Maize STARCH SYNTHESIS REGULATING PROTEIN1 positively regulates starch biosynthesis in rice endosperm
Long Chen A # , Ming Du A B # , Long Wang A # , Wei Yu A , Yirong Chen A , Beijiu Cheng A and Jiandong Wu
A *
+ Author Affiliations
- Author Affiliations
A National Engineering Laboratory of Crop Stress Resistance, College of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, China.
B Shanghai Zhongke Quanyin Molecular Breeding Technology, Shanghai 200030, China.
* Correspondence to: wujiandong@ahau.edu.cn
# These authors contributed equally to this paper
Handling Editor: Thomas Roberts
Functional Plant Biology 49(9) 773-783 https://doi.org/10.1071/FP21338
Submitted: 7 April 2021 Accepted: 9 April 2022 Published: 2 May 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Starch is a major component of the endosperm, directly determining grain yield and quality. Although the key enzymes of starch synthesis have been identified and characterised, the regulatory mechanisms remain unclear. In this study, we identified the novel maize STARCH SYNTHESIS REGULATING PROTEIN1 (ZmSSRP1), which encodes a typical carbohydrate-binding module 48 (CBM48) protein. Expression analysis revealed that ZmSSRP1 was highly expressed in the maize endosperm, while transient expression in maize leaf protoplasts showed localisation in the plastids, dependent on the N-terminal transit peptide. In addition, overexpression of ZmSSRP1 in rice resulted in a decrease in grain thickness and the 1000-grain weight, as well as affecting the starch content and structure of the rice endosperm. The physicochemical properties of starch in the rice endosperm were also altered compared with the wild-type seeds. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was subsequently performed to determine the expression of starch synthesis-related genes, revealing upregulation of mRNA expression of most genes in the transgenic compared with wild-type lines. Collectively, these findings suggest that ZmSSRP1 acts as a potential regulator of starch synthesis, providing new insight for molecular breeding of high-yielding high-quality maize.
Keywords: CBM48, endosperm, maize, plastids, rice, seeds size, starch, ZmSSRP1.
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