Boron uptake by the root cortex symplast of tomato and pea plants: evidence for low-boron-induced active transport
Jasna Savic A C , Miroslav Nikolic B D , Slaven Prodanovic A and Volker Römheld CA Faculty of Agriculture, University of Belgrade, 11080 Zemun-Belgrade, Serbia.
B Department of Natural Resources and Environmental Sciences, Centre for Multidisciplinary Studies, University of Belgrade, 11030 Belgrade, Serbia.
C Institute of Plant Nutrition (330), University of Hohenheim, 70593 Stuttgart, Germany.
D Corresponding author. Email: mnikolic@cms.bg.ac.yu
Functional Plant Biology 34(12) 1130-1136 https://doi.org/10.1071/FP07175
Submitted: 16 July 2007 Accepted: 23 October 2007 Published: 27 November 2007
Abstract
The objective of this research was to test the hypothesis of the existence of an active boron (B) uptake into the cortical cells induced by low B supply. The uptake of B was characterised in two tomato (Lycopersicon esculentum Mill.) genotypes: B-efficient FER and B-inefficient mutant T3238. In addition, pea (Pisum sativum L.) was used as an anatomically appropriate model for obtaining intact root cortex. Time course uptake studies in tomato indicate that the B-inefficient mutant was defective by the absence of an active low-B-induced uptake system in the cortex. Pea roots showed up to 10-fold higher accumulation of B into the cortex symplast at low (0.5 µm) external B supply in comparison to adequate B (10 µm) supply. Also, low-B-induced uptake of B was strongly inhibited by 2,4-dinitrophenol, indicating a metabolic energy-derived active component of B uptake at low external supply. Uptake of B by the cortical cells of tomato and pea plants appears to be a combination of both passive and active components, with a passive component prevailing at higher external B. An active component of B uptake suppressed by either adequate or high B supply might indicate a downregulation of plasma membrane-associated B transporter(s) in root cortical cells.
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