USA.Balakrishnan NP (1983) ‘Flora of Jowai. Vol. I.’ (Botanical Survey of India: Howrah, India)
Bor NL (1953) ‘Manual of Indian forest botany.’ (Oxford University Press: New Delhi)
Brummitt RK (1999) Proposals to conserve or reject. Report of the committee for spermatophyte. Taxon 48, 367–368.
Chauhan VS, Misra AK (2002) Development of molecular markers for early screening of Alnus nepalensis D. Don genotypes for the nitrogenase activity of actinorhizal root nodules. Molecular Genetics and Genomics 267, 303–312.
| Development of molecular markers for early screening of Alnus nepalensis D. Don genotypes for the nitrogenase activity of actinorhizal root nodules.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XlvFSjsLo%3D&md5=5fecb1c8aa967a8971a1fbad37501d41CAS |
Cronquist A (1988) ‘The evolution and classification of flowering plants.’ (New York Botanical Garden: New York)
Dhyani PP, Dhar U (1994) ‘Myrica esculenta box myrtle kaiphal. A promising underexploited tree crop of the Himalaya.’ (Bishen Singh Mahendra Pal Singh: Dehradun, India)
Don D (1825) Prodromus Florae Nepalensis, sive Enumeratio Vegetabilium, quae in Itinere per Nepaliam Proprie Dictam et Rgiones Conterminas, Ann. 1802–1803. Detexit atque legit D. D. Franciscus Hamilton, (olim Buchanan) M.D. London, 56.
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
| Confidence limits on phylogenies: an approach using the bootstrap.Crossref | GoogleScholarGoogle Scholar |
Haridasan K, Rao RR (1985) ‘Forest flora of Meghalaya. Vol. II. Caprifoliaceae to Salicaceae.’ (Bishen Singh Mahendra Pal Singh: Dehradun, India)
Huguet V, Gouy M, Normand P, Zimpfer JF, Fernandez MP (2005) Molecular phylogeny of Myricaceae: a re-examination of host–symbiont specificity. Molecular Phylogenetics and Evolution 34, 557–568.
| Molecular phylogeny of Myricaceae: a re-examination of host–symbiont specificity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXptlCiug%3D%3D&md5=5379a67dea1ea763c1d2902b085b3a55CAS |
Jaccard P (1908) Nouvelles researches sur la distribution florate. Bullettin Society Vaudoise Science Naturalle 44, 223–270.
Jamann S, Fernandez MP, Normand P (1993) Typing method for N2 fixing bacteria based on PCR-RFLP application to the characterization of Frankia strains. Molecular Ecology 2, 17–26.
| Typing method for N2 fixing bacteria based on PCR-RFLP application to the characterization of Frankia strains.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXkt1Wnu7k%3D&md5=63bc5794a9761a535aca6709ed4ce6a2CAS |
Khan A, Myrold DD, Misra AK (2007) Distribution of Frankia genotypes occupying Alnus nepalensis nodules with respect to altitude and soil characteristics in the Sikkim Himalayas. Physiologia Plantarum 130, 364–371.
| Distribution of Frankia genotypes occupying Alnus nepalensis nodules with respect to altitude and soil characteristics in the Sikkim Himalayas.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXns1OqurY%3D&md5=8c42dfa09c23ac821ca15af8a0d2e22dCAS |
Khan A, Myrold DD, Misra AK (2009) Molecular diversity of Frankia from root nodules of Hippophae salicifolia (D.Don) found in Sikkim. Indian Journal of Microbiology 49, 196–200.
| Molecular diversity of Frankia from root nodules of Hippophae salicifolia (D.Don) found in Sikkim.Crossref | GoogleScholarGoogle Scholar |
Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16, 111–120.
| A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3MXmtFSktg%3D%3D&md5=ea374ef794a7eafe49c668da052519a6CAS |
Li R, Chen Z, Lu A, Soltis DE, Soltis PS, Manos PS (2004) Phylogenetic relationships in Fagales based on DNA sequences from three genomes. International Journal of Plant Sciences 165, 311–324.
| Phylogenetic relationships in Fagales based on DNA sequences from three genomes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlvFCisL4%3D&md5=7e4e6687467212e77bf8634fca1dd292CAS |
Murray JA (1784) Systema Vegetabilium: secundum classes ordines genera species cum characteribus et differentiis. Editio decima quarta. Gottingae.
Navarro E, Bousquet J, Moiroud A, Munive A, Piou D, Normand P (2003) Molecular phylogeny of Alnus (Betulaceae), inferred from nuclear ribosomal DNA ITS sequences. Plant and Soil 254, 207–217.
| Molecular phylogeny of Alnus (Betulaceae), inferred from nuclear ribosomal DNA ITS sequences.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXlvVKht7c%3D&md5=fa048f7d23accaa8e1010877fec23f85CAS |
Parmar C, Kaushal MK (1982) Myrica nagi. In ‘Wild fruits of sub-Himalayan region’. p. 136. (Kalyani Publishers: New Delhi)
Qiu Y-L, Chase MW, Hoot SB, Conti E, Crane PR, Sytsma KJ, Parks CR (1998) Phylogenetics of the hamamelidae and their allies: parsimony analyses of nucleotide sequences of the plastid gene rbcL. International Journal of Plant Sciences 159, 891–905.
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406–425.
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain termination inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74, 5463–5467.
| DNA sequencing with chain termination inhibitors.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1cXhtlaru7Y%3D&md5=f0319e892361e3a5bcf182fc0e5859e8CAS |
Sporne KR (1975) ‘The morphology of angiosperms.’ (St Martin’s Press: New York)
Takhtajan A (1969) ‘Flowering plants: origin and dispersal.’ (Oliver and Boyd: Edinburgh)
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution
| MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.Crossref | GoogleScholarGoogle Scholar | in press.
Wen J, Zimmer EA (1996) Phylogeny and biogeography of Panax L. (the ginseng genus, Araliaceae): references from ITS sequences of nuclear ribosomal DNA. Molecular Phylogenetics and Evolution 6, 167–177.
| Phylogeny and biogeography of Panax L. (the ginseng genus, Araliaceae): references from ITS sequences of nuclear ribosomal DNA.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XmvFOjtLs%3D&md5=4422b27a7f73c58addfe09c5565a7604CAS |
Yap IV, Nelson RJ (1996) ‘WINBOOT: a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms. IRRI discussion paper series No. 14.’ pp. 1–22. (International Rice Research Institute: Manila, Philippines)