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Plant function and evolutionary biology
RESEARCH ARTICLE

Naturally occurring arbuscular mycorrhizal fungi can replace direct P uptake by wheat when roots cannot access added P fertiliser

Huiying Li A D , Sally E. Smith A , Kathy Ophel-Keller A B , Robert E. Holloway A C and F. Andrew Smith A
+ Author Affiliations
- Author Affiliations

A Soil and Land Systems, School of Earth and Environmental Sciences, Waite Campus, DX650636, The University of Adelaide, SA 5005, Australia.

B Plant and Soil Health, Plant Research Centre, South Australian Research and Development Institute, GPO Box 397, Adelaide, SA 5001, Australia.

C Minnipa Agricultural Centre, South Australian Research and Development Institute, PO Box 31, Minnipa, SA 5654, Australia.

D Corresponding author. Email: h.li@adelaide.edu.au

Functional Plant Biology 35(2) 124-130 https://doi.org/10.1071/FP07202
Submitted: 21 August 2007  Accepted: 22 January 2008   Published: 19 March 2008

Abstract

We investigated the roles of naturally occurring arbuscular mycorrhizal (AM) fungi in phosphorus (P) uptake by wheat (Triticum aestivum L.) in a calcareous, P-fixing soil. Plants grew in a main pot containing autoclaved soil (NM) or autoclaved soil mixed with non-autoclaved soil (to provide inoculum of naturally occurring AM fungi; AM). Granular (GP; monoammonium phosphate) or fluid (FP; ammonium polyphosphate) fertilisers were applied in small compartments (PCs) within a main pot, to which either roots plus hyphae (–Mesh) or hyphae only (+Mesh) had access. Controls received no additional P (NP). Inoculated plants were well colonised by AM fungi. AM growth depressions were observed in –Mesh treatments with NP and GP, but not with FP. Neither AM growth nor P responses were observed in +Mesh treatments. AM plants had much higher P uptake than NM plants, regardless of the P and mesh treatments. Total P uptake by NM plants increased with FP in –Mesh, but was unaffected by either form of P in the +Mesh treatments. Total P uptake by AM plants was similar between –Mesh and +Mesh treatments, regardless of applied P, showing that roots plus hyphae and hyphae alone have the same ability to obtain P. Thus, hyphae can take over the roles of roots in P uptake when roots are not able to access P sources.

Additional keywords: calcareous soils, fluid P, grain yield, granular P, mycorrhizal responses.


Acknowledgements

This work was supported by The South Australian Grains Industry Trust (SAGIT; Project no. UA1/05) and ARC-Linkage (Project no. LP0669161). We thank Robert Davidson for teaching us to use WinRHIZO, Colin Rivers and Rebecca Stonor for technical support, and Dot Brace (Minnipa Agricultural Centre, South Australian Research and Development Institute) for providing the soil. We are grateful to unknown reviewers for valuable comments on a draft of this paper.


References


Bertrand I, Holloway RE, Armstrong RD, McLaughlin MJ (2003) Chemical characteristics of phosphorus in alkaline soils from southern Australia. Australian Journal of Soil Research 41, 61–76.
Crossref | GoogleScholarGoogle Scholar | open url image1

Drew MC, Saker LR (1978) Nutrient supply and the growth of the seminal root system of barley. III. Compensatory increases in growth of lateral roots, and in rates of phosphate uptake, in response to a localized supply of phosphate. Journal of Experimental Botany 29, 435–451.
Crossref | GoogleScholarGoogle Scholar | open url image1

Graham JH, Abbott LK (2000) Wheat responses to aggressive and non-aggressive arbuscular mycorrhizal fungi. Plant and Soil 220, 207–218.
Crossref | GoogleScholarGoogle Scholar | open url image1

Hanson WC (1950) The photometric determination of phosphorus in fertilizers using the phosphovanado-molybdate complex. Journal of the Science of Food and Agriculture 1, 172–173.
Crossref | GoogleScholarGoogle Scholar | open url image1

Hetrick BAD, Wilson GWT, Todd TC (1996) Mycorrhizal response in wheat cultivars: relationship to phosphorus. Canadian Journal of Botany 74, 19–25.
Crossref | GoogleScholarGoogle Scholar | open url image1

Hettiarachchi GM, Lombi E, McLaughlin MJ, Chittleborough D, Self P (2006) Density changes around phosphorus granules and fluid bands in a calcareous soil. Soil Science Society of America Journal 70, 960–966.
Crossref | GoogleScholarGoogle Scholar | open url image1

Holloway RE, Bertrand I, Frischke AJ, Brace DM, McLaughlin MJ, Shepperd W (2001) Improving fertiliser efficiency on calcareous and alkaline soils with fluid sources of P, N and Zn. Plant and Soil 236, 209–219.
Crossref | GoogleScholarGoogle Scholar | open url image1

Jakobsen I, Abbott LK, Robson AD (1992) External hyphae of vesicular-arbuscular mycorrhizal fungi associated with Trifolium subterraneum L. 1. Spread of hyphae and phosphorus inflow into roots. New Phytologist 120, 371–380.
Crossref | GoogleScholarGoogle Scholar | open url image1

Koide RT (2000) Mycorrhizal symbiosis and plant reproduction. In ‘Arbuscular mycorrhizas: physiology and function’. (Eds Y Kapulnik, DDJ Douds) pp. 19–46. (Kluwer Academic Publishers: Dordrecht, The Netherlands)

Li HY (2005) Roles of mycorrhizal symbiosis in growth and phosphorus nutrition of wheat in a highly calcareous soil. PhD Thesis, The University of Adelaide.

Li HY, Zhu YG, Marschner P, Smith FA, Smith SE (2005) Wheat responses to arbuscular mycorrhizal fungi in a highly calcareous soil differ from those of clover, and change with plant development and P supply. Plant and Soil 277, 221–232.
Crossref | GoogleScholarGoogle Scholar | open url image1

Li HY, Smith SE, Holloway RE, Zhu YG, Smith FA (2006) Arbuscular mycorrhizal (AM) fungi contribute to phosphorus uptake by wheat grown in a phosphorus-fixing soil even in the absence of positive growth responses. New Phytologist 172, 536–543.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Lombi E, McLaughlin MJ, Johnston C, Armstrong RD, Holloway RE (2004) Mobility and lability of phosphorus from granular and fluid monoammonium phosphate differs in a calcareous soil. Soil Science Society of America Journal 68, 682–689. open url image1

Lombi E, Scheckel KG, Armstrong RD, Forrester S, Cutler JN, Paterson D (2006) Speciation and distribution of phosphorus in a fertilized soil: a synchrotron-based investigation. Soil Science Society of America Journal 70, 2038–2048.
Crossref | GoogleScholarGoogle Scholar | open url image1

Lu X, Koide RT (1991) Avena fatua L. seed and seedling nutrient dynamics as influenced by mycorrhizal infection of the maternal generation. Plant, Cell & Environment 14, 931–939.
Crossref | GoogleScholarGoogle Scholar | open url image1

McBeath TM, Armstrong RD, Lombi E, McLaughlin MJ, Holloway RE (2005) Responsiveness of wheat (Triticum aestivum) to liquid and granular phosphorus fertilisers in southern Australian soils. Australian Journal of Soil Research 43, 203–212.
Crossref | GoogleScholarGoogle Scholar | open url image1

McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swan JA (1990) A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytologist 115, 495–501.
Crossref | GoogleScholarGoogle Scholar | open url image1

Ravnskov S, Jakobsen I (1995) Functional compatibility in arbuscular mycorrhizas measured as hyphal P transport to the plant. New Phytologist 129, 611–618.
Crossref | GoogleScholarGoogle Scholar | open url image1

Read DJ (1984) The structure and function of the vegetative mycelium of mycorrhizal roots. In ‘The ecology and physiology of the fungal mycelium’. (Eds DH Jennings, ADM Rayner) pp. 215–240. (Cambridge University Press: Cambridge)

Regent-Instruments (1996) ‘WinRHIZO V3.9 reference.’ (Regent Instruments: Quebec, Canada)

Ryan MH, Graham JH (2002) Is there a role for arbuscular mycorrhizal fungi in production agriculture? Plant and Soil 244, 263–271.
Crossref | GoogleScholarGoogle Scholar | open url image1

Ryan MH, Norton RM, Kirkegaard JA, McCormick KM, Knights SE, Angus JF (2002) Increasing mycorrhizal colonisation does not improve growth and nutrition of wheat on Vertosols in south-eastern Australia. Australian Journal of Agricultural Research 53, 1173–1181.
Crossref | GoogleScholarGoogle Scholar | open url image1

Schweiger PF, Jakobsen I (1999) Direct measurement of arbuscular mycorrhizal phosphorus uptake into field-grown winter wheat. Agronomy Journal 91, 998–1002. open url image1

Smith SE , Read DJ (1997) ‘Mycorrhizal symbiosis.’ 2nd edn. (Academic Press: San Diego, CA)

Soil Survey Staff (1994) ‘Keys to soil taxonomy.’ 6th edn. (United States Department of Agriculture, Soil Conservation Service, Washington, DC)

Vierheilig H, Coughlan AP, Wyss U, Piche Y (1988) Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Applied and Environmental Microbiology 64, 5004–5007. open url image1

Zhu J, Kaeppler SM, Lynch JP (2005) Topsoil foraging and phosphorus acquisition efficiency in maize (Zea mays). Functional Plant Biology 32, 749–763.
Crossref | GoogleScholarGoogle Scholar | open url image1

Zhu YG, Smith SE (2001) Seed phosphorus (P) content affects growth, and P uptake of wheat plants and their association with arbuscular mycorrhizal (AM) fungi. Plant and Soil 231, 105–112.
Crossref | GoogleScholarGoogle Scholar | open url image1

Zhu YG, Smith SE, Barritt AR, Smith FA (2001) Phosphorus (P) efficiencies and mycorrhizal responsiveness of old and modern wheat cultivars. Plant and Soil 237, 249–255.
Crossref | GoogleScholarGoogle Scholar | open url image1