Prolonged storage of epididymal spermatozoa does not affect their capacity to fertilise in vitro-matured domestic cat (Felis catus) oocytes when using ICSI
J. Ringleb A B , R. Waurich A , G. Wibbelt A , W. J. Streich A and K. Jewgenow A
+ Author Affiliations
- Author Affiliations
A Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke.Str. 17; 10315 Berlin, Germany.
B Corresponding author. Email: ringleb@izw-berlin.de
Reproduction, Fertility and Development 23(6) 818-825 https://doi.org/10.1071/RD10192
Submitted: 5 August 2010 Accepted: 21 March 2011 Published: 13 July 2011
Abstract
The impact of different storage conditions of epididymal spermatozoa (including prolonged storage, cryopreservation and freeze-drying) on their fertilisation capacity was tested using intracytoplasmic sperm injection (ICSI). This kind of information is urgently needed when applying assisted reproductive technology to endangered felids in zoos. In particular, the utilisation of epididymal spermatozoa of castrated or deceased felids often requires time-consuming transportation and is therefore susceptible to loss of gamete quality. Sperm cells were stored at 4°C for up to 72 h followed by cryopreservation or freeze-drying. Thawed motile and immotile spermatozoa were used for ICSI and the embryo cleavage rate was assessed 36 h after injection. A significant impact on the fertilisation rate of oocytes could only be detected when using immotile thawed or rehydrated spermatozoa. Cryopreservation or storage at 4°C showed no influence. The simulation of transport conditions using domestic cat spermatozoa revealed that in vitro production of felid embryos with gametes from euthanised individuals is possible if testes are stored cool and arrive at the laboratory within 72 h. An essential prerequisite is the application of ICSI to achieve fertilisation even with single motile spermatozoa. Additional cryopreservation of spermatozoa after transportation is possible and will allow the establishment of a sperm bank for felids.
Additional keywords: Felidae, gamete banking.
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