Cryotolerance of in vitro-produced porcine blastocysts is improved when using glucose instead of pyruvate and lactate during the first 2 days of embryo culture
M. Castillo-Martín A C , M. Yeste B , R. Morató B , T. Mogas B and S. Bonet A
+ Author Affiliations
- Author Affiliations
A Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, Campus Montilivi, E-17071 Girona, Spain.
B Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Spain.
C Corresponding author. Email: miriam.castillo@udg.edu
Reproduction, Fertility and Development 25(5) 737-745 https://doi.org/10.1071/RD12117
Submitted: 13 April 2012 Accepted: 12 June 2012 Published: 8 August 2012
Abstract
The objective of the present study was to determine the effects of replacing glucose with pyruvate and lactate during the first 48 h of in vitro culture (IVC) in NCSU-23 medium on embryo development, embryo quality and survival of porcine blastocysts after vitrification. To this end, in vitro-produced (IVP) porcine oocytes were cultured with either glucose for 6 days (IVC-Glu) or pyruvate–lactate from Day 0 to Day 2 and then with glucose until Day 6 (IVC-PyrLac). Blastocysts were vitrified on Day 6 using the Cryotop device and, after warming, survival rate and the apoptosis index were evaluated after 24 h incubation in NCSU-23 medium. No significant differences were observed between IVC-Glu and IVC-PyrLac in terms of cleavage rate, blastocyst yield, total number of cells per blastocyst or the apoptosis index (1.82 ± 0.75% vs 3.18 ± 0.88%, respectively) of non-vitrified embryos. However, a significant increase was seen in hatching/hatched blastocysts in the IVC-PyrLac compared with IVC-Glu treatment group (12.71 ± 1.20% vs 3.54 ± 0.47%, respectively). Regardless of treatment, vitrification impaired the survival rate and the apoptosis index. When comparing both treatments after warming, the percentage of apoptotic cells was significantly higher for blastocysts in the IVC-PyrLac compared with IVC-Glu group (18.55 ± 3.49% vs 9.12 ± 2.17%, respectively). In conclusion, under the conditions of the present study, replacement of glucose with pyruvate–lactate during the first 48 h of culture resulted in a lower cryotolerance of IVP porcine embryos.
Additional keywords: energy substrate, vitrification.
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