PLDα1 and GPA1 are involved in the stomatal closure induced by Oridonin in Arabidopsis thaliana
Yue Zhang A , Ruirui Liu A , Yaping Zhou A , Simin Wang A , Bianfeng Zhang A , Juantao Kong A , Sheng Zheng A and Ning Yang
A B
+ Author Affiliations
- Author Affiliations
A College of Life Sciences, Northwest Normal University, Lanzhou 730070, People’s Republic of China.
B Corresponding author. Email: xbsd-yn@163.com
Functional Plant Biology 48(10) 1005-1016 https://doi.org/10.1071/FP21156
Submitted: 17 May 2021 Accepted: 3 June 2021 Published: 25 June 2021
Abstract
Oridonin is an important diterpenoid, which plays an important role in plant growth and development. PLDα1 and GPA1 are involved in many biotic or abiotic stresses. In this study, using the seedlings of Arabidopsis thaliana L. wild type (WT), PLDα1 defective mutant (pldα1), GPA1 defective mutant (gpa1) and pldα1/gpa1 double mutant as materials, the effect of stomatal apertures responding to Oridonin and the functions of PLDα1 and GPA1 in this response were investigated. The results showed that 60 μmol·L–1 of Oridonin induced stomatal closure and significantly increased the relative expression levels of GPA1 and PLDα1. Oridonin increased H2O2 accumulation in guard cells by inhibiting the antioxidant enzymes. The increase of H2O2 caused the expression of OST1, which is a positive regulatory gene for stomatal closure. Both PLDα1 and GPA1 were involved in Oridonin-induced stomatal closure and PLDα1 acted downstream of GPA1. The results suggested that Oridonin caused stomatal closure by affecting GPA1 and promoting PLDα1 to produce PA, and further accumulating H2O2 to upregulate gene OST1.
Keywords: Oridonin, phospholipase Dα1, GPA1, hydrogen peroxide, stomatal closure, Arabidopsis thaliana L.
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