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Vertebrate reproductive science and technology
RESEARCH ARTICLE

Pregnancy obtained in a late gestational mare by in vitro embryo production

Lino Fernando Campos-Chillon https://orcid.org/0000-0003-3223-8059 A D , Jan Martin B and Joy L. Altermatt A C
+ Author Affiliations
- Author Affiliations

A Animal Science Department, California Polytechnic State University, 1 Grand Ave. San Luis Obispo, CA 93407, USA.

B Jan Martin Equine Management, PO Box 475, Buellton, CA 93427, USA.

C Veterinary Reproduction Innovations, APC, PO Box 4712, San Luis Obispo, CA 93403, USA.

D Corresponding author. Email: lcamposc@calpoly.edu

Reproduction, Fertility and Development 31(12) 1926-1929 https://doi.org/10.1071/RD19305
Submitted: 6 June 2019  Accepted: 2 September 2019   Published: 2 October 2019

Abstract

Recently, the demand for in vitro embryo production in the horse has increased worldwide. Most clinical transvaginal ultrasound-guided ovum pick-up (OPU) procedures are performed in non-pregnant donor mares, and few experimental studies have described in vitro embryo production from oocytes of pregnant donors 21–150 days in gestation. This report discusses OPU, follicular growth and in vitro embryo production in a pregnant mare during late gestation.

Additional keywords: ICSI, ovum pick up, follicle.


References

Altermatt, J. L., Suh, T. K., Stokes, J. E., and Carnevale, E. M. (2009). Effects of age and equine follicle-stimulating hormone (eFSH) on collection and viability of equine oocytes assessed by morphology and developmental competency after intracytoplasmic sperm injection (ICSI). Reprod. Fertil. Dev. 21, 615–623.
Effects of age and equine follicle-stimulating hormone (eFSH) on collection and viability of equine oocytes assessed by morphology and developmental competency after intracytoplasmic sperm injection (ICSI).Crossref | GoogleScholarGoogle Scholar | 19383268PubMed |

Amoroso, E. C., Hancock, J. L., and Rowlands, I. W. (1948). Ovarian activity in the pregnant mare. Nature 161, 355–356.
Ovarian activity in the pregnant mare.Crossref | GoogleScholarGoogle Scholar | 18908583PubMed |

Campos-Chillon, F., Farmerie, T. A., Bouma, G. J., Clay, C. M., and Carnevale, E. M. (2015). Effects of aging on gene expression and mitochondrial DNA in the equine oocyte and follicle cells. Reprod. Fertil. Dev. 27, 925–933.
Effects of aging on gene expression and mitochondrial DNA in the equine oocyte and follicle cells.Crossref | GoogleScholarGoogle Scholar | 25786490PubMed |

Campos-Chillon, L. F., Owen, C. M., and Altermatt, J. L. (2019). Equine and bovine oocyte maturation in a novel medium without CO2 gas phase. J. Equine Vet. Sci. 73, 51–55.
Equine and bovine oocyte maturation in a novel medium without CO2 gas phase.Crossref | GoogleScholarGoogle Scholar |

Carnevale, E. M., and Ginther, O. J. (1995). Defective oocytes as a cause of subfertility in old mares. Biol. Reprod. 52, 209–214.
Defective oocytes as a cause of subfertility in old mares.Crossref | GoogleScholarGoogle Scholar |

Choi, Y.-H., Velez, I. C., Macías-García, B., Riera, F. L., Ballard, C. S., and Hinrichs, K. (2016). Effect of clinically-related factors on in vitro blastocyst development after equine ICSI. Theriogenology 85, 1289–1296.
Effect of clinically-related factors on in vitro blastocyst development after equine ICSI.Crossref | GoogleScholarGoogle Scholar | 26777560PubMed |

Claes, A., Cuervo-Arango, J., Colleoni, S., Lazzari, G., Galli, C., and Stout, T. A. (2018). Production and sex ratio of foals after transfer of frozen–thawed in vitro produced embryos. J. Equine Vet. Sci. 66, 446.
Production and sex ratio of foals after transfer of frozen–thawed in vitro produced embryos.Crossref | GoogleScholarGoogle Scholar |

Cochran, R., Meintjes, M., Reggio, B., Hylan, D., Carter, J., Pinto, C., Paccamonti, D., Graff, K. J., and Godke, R. A. (2000). Production of live foals from sperm-injected oocytes harvested from pregnant mares. J. Reprod. Fertil. Suppl. 56, 503–512.

Cuervo-Arango, J., Claes, A. N., and Stout, T. A. (2019). A retrospective comparison of the efficiency of different assisted reproductive techniques in the horse, emphasizing the impact of maternal age. Theriogenology 132, 36–44.
A retrospective comparison of the efficiency of different assisted reproductive techniques in the horse, emphasizing the impact of maternal age.Crossref | GoogleScholarGoogle Scholar | 30986613PubMed |

Dowsett, K. F., and Pattie, W. A. (1982). Characteristics and fertility of stallion semen. J. Reprod. Fertil. Suppl. 32, 1–8.
| 6962847PubMed |

Foss, R. (2017). Equine ICSI, a private practice perspective: expectations, discussion, and thoughts. In ‘Society for Theriogenology Conference’. Clinical Theriogenology 9, 385–388.

Franz, L. C., Squires, E. L., O’Donovan, M. K., Scott, T. J., and Carnevale, E. C. (2001). Collection and in vitro maturation of equine oocytes from estrus, diestrus and pregnant mares. J. Equine Vet. Sci. 21, 26–32.
Collection and in vitro maturation of equine oocytes from estrus, diestrus and pregnant mares.Crossref | GoogleScholarGoogle Scholar |

Galli, C., Colleoni, S., Duchi, R., Lagutina, I., and Lazzari, G. (2007). Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture, cryopreservation and somatic cell nuclear transfer. Anim. Reprod. Sci. 98, 39–55.
Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture, cryopreservation and somatic cell nuclear transfer.Crossref | GoogleScholarGoogle Scholar | 17101246PubMed |

Gonzalez-Castro, R. A., and Carnevale, E. M. (2018). Association of equine sperm population parameters with outcome of intracytoplasmic sperm injections. Theriogenology 119, 114–120.
Association of equine sperm population parameters with outcome of intracytoplasmic sperm injections.Crossref | GoogleScholarGoogle Scholar | 30006126PubMed |

Henneke, D. R., Potter, G. D., Kreider, J. L., and Yeates, B. F. (1983). Relationship between condition score, physical measurements and body fat percentage in mares. Equine Vet. J. 15, 371–372.
Relationship between condition score, physical measurements and body fat percentage in mares.Crossref | GoogleScholarGoogle Scholar | 6641685PubMed |

Hinrichs, K. (2016). Equine oocyte recovery and ICSI in clinical practice: what can I expect? In ‘Society for Theriogenology Conference’, Clinical Theriogenology. 8, 285–290.

Li, L. Y., Meintjes, M., Graff, K. J., Paul, J. B., Denniston, R. S., and Godke, R. A. (1995). In vitro fertilization and development of in vitro-matured oocytes aspirated from pregnant mares. Biol. Reprod. 52, 309–317.
In vitro fertilization and development of in vitro-matured oocytes aspirated from pregnant mares.Crossref | GoogleScholarGoogle Scholar |

Meintjes, M., Bellow, M. S., Paul, J. B., Broussard, J. R., Li, L. Y., Paccamonti, D., Eilts, B. E., and Godke, R. A. (1995). Transvaginal ultrasound-guided oocyte retrieval from cyclic and pregnant horse and pony mares for in vitro fertilization. Biol. Reprod. 52, 281–292.
Transvaginal ultrasound-guided oocyte retrieval from cyclic and pregnant horse and pony mares for in vitro fertilization.Crossref | GoogleScholarGoogle Scholar |

Meintjes, M., Graff, K. J., Paccamonti, D., Eilts, B. E., Paul, J. B., Thompson, D. L., Kearney, M. T., and Godke, R. A. (1997). Effects of follicular aspiration and flushing, and the genotype of the fetus on circulating progesterone levels during pregnancy in the mare. Equine Vet. J. Suppl. 29, 25–32.

Morris, L. H. A. (2018). The development of in vitro embryo production in the horse. Equine Vet. J , .
The development of in vitro embryo production in the horse.Crossref | GoogleScholarGoogle Scholar |

Murase, H., Endo, Y., Tsuchiya, T., Kotoyori, Y., Shikichi, M., Ito, K., Sato, F., and Nambo, Y. (2014). Ultrasonographic evaluation of equine fetal growth throughout gestation in normal mares using a convex transducer. J. Vet. Med. Sci. 76, 947–953.
Ultrasonographic evaluation of equine fetal growth throughout gestation in normal mares using a convex transducer.Crossref | GoogleScholarGoogle Scholar | 24662520PubMed |

Owen, C. M., Barcelo-Fimbres, M., Altermatt, J. L., and Campos-Chillon, L. F. (2017). Survival of Holstein in vitro-produced embryos cultured in novel synthetic oviductal fluid media (scf1) and dehydrated prior to cryopreservation. Reprod. Fertil. Dev. 29, 129–130.
Survival of Holstein in vitro-produced embryos cultured in novel synthetic oviductal fluid media (scf1) and dehydrated prior to cryopreservation.Crossref | GoogleScholarGoogle Scholar |

Parrish, J. J., Susko-Parrish, J. L., and First, N. L. (1989). Capacitation of bovine sperm by heparin: inhibitory effect of glucose and role of intracellular pH. Biol. Reprod. 41, 683–699.
Capacitation of bovine sperm by heparin: inhibitory effect of glucose and role of intracellular pH.Crossref | GoogleScholarGoogle Scholar | 2620077PubMed |

Purcell, S. H., Seidel, G. E., McCue, P. M., and Squires, E. L. (2007). Aspiration of oocytes from transitional, cycling, and pregnant mares. Anim. Reprod. Sci. 100, 291–300.
Aspiration of oocytes from transitional, cycling, and pregnant mares.Crossref | GoogleScholarGoogle Scholar | 16938415PubMed |

Rambags, B. P. B., van Boxtel, D. C. J., Tharasanit, T., Lenstra, J. A., Colenbrander, B., and Stout, T. A. E. (2014). Advancing maternal age predisposes to mitochondrial damage and loss during maturation of equine oocytes in vitro. Theriogenology 81, 959–965.
Advancing maternal age predisposes to mitochondrial damage and loss during maturation of equine oocytes in vitro.Crossref | GoogleScholarGoogle Scholar |

Rizzo, M., Ducheyne, K. D., Deelen, C., Beitsma, M., Cristarella, S., Quartuccio, M., Stout, T. A. E., and Ruijter‐Villani, M. (2019). Advanced mare age impairs the ability of in vitro-matured oocytes to correctly align chromosomes on the metaphase plate. Equine Vet. J. 51, 252–257.
Advanced mare age impairs the ability of in vitro-matured oocytes to correctly align chromosomes on the metaphase plate.Crossref | GoogleScholarGoogle Scholar | 30025174PubMed |

Ruggeri, E., DeLuca, K. F., Galli, C., Lazzari, G., DeLuca, J. G., and Carnevale, E. M. (2015). Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after intracytoplasmic sperm injection. Reprod. Fertil. Dev. 27, 944–956.
Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after intracytoplasmic sperm injection.Crossref | GoogleScholarGoogle Scholar | 25798646PubMed |

Salgado, R. M., Brom-de-Luna, J. G., Resende, H. L., Canesin, H. S., and Hinrichs, K. (2018). Lower blastocyst quality after conventional vs. Piezo ICSI in the horse reflects delayed sperm component remodeling and oocyte activation. J. Assist. Reprod. Genet. 35, 825–840.
Lower blastocyst quality after conventional vs. Piezo ICSI in the horse reflects delayed sperm component remodeling and oocyte activation.Crossref | GoogleScholarGoogle Scholar | 29637506PubMed |