Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Effects of subcutaneous administration of caffeine on the physiometabolic profile of low-birthweight neonate piglets

H. Orozco-Gregorio A B , H. Bonilla-Jaime B , D. Mota-Rojas C G , M. E. Trujillo-Ortega D , P. Roldan-Santiago C , R. Martínez-Rodríguez E , F. Borderas-Tordesillas C , S. Flores-Peinado F , P. Mora-Medina F and R. Ramírez-Necoechea C

A Doctoral Program in Biological Sciences and Health, Universidad Autónoma Metropolitana, Iztapalapa/Xochimilco, México D.F., México.

B Universidad Autónoma Metropolitana, Iztapalapa, Department of Reproductive Biology, México D.F., México.

C Universidad Autónoma Metropolitana, Xochimilco, Stress Physiology and Farm Animal Welfare, Department of Animal Production and Agriculture, Calzada del Hueso 1100, Col. Villa Quietud, México D.F., 04960, México.

D Universidad Nacional Autónoma de México, Department of Animal Medicine and Production, Swine, Faculty of Veterinary and Animal Production, México D.F., México.

E Universidad Nacional Autónoma de México, Center for Teaching, Research and Extension in Swine Production (CEIEPP), Faculty of Veterinary and Animal Production, Jilotepec, Estado de México, México.

F Universidad Nacional Autónoma de México, Department of Livestock Sciences, Facultad de Estudios Superiores Cuautitlán, Cuautitlán Izcalli, Estado de México.

G Corresponding author. Email: dmota100@yahoo.com.mx

Animal Production Science 52(11) 981-990 http://dx.doi.org/10.1071/AN11199
Submitted: 20 September 2011  Accepted: 1 May 2012   Published: 25 July 2012

Abstract

Caffeine administration has been used to stimulate respiration and reverse metabolic alterations in high-birthweight piglets that suffer neonatal asphyxia. However, its effect has not been evaluated on low-birthweight neonates. This study determined the effect of subcutaneously administering caffeine on the physiometabolic profile of neonate piglets with low birthweight and neonatal asphyxia. A 35-mg subcutaneous dose of caffeine, or an equivalent placebo, was randomly administered at 0 and 24 h to neonate piglets (n = 120) diagnosed with intrapartum asphyxia and classified as low-(LW) or high weight (HW) in a 2 by 2 experimental design. Blood samples were obtained at 0, 2 and 24 h postpartum. At 2 h, blood glucose concentrations diminished in the animals in both groups that were treated with caffeine (P < 0.005). Of those animals, only the ones in HW showed increased glucose concentrations by 24 h. At 0 h, group LW exhibited the highest levels of pCO2 (P < 0.005). In contrast, from 2 to 24 h only the neonates in group HW treated with caffeine reached the levels of pCO2 and pO2 characteristic of non-asphyxiated piglets. At the conclusion of the experiment, the weight of the piglets in group LW that were treated with caffeine was less than that of those that received the placebo. In contrast, the weight of the piglets in group HW that were treated with caffeine was 19% higher than that of those which received the placebo (P < 0.005). In this study, administering two doses of 35 mg of caffeine to LW neonate piglets significantly accelerated their energy expenditure, thus reducing their weight gain at 8 days of life; in contrast, the physiometabolic profile of the HW neonate piglets improved and they showed greater weight gain.

Additional keywords: lactate, methylxanthine.


References

Bauer J, Maier K, Linderkamp P, Hentschel R (2001) Effect of caffeine on oxygen consumption and metabolic rate in very low birth weight infants with idiopathic apnea. Pediatrics 107, 660–663.
Effect of caffeine on oxygen consumption and metabolic rate in very low birth weight infants with idiopathic apnea.CrossRef | 1:STN:280:DC%2BD3M3ptVersA%3D%3D&md5=62dd5dd758c5bd490c79c034930cb8d5CAS | open url image1

Beaulieu AD, Aalhus JL, Williams NH, Patience JF (2010) Impact of piglet birth weight, birth order, and litter size on subsequent growth. Journal of Animal Science 88, 2767–2778.
Impact of piglet birth weight, birth order, and litter size on subsequent growth.CrossRef | 1:CAS:528:DC%2BC3cXpvVOrt7o%3D&md5=87b2b4a48906baef0582efaef335cb28CAS | open url image1

Benowitz NL (1990) Clinical pharmacology of caffeine. Annual Review of Medicine 41, 277–288.
Clinical pharmacology of caffeine.CrossRef | 1:CAS:528:DyaK3cXktVSjs74%3D&md5=8694b79c3c7654794d40fcad5cea6c1aCAS | open url image1

Caceres L, Bilkei G, McGil M, Pena FJ (2001) The effect of levamisole on the preweaning performance of lightweight born piglets. Medicina Veterinaria 18, 435–438.

Carter AJ, O’connor WT, Carter MJ, Ungerstedt U (1995) Caffeine enhances acetylcholine release in the hippocampus in vivo by a selective interaction with adenosine A1 receptors. Journal of Pharmacology and Experimental Therapeutics 237, 637–642.

Chardon K, Bach V, Telliez F, Cardot V, Tourneux P, Leke A, Libert J (2004) Effect of caffeine on peripheral chemopreceptor activity in premature neonates: interaction with sleep stages. Journal of Applied Physiology 96, 2161–2166.
Effect of caffeine on peripheral chemopreceptor activity in premature neonates: interaction with sleep stages.CrossRef | 1:CAS:528:DC%2BD2cXltV2msLc%3D&md5=2113e5f33b6b8a3c91d31a074f39b9adCAS | open url image1

Edwards AV, Silver M (1969) The effect of asphyxia on the plasma glucose concentration in new-born calves. Biologia Neonatorum 14, 1–7.
The effect of asphyxia on the plasma glucose concentration in new-born calves.CrossRef | 1:STN:280:DyaE3c%2Fjs1eksQ%3D%3D&md5=98f6fc515285a7a1e9660de13b0ca77cCAS | open url image1

Elliot JI, Lodge GA (1977) Body compostion and glycogen reserves in the neonatal pig during the first 96 hours post-partum. Canadian Journal of Animal Science 57, 141–150.
Body compostion and glycogen reserves in the neonatal pig during the first 96 hours post-partum.CrossRef | 1:CAS:528:DyaE2sXktVCnsb8%3D&md5=d3e2dc2c447e476b364c082d8c1ff641CAS | open url image1

England DC (1974) Husbandry components in prenatal and perinatal development in swine. Journal of Animal Science 38, 1045–1049.

Ergenekon E, Dalgiç N, Aksoy E, Koç E, Atalay Y (2001) Caffeine intoxication in a premature neonate. Paediatric Anaesthesia 11, 737–739.
Caffeine intoxication in a premature neonate.CrossRef | 1:STN:280:DC%2BD3MnksVCnug%3D%3D&md5=19cd5fbbeb5aa535312c42aabe6e109aCAS | open url image1

González-Lozano M, Mota-Rojas D, Velázquez-Armenta EY, Nava-Ocampo AA, Hernández-González R, Becerril-Herrera M, Trujillo-Ortega ME, Alonso-Spilsbury M (2009a) Obstetric and fetal outcomes in dystocic and eutocic sows to an injection of exogenous oxytocin during farrowing. The Canadian Veterinary Journal. La Revue Veterinaire Canadienne 50, 1273–1277.

González-Lozano M, Trujillo-Ortega ME, Becerril-Herrera M, Alonso-Spilsbury M, Ramírez-Necoechea R, Hernández-González R, Mota-Rojas D (2009b) Effects of oxytocin on critical blood variables from dystocic sows. Veterinaria (México) 40, 231–245.

Handel SE, Stickland NC (1987) Muscle cellularity and birth weight. Animal Production 44, 311–317.
Muscle cellularity and birth weight.CrossRef | open url image1

Herpin P, Le Dividich J, Claude HJ, Fillaut M, De Marco F, Bertin R (1996) Effects of the level of asphyxia during delivery on viability at birth and early postnatal vitality of newborn pigs. Journal of Animal Science 74, 2067–2075.

Herpin P, Damon M, Le Dividich J (2002) Development of thermoregulation and neonatal survival in pigs. Livestock Production Science 78, 25–45.
Development of thermoregulation and neonatal survival in pigs.CrossRef | open url image1

Hoy S, Lutter C, Wahner M, Puppe B (1994) Influence of birth weight on the early postnatal vitality of piglets. DTW. Deutsche Tierarztliche Wochenschrift 101, 393–396.

Jezkova D, Smrckova M (1990) Variations of glucosaemia and lactacidemia in pregnant sows, fetuses and in sows and piglets till the 10th day after delivery. Veterinarni Medicina 35, 613–620.

Knight RJ, Bowmer CJ, Yates MS (1993) The diuretic action of 8-cyclopentyl-1,3-dipropylxanthine, a selective A1 adenosine receptor antagonist. British Journal of Pharmacology 109, 271–277.

Litten JC, Drury PC, Corson AM, Lean IJ, Clarke L (2003) The influence of piglet birth weight on physical and behavioural development in early life. Biology of the Neonate 84, 311–318.
The influence of piglet birth weight on physical and behavioural development in early life.CrossRef | 1:STN:280:DC%2BD3srhsVSqtQ%3D%3D&md5=cf52aa9b0098ad7a61334b394db657bfCAS | open url image1

Marchant JN, Broom DM, Corning S (2001) The influence of sow behavior on piglet mortality due to crushing in an open farrowing system. Animal Science (Penicuik, Scotland) 72, 19–28.

Martínez-Rodríguez R, Mota-Rojas D, Trujillo-Ortega ME, Orozco-Gregorio H, Hernández-González R, Roldán-Santiago P, Mora-Medina P, Alonso SM, Rosales AM, Ramírez-Necoechea R (2011) Physiological response to hypoxia in piglets of different birth weight. Italian Journal of Animal Science 10, 250–253.
Physiological response to hypoxia in piglets of different birth weight.CrossRef | open url image1

Massey LK, Whiting SJ (1993) Caffeine, urinary calcium, calcium metabolism and bone. Journal of Nutrition 123, 1611–1614.

Massey LK, Wise KJ (1992) Impact of gender and age on urinary water and mineral excretion responses to acute caffeine doses. Nutrition Research (New York, N.Y.) 12, 605–612.
Impact of gender and age on urinary water and mineral excretion responses to acute caffeine doses.CrossRef | 1:CAS:528:DyaK38XltlSisLk%3D&md5=48fe49fd70a1931a849cc655aa7aebf7CAS | open url image1

McCarter FD, James JH, Luchette FA, Wang L, Friend LA, King J, Evans JM, George MA, Fischer JE (2001) Adrenergic blockade reduces skeletal muscle glycolysis and Na+, K+-ATPase activity during hemorrhage. Journal of Surgical Research 99, 235–244.
Adrenergic blockade reduces skeletal muscle glycolysis and Na+, K+-ATPase activity during hemorrhage.CrossRef | 1:CAS:528:DC%2BD3MXlsVSjtr8%3D&md5=9ed15f05f8bc76584932c6625b50b707CAS | open url image1

Mota-Rojas D, Martínez-Burnes J, Trujillo-Ortega ME, Alonso-Spilsbury M, Ramírez-Necoechea R, López-Mayagoitia A (2002) Effect of oxytocin treatment in sows on umbilical cord morphology and meconium staining and neonatal mortality of piglets. American Journal of Veterinary Research 63, 1571–1574.
Effect of oxytocin treatment in sows on umbilical cord morphology and meconium staining and neonatal mortality of piglets.CrossRef | 1:CAS:528:DC%2BD38XovFart70%3D&md5=b739b43ef8787fe70ec1486fb3df51caCAS | open url image1

Mota-Rojas D, Martínez-Burnes J, Trujillo ME, Lopez A, Rosales AM, Ramirez R, Orozco H, Merino A, Alonso-Spilsbury M (2005a) Uterine and fetal asphyxia monitoring in parturient sows treated with oxytocin. Animal Reproduction Science 86, 131–141.
Uterine and fetal asphyxia monitoring in parturient sows treated with oxytocin.CrossRef | 1:CAS:528:DC%2BD2MXhsVOhur0%3D&md5=d567e86c5a76f41c5e9ed3e8345b8950CAS | open url image1

Mota-Rojas D, Nava-Ocampo A, Trujillo-Ortega ME, Velázquez-Armenta Y, Ramírez-Necoechea R, Martínez-Burnes J, Alonso-Spilsbury M (2005b) Dose minimization study of oxytocin in early labor in sows: uterine activity and fetal outcome. Reproductive Toxicology (Elmsford, N.Y.) 20, 255–259.
Dose minimization study of oxytocin in early labor in sows: uterine activity and fetal outcome.CrossRef | 1:CAS:528:DC%2BD2MXkt1Oitro%3D&md5=1c11649a66d938ae669a4f4108fd06d6CAS | open url image1

Mota-Rojas D, Martínez-Burnes J, Trujillo-Ortega ME, López A, Rosales AM, Ramírez-Necoechea R, Orozco H, Merino A, Alonso-Spilsbury M (2005c) The effects of Vetrabutin clorhidrate and oxytocin on stillbirth rate and asphyxia in swine. Theriogenology 64, 1889–1897.
The effects of Vetrabutin clorhidrate and oxytocin on stillbirth rate and asphyxia in swine.CrossRef | 1:CAS:528:DC%2BD2MXht1GitrrN&md5=2adfbade0e9316eb69ce1d1bc9f6452cCAS | open url image1

Mota-Rojas D, Martínez-Burnes J, Alonso-Spilsbury M, López A, Ramírez-Necoechea R, Trujillo-Ortega ME, Medina-Hernández FJ, de la Cruz NI, Albores-Torres V, Gallegos-Sagredo R (2006) Meconium staining of the skin and meconium aspiration in porcine intrapartum stillbirths. Livestock Science 102, 155–162.
Meconium staining of the skin and meconium aspiration in porcine intrapartum stillbirths.CrossRef | open url image1

Mota-Rojas D, Orozco-Gregorio H, Villanueva-Garcia D, Bonilla-Jaime H, Suarez-Bonilla X, Hernández-González R, Roldan-Santiago P, Trujillo-Ortega ME (2011) Foetal and neonatal energy metabolism in pigs and humans: a review. Veterinarni Medicina 56, 215–225.

Mota-Rojas D, Villanueva-García D, Hernández-González R, Roldan-Santiago P, Martínez-Rodríguez R, Mora-Medina P, González-Meneses B, Sánchez-Hernández M, Trujillo-Ortega ME (2012) Assessment of the vitality of the newborn: an overview. Scientific Research and Essays 7, 712–718.

Odehnalová S, Vinkler A, Novák P, Drábek J (2008) The dynamics of changes in selected parameters in relation to different air temperature in the farrowing house for sows. Czech Journal of Animal Science 53, 195–203.

Orozco-Gregorio H, Mota-Rojas D, Alonso-Spilsbury M, Alonso-Spilsbury M, Olmos-Hernández A, Ramírez-Necoechea R, Velazquez-Armenta EY, Nava-Ocampo AA, Hernández-González R, Trujillo-Ortega ME, Villanueva-García D (2008) Short-term neurophysiologic consequences of intrapartum asphyxia in piglets born by spontaneous parturition. The International Journal of Neuroscience 118, 1299–1315.
Short-term neurophysiologic consequences of intrapartum asphyxia in piglets born by spontaneous parturition.CrossRef | 1:CAS:528:DC%2BD1cXpslKnsL8%3D&md5=85c53ce5be0f01ffbc695d9653760deeCAS | open url image1

Orozco-Gregorio H, Mota-Rojas D, Bonilla-Jaime H, Trujillo-Ortega ME, Becerril-Herrera M, Hernández-González R, Villanueva-García D (2010) Effects of administration of caffeine on metabolic variables in neonatal pigs with peripartum asphyxia. American Journal of Veterinary Research 71, 1214–1219.
Effects of administration of caffeine on metabolic variables in neonatal pigs with peripartum asphyxia.CrossRef | 1:CAS:528:DC%2BC3cXhsFSrsL3N&md5=a4347a24b071bc3ac9c71924c2344405CAS | open url image1

Quiniou N, Dagorn J, Gaudre D (2002) Variation of piglets’ birth weight and consequences on subsequent performance. Livestock Production Science 78, 63–70.
Variation of piglets’ birth weight and consequences on subsequent performance.CrossRef | open url image1

Randall GCB (1979) Studies on the effect of acute asphyxia on the fetal pig in utero. Biology of the Neonate 36, 63–69.
Studies on the effect of acute asphyxia on the fetal pig in utero.CrossRef | 1:CAS:528:DyaE1MXktlOis7c%3D&md5=59bdb8d7871428f5314b5f76c40a5101CAS | open url image1

Rootwelt V, Reksen O, Framstad T (2012) Production traits of litters in 2 crossbred Duroc pig lines. Journal of Animal Science 90, 152–158.
Production traits of litters in 2 crossbred Duroc pig lines.CrossRef | 1:CAS:528:DC%2BC38XnvFOisQ%3D%3D&md5=ca4c0ff9ec27f8711e4a1c2bd5050431CAS | open url image1

Schmidt B, Roberts RS, Davis P, Doyle LW, Barrington KJ, Ohlsson A, Solimano A, Tin W (2006) Caffeine therapy for apnea of prematurity. The New England Journal of Medicine 354, 2112–2121.
Caffeine therapy for apnea of prematurity.CrossRef | 1:CAS:528:DC%2BD28XkslCku7Y%3D&md5=13f3c54c8feeed692a0f2a84e1743656CAS | open url image1

Sherwin CM, Christiansen SB, Duncan IJ, Erhard HW, Lay DC, Mench JA, O’Connor CE, Petherick JC (2003) Guidelines for the ethical use of animals in applied ethology studies. Applied Animal Behaviour Science 81, 291–305.
Guidelines for the ethical use of animals in applied ethology studies.CrossRef | open url image1

Steel GDR, Torrie HJ (1986) ‘Comparaciones multiples. Bioestadística principios y procedimientos.’ (McGraw-Hill-Interamericana: Bogotá, Columbia)

Sun D, Samuelson LC, Yang T, Huang Y, Paliege A, Saunders T, Briggs J, Schnermann J (2001) Mediation of tubuloglomerular feedback by adenosine: evidence from mice lacking adenosine 1 receptors. Proceedings of the National Academy of Sciences of the United States of America 98, 9983–9988.
Mediation of tubuloglomerular feedback by adenosine: evidence from mice lacking adenosine 1 receptors.CrossRef | 1:CAS:528:DC%2BD3MXmtlCmtrc%3D&md5=981c0fcda97272782e4389c9354924f7CAS | open url image1

Thomson S, Bao D, Deng A, Vallon V (2000) Adenosine formed by 5′-nucleotidase mediates tubuloglomerular feedback. Journal of Clinical Investigation 106, 289–298.
Adenosine formed by 5′-nucleotidase mediates tubuloglomerular feedback.CrossRef | 1:CAS:528:DC%2BD3cXkvFWgs7w%3D&md5=798ef2b04014d1830c7812bba7c283e1CAS | open url image1

Trujillo-Ortega ME, Mota-Rojas D, Olmos-Hernández A, Alonso-Spilsbury M, González M, Orozco H, Ramírez-Necoechea R, Nava-Ocampo AA (2007) A study of piglets born by spontaneous parturition under uncontrolled conditions: could this be a naturalistic model for the study of intrapartum asphyxia? Acta Biomedica 78, 29–35.

Trujillo-Ortega ME, Mota-Rojas D, Juárez O, Villanueva-García D, Becerril-Herrera M, Hernández-González R, Alonso-Spilsbury M, Martínez-Rodríguez R, Ramírez-Necoechea R (2011) Porcine neonates failing vitality score: physio-metabolic profile and latency to first teat contact. Czech Journal of Animal Science 56, 499–508.

Tsang RC, Chen IW, Friedman MA, Chen I (1973) Neonatal parathyroid function: role of gestational age and postnatal age. Journal of Pediatrics 83, 728–738.
Neonatal parathyroid function: role of gestational age and postnatal age.CrossRef | 1:STN:280:DyaE2c%2FgsFWitQ%3D%3D&md5=c70ba9c52200924f4fae83a0ae4e9c9fCAS | open url image1

van Buren M, Bijlsma JA, Boer P, van Rijn HJ, Koomans HA (2011) Natriuretic and hypotensive effect of adenosine-1 blockade in essential hypertension. Hypertension 22, 728–734.

Warnes DM, Seamark RF, Ballard FJ (1977) The appearance of gluconeogenesis at birth in sheep: activation of the pathway associated with blood oxygenation. Biochemical Journal 162, 627–634.

Wilcox CS, Welch WJ, Schreiner GF, Belardinelli L (1999) Natriuretic and diuretic actions of a highly selective adenosine A1 receptor antagonist. Journal of the American Society of Nephrology 10, 714–720.

Zaleski HM, Hacker RR (1993) Variables related to the progress of parturition and probability of stillbirth in swine. Canadian Veterinary Journal 34, 109–113.

Zanardo V, Dani C, Trevisanuto D, Meneghetti S, Guglielmi A, Zacchello G, Cantarutti F (1995) Methylxanthines increase renal calcium excretion in preterm infants. Biology of the Neonate 68, 169–174.
Methylxanthines increase renal calcium excretion in preterm infants.CrossRef | 1:CAS:528:DyaK2MXpvFyltbY%3D&md5=90aa05655df3d4aa96d8cd59680bd91cCAS | open url image1



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