Bone morphogenetic protein-6 (BMP-6): mRNA expression and effect on steroidogenesis during in vitro maturation of porcine cumulus oocyte complexes
S. Ebeling A C , D. Töpfer A , J. M. Weitzel B and B. Meinecke A
+ Author Affiliations
- Author Affiliations
A Department of Reproductive Biology, University of Veterinary Medicine Hannover, Foundation, Germany.
B Unit of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.
C Corresponding author. Email: silja.ebeling@tiho-hannover.de
Reproduction, Fertility and Development 23(8) 1034-1042 https://doi.org/10.1071/RD11027
Submitted: 24 January 2011 Accepted: 6 May 2011 Published: 22 September 2011
Abstract
Oocyte secreted factors (OSFs) have emerged as important factors for follicular development. The present study investigated the effect of the potential OSF bone morphogenic protein (BMP)-6 on steroidogenesis in porcine cumulus oocyte complexes during in vitro maturation. Cumulus oocyte complexes (COCs), cumulus complexes (CCs) without oocytes and CCs with supplemented BMP-6 were cultured for 0, 5, 26 or 46 h. BMP-6 transcripts were detected in oocytes and cumulus cells at all time points. In both cell types the mRNA expression was most intense after 5 h, and decreased during further maturation. After 26 and 46 h of culture, CCs secreted significantly less 17β-estradiol than COCs. This effect was reversed by adding BMP-6 to CCs cultures. In addition, a down-regulation of Cyp19A1, the rate-limiting enzyme of 17β-estradiol synthesis, was detected in CC cultures after 5 h. As seen for 17β-estradiol secretion, the addition of BMP-6 caused a significant increase in Cyp19A1 mRNA levels after 5, 26 and 46 h of culture. Progesterone secretion and transcripts of steroidogenic marker proteins StAR and 3β-HSD were not affected considerably by oocyte removal or addition of BMP-6. Furthermore, BMP-6 did not affect the activity of the mitogen-activated protein kinase. The results indicated that BMP-6 is a potential OSF and is involved in the prevention of premature luteinisation in cumulus cells via enhancing 17β-estradiol synthesis.
Additional keywords: 17β-estradiol, mitogen-activated protein kinase (MAPK), oocyte secreted factor (OSF), progesterone.
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