Register      Login
Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
Shopping Cart: ( empty )
You are here: Home > Journals > AN > EA04272
RESEARCH ARTICLE
Contents Vol 46(1)

Genotypic variation in metribuzin tolerance in narrow-leafed lupin (Lupinus angustifolius L.)

P. Si A C , M. W. Sweetingham A B , B. J. Buirchell A B , D. G. Bowran B and T. Piper B
+ Author Affiliations
- Author Affiliations

A Centre for Legumes in Mediterranean Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

B Western Australian Department of Agriculture, Locked Bag No. 4, Bentley Delivery Centre, WA 6983, Australia.

C Corresponding author. Email: pingsi@cyllene.uwa.edu.au

Australian Journal of Experimental Agriculture 46(1) 85-91 https://doi.org/10.1071/EA04272
Submitted: 21 December 2004  Accepted: 4 August 2005   Published: 9 February 2006

Abstract

Tolerance to metribuzin herbicide is an essential agronomic trait for narrow-leafed lupin (L. angustifolius L.) grown in Western Australia (WA), however, metribuzin causes up to 30% yield loss in cv. Tanjil. Tanjil is widely used as a parent in the WA lupin breeding programme to provide anthracnose resistance. Hence, identification of genotypes tolerant to metribuzin and incorporation of this tolerance into the disease-resistant cultivar is necessary for maintaining lupin production. This study identified tolerance to metribuzin among lupin cultivars and advanced breeding lines under both controlled temperature and natural winter conditions.

Differences in dose responses between cultivars revealed that cv. Gungurru was tolerant and cv. Tanjil susceptible to metribuzin. Gungurru seedlings survived metribuzin applications of up to 1600 g/ha, whereas Tanjil seedlings exhibited zero survival at 800 g/ha. The rate of herbicide application that caused a 50% growth reduction (GR50, excluding dead plants) for Gungurru was 2 times greater than that for Tanjil. The level of tolerance in Gungurru is adequate to protect plants against metribuzin damage in the field. Large and consistent differences in tolerance between genotypes were identified among cultivars and advanced breeding lines across controlled temperatures (20°C during the day and 12°C at night) and in natural winter conditions. One breeding line (95L208–13–13) showed marginally better tolerance than Gungurru. A number of advanced breeding lines were as susceptible to metribuzin as Tanjil, indicating that it is very important to select for metribuzin tolerance concurrently with disease resistance in the breeding programme. Of the 6 measures of tolerance used in this study, leaf score proved to be the simplest and most effective measure and could be used for the selection of individual tolerant plants in segregating populations. Tolerance was independent of early vigour, suggesting that it is possible to combine both early vigour and tolerance into a cultivar for better weed management. In conclusion, breeding for metribuzin tolerance in lupin is feasible, and the screening method tested here was simple and consistent, which would assist a breeding programme in making rapid progress towards herbicide-tolerant plants.

Additional keywords: dose response, early vigour, herbicide tolerance, leaf score.


Acknowledgments

The Grains Research and Development Cooperation (GRDC) provided financial support for this research. We are grateful to Mr Shrestha for technical support and to the Western Australian Herbicide Resistance Initiative for providing the herbicide application facility.


References


Al-khatib K, Libbey C, Kadir S, Boydston R (1997) Differential varietal response of green pea (Pisum sativum) to metribuzin. Weed Technology 11, 775–781. open url image1

Barrentine WL, Hartwig EE, Edwards CJ, Kilen TC (1982) Tolerance of 3 soybean (Glycine max) cultivars to metribuzin. Weed Science 30, 344–348. open url image1

Caldwell CD, O’Sullivan PA (1985) Differential tolerance of 2 barley cultivars to metribuzin. Canadian Journal of Plant Science 65, 415–421. open url image1

Cooper JA, Bowran DG (1993) Metribuzin as a broad-leaved post-emergence herbicide for narrow leaf lupins. In ‘Proceedings of 10th Australian weeds conference, Brisbane, September 1993’. pp. 251–255. (The Weed Society of Queensland Inc.: Brisbane)

Dhammu HS, Piper T, Nicholson D (2003) Herbicide tolerance of new lupins. In ‘2003 Lupin updates — Western Australia’. (Ed. A McLarty) pp. 5–7. (Western Australian Department of Agriculture: South Perth)

Dhammu HS, Piper T, Nicholson D (2004) Herbicide tolerance of lupin varieties. In ‘2004 Weeds updates — Western Australia’. (Ed. A Douglas) pp. 47–48. (Western Australian Department of Agriculture: South Perth)

Edwards CJ, Barrentine WL, Kilen TC (1976) Inheritance of sensitivity to metribuzin in soybeans. Crop Science 16, 119–120. open url image1

Falb LN, Smith AE (1984) Metribuzin metabolism in soybeans. Characterization of the intraspecific differential tolerance. Journal of Agricultural and Food Chemistry 32, 1425–1428.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Frear DS, Mansager ER, Swason HR, Tanaka FS (1983) Metribuzin metabolism in tomato: isolation and identification of N-glucoside conjugates. Pesticide Biochemistry and Physiology 19, 270–281.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Frear DS, Swason HR, Mansager ER (1985) Alternate pathways of metribuzin metabolism in soybean: formation of N-glucoside and hologlutathione conjugates. Pesticide Biochemistry and Physiology 23, 56–65.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Genstat 5 Committee (1993) ‘Genstat 5 release 3 reference manual.’ (Clarendon Press: Oxford, UK)

Hardcastle WS (1974) Differences in the tolerance of metribuzin by varieties of soybeans. Weed Research 14, 181–184. open url image1

Hartwig EE, Barrentine WL, Edwards CJ (1980) Registration of Tracy-M soybeans. Crop Science 20, 825. open url image1

Phatak SC, Stephenson GR (1973) Influence of light and temperature on metribuzin phytotoxicity to tomato. Canadian Journal of Plant Science 53, 843–847. open url image1

Schroeder J, Banks PA, Nicholes RL (1985) Soft red winter wheat (Triticum aestivum) cultivar response to metribuzin. Weed Science 34, 66–69. open url image1

Seefeldt SS, Jensen JE, Fuerst EP (1995) Log-logistic analysis of herbicide dose response relationships. Weed Technology 9, 218–225. open url image1

Si P, Thurling N (2001) A greater relative growth rate of Brassica rapa L. at low temperatures increases biomass at anthesis. Australian Journal of Agricultural Research 52, 645–652.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Smith AE, Wilkinson RE (1974) Differential absorption, translocation and metabolism of metribuzin [4-amino-6-tert-butyl-3(methylthio)-as-triazine-5(4H)one] by soybean cultivars. Physiologia Plantarum 32, 253–257. open url image1

Stephenson GR, McLeod JE, Phatak SC (1976) Differential tolerance of tomato cultivars to metribuzin. Weed Science 24, 161–165. open url image1

Walsh MJ, Powles SB, Beard BR, Parkin BT, Porter SA (2004) Multiple-herbicide resistance across 4 modes of action in wild radish (Raphanus raphanistrum). Weed Science 52, 8–13. open url image1

Whan BR, Carlton GP, Anderson WK (1991) Potential for increasing early vigour and total biomass in spring wheat. I. Identification of genetic improvements. Australian Journal of Agricultural Research 42, 347–361.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Yang H, Boersma JG, You M, Buirchell BJ, Sweetingham MW (2004) Development and implementation of a sequence-specific PCR marker linked to a gene conferring resistance to anthracnose disease in narrow-leafed lupin (Lupinus angustifolius L.). Molecular Breeding 14, 145–151.
| Crossref | GoogleScholarGoogle Scholar | open url image1