Vitrification of bovine blastocysts pretreated with sublethal hydrostatic pressure stress: evaluation of post-thaw in vitro development and gene expression
E. Siqueira Filho A B , E. S. Caixeta B , C. Pribenszky C D , M. Molnar C , A. Horvath C , A. Harnos C , M. M. Franco A and R. Rumpf A
+ Author Affiliations
- Author Affiliations
A Embrapa Genetic Research and Biotechnology, Laboratory of Animal Reproduction, Parque Estação Biológica W5 Norte Final, Brasília 70770-900, DF, Brazil.
B University of Brasília, School of Agriculture and Veterinary Medicine, Brasília 70770-900, DF, Brazil.
C Faculty of Veterinary Science, Szent István University, 1078 Budapest, István u. 2, Hungary.
D Corresponding author. Email: pribenszky.csaba@aotk.szie.hu
Reproduction, Fertility and Development 23(4) 585-590 https://doi.org/10.1071/RD10203
Submitted: 19 August 2010 Accepted: 11 December 2010 Published: 3 May 2011
Abstract
Sublethal stress treatment has been reported to enhance gametes’ performance in subsequent procedures, such as cryopreservation. The aim of the present study was to evaluate the effect of different equilibration times between the termination of a sublethal hydrostatic pressure (HP) stress treatment and the initiation of vitrification on the post-thaw survival, continued in vitro development, hatching rate and gene expression of selected candidate genes of in vitro-produced (IVP) expanded bovine blastocysts. Day 7 IVP blastocysts were subjected to 600 bar pressure for 60 min at 32°C. Immediately after pressure treatment (HP0h) or after 1 or 2 h incubation (HP1h and HP2h groups, respectively), embryos were either vitrified and warmed using the open pulled straw method, followed by 72 h in vitro culture or were stored at –80°C until gene expression analysis. Re-expansion and hatching rates after vitrification–warming were significantly (P < 0.05) higher in the HP0h (88 and 76%, respectively) and HP1h (90 and 75%, respectively) groups than in the untreated (82 and 63%, respectively) and HP2h groups (79 and 70%, respectively). Moreover, the HP1h group showed further improvement in the speed of re-expansion and resumption of normal in vitro development. Cumulative analysis of all genes (SC4MOL, HSP1A1A, SOD2 and GPX4) revealed a similar pattern of expression, with a tendency for peak transcript abundance 1 h after HP treatment. Application of HP stress treatment was found to be efficient in increasing the in vitro developmental competence of vitrified bovine embryos.
Additional keywords: cryopreservation, cryotolerance, preconditioning, stress.
References
Aertsen, A., de Spiegeleer, P., Vanoirbeek, K., Lavilla, M., and Michiels, C. W. (2005). Induction of oxidative stress by high hydrostatic pressure in Escherichia coli. Appl. Environ. Microbiol. 71, 2226–2231.| Induction of oxidative stress by high hydrostatic pressure in Escherichia coli.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXktFKmtrs%3D&md5=361b2a99ee23c321a2ae2d59c1a56831CAS | 15870304PubMed |
Agca, Y., Monson, R. L., Northey, D. L., Abas Mazni, O., and Rutledge, J. J. (1994). Post-thaw survival and pregnancy rates of in vitro produced bovine embryos after vitrification. Theriogenology 41, 154. [Abstract]
Alexandre, H., Ansanay-Galeote, V., and Dequin, S. (2001). Global gene expression during short-term ethanol stress in Saccharomyces cerevisiae. FEBS Lett. 498, 98–103.
| Global gene expression during short-term ethanol stress in Saccharomyces cerevisiae.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXktVKqs7g%3D&md5=a2d10299238fe9e6496bc9ffda382705CAS | 11389906PubMed |
Bogliolo, P. L., Ariu, F., Uccheddu, S., Strina, A., Rosati, I., Zedda, M. T., and Ledda, S. (2009). High hydrostatic pressure treatment improves the quality of in vitro-produced ovine blastocysts. Reprod. Fertil. Dev. 22, 202. [Abstract]
Diller, K. R. (2006). Stress protein expression kinetics. Annu. Rev. Biomed. Eng. 8, 403–424.
| Stress protein expression kinetics.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xpt1CltLc%3D&md5=8a3ea744f1ad81c358612c34e5a2e63dCAS | 16834562PubMed |
Dinnyes, A., Polgar, Z., Pribenszky, C., and Pirity, M. K. (2010). Improved embryoid body cryopreservation and cardiomyocyte differentiation following high hydrostatic pressure treatment. In ‘Proceedings of the 1st International Congress on Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue and Organs, Valencia, Spain, 2010’. (ComtecMed: Israel.)
Du, Y., Pribenszky, C., Molnár, M., Zhang, X., Yang, H., Kuwayama, M., Pedersen, A. M., Villemoes, K., Bolund, L., and Vajta, G. (2008a). High hydrostatic pressure (HHP): a new way to improve in vitro developmental competence of porcine matured oocytes after vitrification. Reproduction 135, 13–17.
| High hydrostatic pressure (HHP): a new way to improve in vitro developmental competence of porcine matured oocytes after vitrification.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhs1CksL8%3D&md5=40791d16b93fdb5869c2b24e049634e6CAS | 18159079PubMed |
Du, Y., Lin, L., Schmidt, M., Bøgh, I. B., Kragh, P. M., Sørensen, C. B., Li, J., Purup, S., Pribenszky, C., Molnár, M., Kuwayama, M., Zhang, X., Yang, H., Bolund, L., and Vajta, G. (2008b). High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival. Cloning Stem Cells 10, 325–330.
| High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtVOmu7zK&md5=b506b8174c8acf205ea12b3eddf32e98CAS | 18479211PubMed |
Fernandes, P. M. (2005). How does yeast respond to pressure. Braz. J. Med. Biol. Res. 39, 1239–1245.
Fernandes, P. M., Domitrovic, T., Kao, C. M., and Kurtenbach, E. (2004). Genomic expression pattern in Saccharomyces cerevisiae cells in response to high hydrostatic pressure. FEBS Lett. 556, 153–160.
| Genomic expression pattern in Saccharomyces cerevisiae cells in response to high hydrostatic pressure.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXosl2h&md5=cf618de3d263caaf9b280bb32f1e6d38CAS | 14706843PubMed |
Holm, P., Booth, P. J., Schmidt, M. H., Greve, T., and Callesen, H. (1999). High bovine blastocyst development in a static in vitro production system using sofaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology 52, 683–700.
| High bovine blastocyst development in a static in vitro production system using sofaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3c7pvVGnsw%3D%3D&md5=26633a11a012ea179b9a1f80585e2f19CAS | 10734366PubMed |
Huang, S. H., Pribenszky, C., Kuo, Y. H., Teng, S. H., Chen, Y. H., Chung, M. T., and Chiu, Y. F. (2009). Hydrostatic pressure pre-treatment affects the protein profile of boar sperm before and after freezing–thawing. Anim. Reprod. Sci. 112, 136–149.
| Hydrostatic pressure pre-treatment affects the protein profile of boar sperm before and after freezing–thawing.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXivVWktLY%3D&md5=2a49c99e47c4c0f934903021e7bfd878CAS | 18538515PubMed |
Kaarniranta, K., Elo, M., Sironen, R., Lammi, M. J., Goldring, M. B., Eriksson, J. E., Sistonen, L., and Helminen, H. J. (1998). Hsp 70 accumulation in chondrocytic cells exposed to high continuous hydrostatic pressure coincides with mRNA stabilization rather than transcriptional activation. Proc. Natl Acad. Sci. USA 95, 2319–2324.
| Hsp 70 accumulation in chondrocytic cells exposed to high continuous hydrostatic pressure coincides with mRNA stabilization rather than transcriptional activation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXhsleit7g%3D&md5=4f40a662c3dac4c649a5f9a8847693e2CAS |
Kuo, Y. H., Pribenszky, C., and Huang, S. Y. (2008). Higher litter size is achieved by the insemination of high hydrostatic pressure-treated frozen–thawed boar semen. Theriogenology 70, 1395. [Abstract]
Ledda, S., Bogliolo, L., Ariu, F., Bebbere, D., Uccheddu, S., Strina, A., Pintus, E., and Nieddu, S. (2010). High hydrostatic pressure treatment prior to vitrification improves the re-expansion speed and quality of in vitro-produced ovine embryos. In ‘Proceedings of The 1st International Congress on Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue and Organs, Valencia, Spain, 2010’. (ComtecMed: Israel.)
Parrish, J. J., Krogenaes, A., and Susko-Parrish, J. L. (1995). Effect of bovine sperm separation by either swin-up and percoll method on success of in vitro fertilization and early embryonic development. Theriogenology 44, 859–869.
| Effect of bovine sperm separation by either swin-up and percoll method on success of in vitro fertilization and early embryonic development.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD28zgtVGgug%3D%3D&md5=689eef0f0257d52abff008c998071eb2CAS | 16727781PubMed |
Pribenszky, C., Molnar, M., Cseh, S., and Solti, L. (2005). Improving post-thaw survival of cryopreserved mouse blastocysts by hydrostatic pressure challenge. Anim. Reprod. Sci. 87, 143–150.
| Improving post-thaw survival of cryopreserved mouse blastocysts by hydrostatic pressure challenge.Crossref | GoogleScholarGoogle Scholar | 15885447PubMed |
Pribenszky, C., Molnar, M., Horvath, A., Harnos, A., and Szenci, O. (2006). Hydrostatic pressure induced increase in post-thaw motility of frozen boar spermatozoa. Reprod. Fertil. Dev. 18, 162–163. [Abstract]
Pribenszky, C., Molnár, M., Horváth, A., Kútvölgyi, G., Harnos, A., Szenci, O., Dengg, J., and Lederer, J. (2007). Improved post-thaw motility, viability and fertility are achieved by hydrostatic pressure treated bull semen. Reprod. Fertil. Dev. 19, 181–182. [Abstract]
Pribenszky, C., Du, Y., Molnár, M., Harnos, A., and Vajta, G. (2008a). Increased stress tolerance of matured pig oocyte by high hydrostatic pressure impulse. Anim. Reprod. Sci. 106, 200–207.
| Increased stress tolerance of matured pig oocyte by high hydrostatic pressure impulse.Crossref | GoogleScholarGoogle Scholar | 18329829PubMed |
Pribenszky, C., Siquiera, E., Molnár, M., and Rumpf, R. (2008b). Improved post-warming developmental competence of open pulled straw-vitrified in vitro-produced bovine blastocysts by sublethal hydrostatic pressure pre-treatment. Reprod. Fertil. Dev. 20, 125. [Abstract]
Pribenszky, C., Du, Y., Molnar, M., and Vajta, G. (2008c). Sublethal stress on porcine oocytes enhances the efficacy of ART procedures. Hum. Reprod. 23, 161. [Abstract]
Robertson, I., and Nelson, R. (1998). ‘Manual of the International Embryo Transfer Society.’ 3rd edn. (International Embryo Transfer Society: Savoy, IL.)
Sahara, T., Goda, T., and Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. J. Biol. Chem. 277, 50 015–50 021.
| Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XpsFakurc%3D&md5=4bc4d88b9a5660a9ada4242a220845f2CAS |
Vajta, G., Holm, P., Kuwayama, M., Booth, P. J., Jacobsen, H., Greve, T., and Callesen, H. (1998a). Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol. Reprod. Dev. 51, 53–58.
| Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXltVGrs7g%3D&md5=9852074c4677c138223caab73aa4db9bCAS | 9712317PubMed |
Vajta, G., Kuwayama, M., Booth, P. J., Holm, P., Greve, T., and Callesen, H. (1998b). Open pulled straw (OPS) vitrification of cattle oocytes. Theriogenology 49, 176. [Abstract]
