Cortical Intracellular Electrical Potential in Roots of Unstressed and Stressed Sunflower Seedlings. I. Dependence on Water Status

Abstract

The intracellular electrical potential (E_c) was measured with a microelectrode in cortical cells of intact roots of sunflower (Helianthus annuus) seedlings subjected to differing levels of water stress and illumination. Water stress was rapidly imposed and relieved by lowering and raising the level of nutrient solution in a tank which contained all the seedling roots except the lateral root in which E_c was measured. The base value of E_c for unstressed, illuminated seedlings was –120 mV. When placed in the dark, a small reversible depolarisation of approximately 10 mV could be measured in roots of seedlings grown under low levels of radiation but was not evident in seedlings grown under high levels of radiation. The imposition of and relief from extreme water stress (leaves and apex severely wilted) resulted in a reversible depolarisation of approximately 50 mV. The effect of water stress was greatly reduced by the presence of sucrose and glucose in the nutrient solution perfusing the 30 mm portion of the lateral root in which E_c was measured. It appears likely that the variation in potential was mediated by a reduction in the supply of photosynthate to the roots.