Dimethylsulfoxide and conjugated linoleic acids affect bovine embryo development in vitro
Hanna Stinshoff A C E , Sandra Wilkening A , Ana Hanstedt A B , Heinrich Bollwein A D and Christine Wrenzycki A B C
+ Author Affiliations
- Author Affiliations
A Clinic for Cattle, University of Veterinary Medicine, Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
B Unit for Reproductive Medicine, University of Veterinary Medicine, Hannover, Foundation, Bünteweg 15, 30559 Hannover, Germany.
C Present address: Clinic for Veterinary Obstetrics, Gynecology and Andrology, Frankfurter Str. 106, 35392 Giessen, Germany.
D Present address: Clinic for Reproductive Medicine, Large Animal Department, Vetsuisse-Fakultät Universität Zürich, Winterthurerstrasse 260, 8057 Zürich, Switzerland.
E Corresponding author. Email: hanna.stinshoff@vetmed.uni-giessen.de
Reproduction, Fertility and Development 26(4) 502-510 https://doi.org/10.1071/RD12372
Submitted: 15 November 2012 Accepted: 13 March 2013 Published: 11 April 2013
Abstract
Conjugated linoleic acids (CLA) are employed to overcome the bovine periparturitional negative energy balance. Especially of interest are trans10,cis12 -linoleic acid (t10c12-CLA) and cis9,trans11-linoleic acid (c9t11-CLA). Their impact on embryonic development, though, is not clear. Here, effects of both above-mentioned CLA on bovine in vitro-produced embryos were assessed. Zygotes (n = 2098) were allocated to one of seven groups: cultured with 50 or 100 µM of either c9t11-CLA or t10c12-CLA, with 14 or 28 mM DMSO or without supplement (control). Messenger RNA analysis of target gene transcripts (IGF1R, IGFBP2, IGFBP4, CPT2, ACAA1, ACAA2, FASN, SCD) via RT-qPCR was performed in single blastocysts. Cleavage rates did not differ, whereas development rates were decreased in both t10c12-supplemented groups in comparison to the unsupplemented group (31.7% ± 2.2 control vs 20.2% ± 2.0 50 µM t10c12 vs 21.0% ± 2.8 100 µM t10c12). Compared with the unsupplemented group, SCD was expressed at a lower level in embryos cultured with 50 µM c9t11-CLA. The relative amount of several transcripts was increased in embryos cultured with 14 mM DMSO in comparison to those that developed in the presence of 50 µM t10c12-CLA (IGFBP2, ACAA1, CPT2, FASN, SCD) or 50 µM c9t11-CLA (IGF1R, IGFBP2, ACAA1, CPT2, FASN, SCD). The molecular analyses show that CLA influence embryonic fat metabolism.
Additional keywords: CLA, DMSO, gene expression, high yielding dairy cows, IVP, negative energy balance.
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