Grapevine varieties exhibiting differences in stomatal response to water deficit
Joaquim M. Costa A B D E , Maria F. Ortuño B C D , Carlos M. Lopes A and Maria M. Chaves A B
+ Author Affiliations
- Author Affiliations
A Centro de Botânica Aplicada à Agricultura, Instituto Superior de Agronomia – UTL, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
B Laboratório de Ecofisiologia Molecular, Instituto de Tecnologia Química e Biológica -UNL, Apartado 127, 2780-901 Oeiras, Portugal.
C Present address: Centro de Edafologia y Biologia Aplicada del Segura (CEBAS-CSIC), PO Box 164, 30100 Murcia, Spain.
D These authors contributed equally to this work.
E Corresponding author. Email: miguelc@itqb.unl.pt
Functional Plant Biology 39(3) 179-189 https://doi.org/10.1071/FP11156
Submitted: 15 July 2011 Accepted: 17 January 2012 Published: 2 March 2012
Abstract
Knowledge on variety traits and physiological responses to stress is still scarce in Vitis vinifera L., limiting the optimisation of irrigation and breeding for high water use efficiency. We have characterised five grapevine varieties using thermal imaging, leaf gas exchange, leaf morphology and carbon isotope composition. Plants of the varieties Aragonez, Trincadeira, Cabernet Sauvignon, Syrah and Touriga Nacional were grown in field conditions. Two experiments were performed. In Experiment I (2006), vines of Aragonez and Trincadeira were either well irrigated (WI, 80% ETc), non-irrigated but rain fed (NI) or subjected to regulated deficit irrigation (RDI, 40% ETc) and studied along the summer season. In Experiment II (2006 and 2007), vines of the five varieties were subjected to RDI (30–40% ETc) and studied at veraison. In Experiment I, leaf temperature (Tleaf) correlated negatively with stomatal conductance (gs) and leaf water potential (Ψpd). The inverse relationship between gs and Tleaf was highly significant in the afternoon. In Experiment II, the different genotypes showed different Tleaf for similar Ψpd. Stomatal density did not correlate with gs suggesting that varieties have different stomatal control. Our results show that combined measurements of canopy temperature and Ψpd can aid in better understanding of stomatal regulation in different grapevine varieties. Such variation in stomatal regulation should be taken into account in determining irrigation strategies.
Additional keywords: genotypes, stomatal conductance to water vapour, thermal imaging, Vitis vinifera, water stress.
References
Alchanatis V, Cohen Y, Cohen S, Möller M, Sprinstin M, Meron M, Tsipris J, Saranga Y, Sela E (2010) Evaluation of different approaches for estimating and mapping crop water status in cotton with thermal imaging. Precision Agriculture 11, 27–41.| Evaluation of different approaches for estimating and mapping crop water status in cotton with thermal imaging.Crossref | GoogleScholarGoogle Scholar |
Araus JL, Slafer GA, Royo C, Serret MD (2008) Breeding for yield potential and stress adaptation in cereals. Critical Reviews in Plant Sciences 27, 377–412.
| Breeding for yield potential and stress adaptation in cereals.Crossref | GoogleScholarGoogle Scholar |
Ben-Gal A, Agam N, Alchanatis V, Cohen Y, Yermiyahu U, Zipori I, Presnov E, Sprintsin M, Dag A (2009) Evaluating water stress in irrigated olives: correlation of soil water status, tree water status, and thermal imagery. Irrigation Science 27, 367–376.
| Evaluating water stress in irrigated olives: correlation of soil water status, tree water status, and thermal imagery.Crossref | GoogleScholarGoogle Scholar |
Bota J, Flexas J, Medrano H (2001) Genetic variability of photosynthesis and water use in Balearic grapevine cultivars. Annals of Applied Biology 138, 353–361.
| Genetic variability of photosynthesis and water use in Balearic grapevine cultivars.Crossref | GoogleScholarGoogle Scholar |
Chaerle L, Van der Straeten D (2000) Imaging techniques and the early detection of plant stress. Trends in Plant Science 5, 495–501.
| Imaging techniques and the early detection of plant stress.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3M%2FmtFSmug%3D%3D&md5=b1e61e30b47b8ff2883e77caa7fd9aceCAS |
Chaves MM (1991) Effects of water deficits on carbon assimilation. Journal of Experimental Botany 42, 1–16.
| Effects of water deficits on carbon assimilation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXhtFyntrw%3D&md5=766e211eaa83aca3df818da24a7545ddCAS |
Chaves MM, Rodrigues ML (1987) Photosynthesis and water relations of grapevines growing in Portugal response to environmental factors. In ‘Plant response to stress – functional analysis in Mediterranean ecosystems’. (Eds JD Tenhunen, FM Catarino, OL Lange, WC Oechel) pp. 379–390. (Springer-Verlag: Berlin)
Chaves MM, Tenhunen JD, Harley P, Lange OL (1987) Gas exchange studies in two Portuguese grapevine cultivars. Physiologia Plantarum 70, 639–647.
| Gas exchange studies in two Portuguese grapevine cultivars.Crossref | GoogleScholarGoogle Scholar |
Chaves MM, Santos TP, Souza CR, Ortuno MF, Rodrigues ML, Lopes CM, Maroco JP, Pereira JS (2007) Deficit irrigation in grapevine improves water-use efficiency while controlling vigour and production quality. Annals of Applied Biology 150, 237–252.
| Deficit irrigation in grapevine improves water-use efficiency while controlling vigour and production quality.Crossref | GoogleScholarGoogle Scholar |
Chaves MM, Zarrouk O, Francisco R, Costa JM, Santos T, Regalado AP, Rodrigues ML, Lopes CM (2010) Grapevine under deficit irrigation – hints from physiological and molecular data. Annals of Botany 105, 661–676.
| Grapevine under deficit irrigation – hints from physiological and molecular data.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3c3ovFGnsg%3D%3D&md5=846d9053fad60ff872755d1c783864cbCAS |
Cifre J, Bota J, Escalona JM, Medrano H, Flexas J (2005) Physiological tools for irrigation scheduling in grapevine (Vitis vinifera L.). An open gate to improve water-use efficiency? Agriculture Ecosystems & Environment 106, 159–170.
| Physiological tools for irrigation scheduling in grapevine (Vitis vinifera L.). An open gate to improve water-use efficiency?Crossref | GoogleScholarGoogle Scholar |
Cornic G (2000) Drought stress inhibits photosynthesis by decreasing stomatal aperture – not affecting ATP synthesis. Trends in Plant Science 5, 187–188.
| Drought stress inhibits photosynthesis by decreasing stomatal aperture – not affecting ATP synthesis.Crossref | GoogleScholarGoogle Scholar |
Costa JM, Ortuño MF, Chaves MM (2007) Deficit irrigation as a strategy to save water: physiology and potential application to horticulture. Journal of Integrative Plant Biology 49, 1421–1434.
| Deficit irrigation as a strategy to save water: physiology and potential application to horticulture.Crossref | GoogleScholarGoogle Scholar |
Costa JM, Grant OM, Chaves MM (2010) Use of thermal imaging in viticulture: current application and future prospects. In ‘Methodologies and results in grapevine research’. (Eds S Delrot, H Medrano, E Or, L Bavaresco, S Grando) pp. 135–150. (Springer: Dordrecht, The Netherlands)
COTR (2007) Centro Operativo e de Tecnologia de Regadio. Available at http://www.cotr.pt [Verified 9 February 2012]
de Souza CR, Maroco JP, Santos TP, Rodrigues ML, Lopes C, Pereira JS, Chaves MM (2005) Control of stomatal aperture and carbon uptake by deficit irrigation in two grapevine cultivars. Agriculture Ecosystems & Environment 106, 261–274.
| Control of stomatal aperture and carbon uptake by deficit irrigation in two grapevine cultivars.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhslehtLY%3D&md5=b42d8503666d7a23f1e07db7d4df69b3CAS |
Escalona JM, Flexas J, Medrano H (1999) Stomatal and non-stomatal limitations of photosynthesis under water stress in field-grown grapevines. Australian Journal of Plant Physiology 26, 421–433.
| Stomatal and non-stomatal limitations of photosynthesis under water stress in field-grown grapevines.Crossref | GoogleScholarGoogle Scholar |
FAO (1998) Crop evapotranspiration. (Eds RG Allen, LS Pereira, D Raes, M Smith). Irrigation and Drainage, Paper No. 56, FAO, Rome.
Flexas J, Escalona JM, Medrano H (1999) Water stress induces different levels of photosynthesis and electron transport rate regulation in grapevines. Plant, Cell & Environment 22, 39–48.
| Water stress induces different levels of photosynthesis and electron transport rate regulation in grapevines.Crossref | GoogleScholarGoogle Scholar |
Flexas J, Bota J, Escalona JM, Sampol B, Medrano H (2002) Effects of drought on photosynthesis in grapevines under field conditions. An evaluation of stomatal and mesophyll limitations. Functional Plant Biology 29, 461–471.
| Effects of drought on photosynthesis in grapevines under field conditions. An evaluation of stomatal and mesophyll limitations.Crossref | GoogleScholarGoogle Scholar |
Flexas J, Bota J, Loreto F, Cornic G, Sharkey TD (2004) Diffusive and metabolic limitations to photosynthesis under drought and salinity in C3 plants. Plant Biology 6, 269–279.
| Diffusive and metabolic limitations to photosynthesis under drought and salinity in C3 plants.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2c3ksVOlug%3D%3D&md5=9b9fece11d4e3f407332971891ab2c33CAS |
Flexas J, Galmés J, Gallé A, Gulias J, Pou A, Ribas-Carbo A, Tomás A, Medrano H (2010) Improving water use efficiency in grapevines: potential physiological targets for biotechnological improvement. Australian Journal of Grape and Wine Research 16, 106–121.
| Improving water use efficiency in grapevines: potential physiological targets for biotechnological improvement.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXislart7o%3D&md5=734321971275d7c35abb2338872715c2CAS |
García-Tejero IF, Durán-Zuazo VH, Muriel-Fernández JL, Jiménez-Bocanegra JA (2011) Linking canopy temperature and trunk diameter fluctuations with other physiological water status tools for water stress management in citrus orchards. Functional Plant Biology 38, 106–117.
| Linking canopy temperature and trunk diameter fluctuations with other physiological water status tools for water stress management in citrus orchards.Crossref | GoogleScholarGoogle Scholar |
Girona J, Mata M, del Campo J, Arbones A, Bartra E, Marsal J (2006) The use of midday leaf water potential for scheduling deficit irrigation in vineyards. Irrigation Science 24, 115–127.
| The use of midday leaf water potential for scheduling deficit irrigation in vineyards.Crossref | GoogleScholarGoogle Scholar |
Grant OM, Chaves MM, Jones HG (2006) Optimizing thermal imaging as a technique for detecting stomatal closure induced by drought stress under greenhouse conditions. Physiologia Plantarum 127, 507–518.
| Optimizing thermal imaging as a technique for detecting stomatal closure induced by drought stress under greenhouse conditions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XosVKgs74%3D&md5=d8f71bb7f31ed2541cd26424a908903eCAS |
Grant OM, Tronina L, Jones HG, Chaves MM (2007) Exploring thermal imaging variables for the detection of stress responses in grapevine under different irrigation regimes. Journal of Experimental Botany 58, 815–825.
| Exploring thermal imaging variables for the detection of stress responses in grapevine under different irrigation regimes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXislCrt7w%3D&md5=faa889818319332784d65ce42a0c3c12CAS |
Grant OM, Davies MJ, James CM, Johnson AW, Leinonen I, Simpson DW (2012) Thermal imaging and carbon isotope composition indicate variation amongst strawberry (Fragaria × ananassa) cultivars in stomatal conductance and water use efficiency. Environmental and Experimental Botany 76, 7–15.
| Thermal imaging and carbon isotope composition indicate variation amongst strawberry (Fragaria × ananassa) cultivars in stomatal conductance and water use efficiency.Crossref | GoogleScholarGoogle Scholar |
Hirayama M, Wada Y, Nemoto H (2006) Estimation of drought tolerance based on leaf temperature in upland rice breeding. Breeding Science 56, 47–54.
| Estimation of drought tolerance based on leaf temperature in upland rice breeding.Crossref | GoogleScholarGoogle Scholar |
Idso SB (1982) Non-water-stressed baselines – a key to measuring and interpreting plant water stress. Agricultural Meteorology 27, 59–70.
| Non-water-stressed baselines – a key to measuring and interpreting plant water stress.Crossref | GoogleScholarGoogle Scholar |
James RA, von Caemmerer S, Condon AG, Zwart AB, Munns R (2008) Genetic variation in tolerance to the osmotic stress component of salinity stress in durum wheat. Functional Plant Biology 35, 111–123.
| Genetic variation in tolerance to the osmotic stress component of salinity stress in durum wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXjsVKktbY%3D&md5=08269106842e8cca31361091868e0a25CAS |
Jones HG (1999) Use of thermography for quantitative studies of spatial and temporal variation of stomatal conductance over leaf surfaces. Plant, Cell & Environment 22, 1043–1055.
| Use of thermography for quantitative studies of spatial and temporal variation of stomatal conductance over leaf surfaces.Crossref | GoogleScholarGoogle Scholar |
Jones HG (2004) Application of thermal imaging and infrared sensing in plant physiology and ecophysiology. Advances in Botanical Research 41, 107–163.
| Application of thermal imaging and infrared sensing in plant physiology and ecophysiology.Crossref | GoogleScholarGoogle Scholar |
Jones HG, Stoll M, Santos T, Sousa C, Chaves MM, Grant OM (2002) Use of infra-red thermography for monitoring stomatal closure in the field: application to grapevine. Journal of Experimental Botany 53, 2249–2260.
| Use of infra-red thermography for monitoring stomatal closure in the field: application to grapevine.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xpt1Kgtbo%3D&md5=88c8c7af6a5f2d39f94a87290ffacf5aCAS |
Jones HG, Serraj R, Loveys BR, Xiong L, Wheaton A, Price AH (2009) Thermal infrared imaging of crop canopies for the remote diagnosis and quantification of plant responses to water stress in the field. Functional Plant Biology 36, 978–989.
| Thermal infrared imaging of crop canopies for the remote diagnosis and quantification of plant responses to water stress in the field.Crossref | GoogleScholarGoogle Scholar |
Kaplan H (2007) ‘Practical applications of infrared thermal sensing and imaging equipment.’ 3rd edn. (SPIE Press: Washington)
Kashiwagi J, Krishnamurthy L, Upadhyaya HD, Gaur PM (2008) Rapid screening technique for canopy temperature status and its relevance to drought tolerance improvement in chickpea. Journal of SAT Agricultural Research 6, 1–4.
Leinonen I, Grant OM, Tagliavia CPP, Chaves MM, Jones HG (2006) Estimating stomatal conductance with thermal imagery. Plant, Cell & Environment 29, 1508–1518.
| Estimating stomatal conductance with thermal imagery.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD28vnsVelug%3D%3D&md5=56fbb48b88d744a2d1cc438d5d47e0c2CAS |
Lovisolo C, Perrone I, Carra A, Ferrandino A, Flexas J, Medrano H, Schubert A (2010) Drought-induced changes in development and function of grapevine (Vitis spp.) organs and in their hydraulic and non hydraulic interactions at the whole plant level: a physiological and molecular update. Functional Plant Biology 37, 98–116.
| Drought-induced changes in development and function of grapevine (Vitis spp.) organs and in their hydraulic and non hydraulic interactions at the whole plant level: a physiological and molecular update.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtlyhsrs%3D&md5=a73b474d000c41034adec04bb179809fCAS |
Maroco JP, Rodrigues ML, Lopes C, Chaves MM (2002) Limitations to leaf photosynthesis in field-grown grapevine under drought – metabolic and modelling approaches. Functional Plant Biology 29, 451–459.
| Limitations to leaf photosynthesis in field-grown grapevine under drought – metabolic and modelling approaches.Crossref | GoogleScholarGoogle Scholar |
Medrano H, Escalona JM, Cifre J, Bota J, Flexas J (2003) A ten-year study on the physiology of two Spanish grapevine varieties under field conditions: effects of water availability from leaf photosynthesis to grape yield and quality. Functional Plant Biology 30, 607–619.
| A ten-year study on the physiology of two Spanish grapevine varieties under field conditions: effects of water availability from leaf photosynthesis to grape yield and quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXmvFSkt70%3D&md5=b0b98c119582386dae164f530dd7241dCAS |
Merlot S, Mustilli AC, Genty B, North H, Lefebvre V, Sotta B, Vavasseur A, Giraudat J (2002) Use of infrared thermal imaging to isolate Arabidopsis mutants defective in stomatal regulation. The Plant Journal 30, 601–609.
| Use of infrared thermal imaging to isolate Arabidopsis mutants defective in stomatal regulation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XlsFyktLc%3D&md5=64772760710310f659ca6deda1f28d8aCAS |
Merlot S, Leonhardt N, Fenzi F, Valon C, Costa M, Piette L, Vavasseur A, Genty B, Boivin K, Müller A, Giraudat J, Leung J (2007) Constitutive activation of a plasma membrane H+-ATPase prevents abscisic acid-mediated stomatal closure. EMBO Journal 26, 3216–3226.
| Constitutive activation of a plasma membrane H+-ATPase prevents abscisic acid-mediated stomatal closure.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXnsFejtrY%3D&md5=23994904b0fc0d3ec36d408f9d4a78d0CAS |
Möller M, Alchanatis V, Cohen Y, Meron M, Tsipris J, Naor A, Ostrovsky V, Sprintsin M, Cohen S (2007) Use of thermal and visible imagery for estimating crop water status of irrigated grapevine. Journal of Experimental Botany 58, 827–838.
| Use of thermal and visible imagery for estimating crop water status of irrigated grapevine.Crossref | GoogleScholarGoogle Scholar |
Moutinho-Pereira JM, Correia CM, Gonçalves BM, Bacelar EA, Torres-Pereira JM (2004) Leaf gas exchange and water relations of grapevines grown in three different conditions. Photosynthetica 42, 81–86.
| Leaf gas exchange and water relations of grapevines grown in three different conditions.Crossref | GoogleScholarGoogle Scholar |
Nilsson HE (1995) Remote sensing and image analysis in plant pathology. Annual Review of Phytopathology 33, 489–528.
| Remote sensing and image analysis in plant pathology.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXosFWisbo%3D&md5=0be535f8502c7ab2f012348a90c32679CAS |
Raskin I, Ladyman JAR (1988) Isolation and characterization of a barley mutant with abscisic acid-insensitive stomata. Planta 173, 73–78.
| Isolation and characterization of a barley mutant with abscisic acid-insensitive stomata.Crossref | GoogleScholarGoogle Scholar |
Rogiers SY, Greer DH, Hutton RJ, Landsberg JJ (2009) Does night-time transpiration contribute to anisohydric behaviour in a Vitis vinifera cultivar? Journal of Experimental Botany 60, 3751–3763.
| Does night-time transpiration contribute to anisohydric behaviour in a Vitis vinifera cultivar?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtV2ks77F&md5=332ea7e4d74f07fbdd681af0e15c0fefCAS |
Schultz HR (2003) Differences in hydraulic archicteture account for near isohydric and anisohydric behaviour of two field-grown Vitis vinifera L. varieties during drought. Plant, Cell & Environment 26, 1393–1405.
| Differences in hydraulic archicteture account for near isohydric and anisohydric behaviour of two field-grown Vitis vinifera L. varieties during drought.Crossref | GoogleScholarGoogle Scholar |
Schultz HR, Stoll M (2010) Some critical issues in environmental physiology of grapevines: future challenges and current limitations. Australian Journal of Grape and Wine Research 16, 4–24.
| Some critical issues in environmental physiology of grapevines: future challenges and current limitations.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXislartrc%3D&md5=ae2b9a48ea95ba5218188404d030b2ceCAS |
Sirault XRR, James RA, Furbank RT (2009) A new screening method for osmotic component of salinity tolerance in cereals using infrared thermography. Functional Plant Biology 36, 970–977.
| A new screening method for osmotic component of salinity tolerance in cereals using infrared thermography.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtlOgs7rI&md5=8f6a66f7c6ac3ef945abbc5e21d0db7eCAS |
Zia S, Spohrer K, Merkt N, Wenyong D, He X, Muller J (2009) Non invasive water status detection in grapevine. International Journal of Agricultural and Biological Engineering 2, 46–54.
