Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

A comparison of fatty acid profiles and lipolysis during ripening of dry-cured loins obtained from a native pig breed (ChatoMurciano) and from a modern crossbreed pig

Luis Tejada A , Eva Salazar A C , Adela Abellán A , Begoña Peinado B , Juana Mulero A and Jose M. Cayuela A
+ Author Affiliations
- Author Affiliations

A Departamento de Tecnología de la Alimentación y Nutrición, Universidad Católica San Antonio, Campus de los Jerónimos, 30107 Guadalupe, Murcia, Spain.

B Equipo de Mejora Genética Animal, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), 30150 La Alberca, Murcia, Spain.

C Corresponding author. Email: esalazar@ucam.edu

Animal Production Science 56(11) 1928-1935 https://doi.org/10.1071/AN15033
Submitted: 26 September 2014  Accepted: 13 May 2015   Published: 6 August 2015

Abstract

The fatty acid composition of neutral lipids (NL), polar lipids (PL) and free fatty acids (FFA) was analysed in dry-cured loins obtained from the native pig breed Chato Murciano (CM) and from a modern crossbreed pig genotype (CG) during the ripening stage (between the 30 and 60 days of processing). Fatty acid concentrations from neutral lipids, polar lipids and free fatty acid fractions were affected by breed. With respect to ripening time, lipolysis was more intense in the CG than in CM product, resulting in a decrease in the concentrations of fatty acids in the NL and PL fractions, accompanied by a corresponding increase in FFA. Results for lipid determination provided evidence that the concentrations of the different groups of fatty acids within the lipid fractions depend on the breed. In order of abundance, the groups of fatty acids in the neutral lipid fraction were monounsaturated fatty acids (MUFA) > saturated fatty acids (SFA) > polyunsaturated fatty acids (PUFA) (59%, 37% and 4% in CM; 58%, 35% and 6% in CG) at 60 days of processing. In the polar lipid fraction, the order was SFA > PUFA > MUFA (44%, 29% and 27% in CM; 42%, 38% and 20% in CG), and in free fatty acid fraction, the order was MUFA > PUFA > SFA (40%, 30%, and 30% in CM; 39%, 32%, and 29% in CG) at 60 days of processing.

Additional keywords: cured products, lipid fractions, native species, quality for food processing.


References

Andrés AI, Cava R, Mayoral AI, Tejeda JF, Morcuende D, Ruiz J (2001) Oxidative stability and fatty acid composition of pig muscles as affected by rearing system, crossbreeding and metabolic type of muscle fibre. Meat Science 59, 39–47.
Oxidative stability and fatty acid composition of pig muscles as affected by rearing system, crossbreeding and metabolic type of muscle fibre.Crossref | GoogleScholarGoogle Scholar | 22062504PubMed |

Andrés AI, Cava R, Martin D, Ventanas J, Ruíz J (2005) Lipolysis in dry-cured ham: influence of salt content and processing conditions. Food Chemistry 90, 523–533.
Lipolysis in dry-cured ham: influence of salt content and processing conditions.Crossref | GoogleScholarGoogle Scholar |

Balsinde J, Winstead MV, Dennis EA (2002) Phospholipase A2 regulation of arachidonic acid mobilization. FEBS Letters 531, 2–6.
Phospholipase A2 regulation of arachidonic acid mobilization.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XotVOjt7k%3D&md5=7a56f31e0b89edb97a9d55afebc534c3CAS | 12401193PubMed |

Buscailhon S, Monin G (1994) Time-related changes in intramuscular lipids of lean tissue during processing of French dry-cured ham. Meat Science 37, 245–255.
Time-related changes in intramuscular lipids of lean tissue during processing of French dry-cured ham.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXkvVWgtrg%3D&md5=853c356a845d9c34c2ddcb8fec09bb6bCAS | 22059498PubMed |

Cava R, Ferrer JM, Estévez M, Morcuende D, Toldrá F (2004) Composition and proteolytic and lipolytic enzyme activities in muscle longissimus dorsi from Iberian pigs and industrial genotype pigs. Food Chemistry 88, 25–33.
Composition and proteolytic and lipolytic enzyme activities in muscle longissimus dorsi from Iberian pigs and industrial genotype pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXltFOmt70%3D&md5=c80b44781b829d4d662b19e53b4abc0bCAS |

Chiofalo B, Lo Presti V, Piccolo D, Arena G (2007) Nero Siciliano pig: effect of the diet on meat quality. Italian Journal of Animal Science 6, 679

Coutron-Gambotti C, Gandemer G, Casabianca F (1998) Effects of substituting a concentrated diet for chestnuts on the lipid traits of muscle and adipose tissues in corsican and Corsican × Large White pigs reared in a sylvo-pastoral system in Corsica. Meat Science 50, 163–174.
Effects of substituting a concentrated diet for chestnuts on the lipid traits of muscle and adipose tissues in corsican and Corsican × Large White pigs reared in a sylvo-pastoral system in Corsica.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXnsFCgtr0%3D&md5=3c473b300f60c943be255eee0666f50eCAS | 22060951PubMed |

Elias M, Sanabria C, Tirapicos Nunes J (2000) Effet du génotype du régime alimentaire sur quelques caractéristiques physique et chimiques du ‘Palaio’ et du ‘Jambon de barrancos’. Option Mediterranéennes 41, 217–225.

Folch J, Lees M, Stanley G (1957) A simple method for the isolation of total lipides from animal tissues. The Journal of Biological Chemistry 226, 495–509.

Franci O, Bozzi R, Pugliese C, Acciaioli A, Campodoni G, Gandini G (2005) Performance of Cinta Sense pigs and their crosses with Large White. I. Muscle and subcutaneus fat characteristics. Meat Science 69, 545–550.
Performance of Cinta Sense pigs and their crosses with Large White. I. Muscle and subcutaneus fat characteristics.Crossref | GoogleScholarGoogle Scholar | 22062994PubMed |

Gandemer G (2002) Lipids in muscle and adipose tissues, changes during processing and sensory properties of meat products. Meat Science 62, 309–321.
Lipids in muscle and adipose tissues, changes during processing and sensory properties of meat products.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XlsVOmtLw%3D&md5=d91b6fb07bb5347e1d30fd9c53a48d50CAS | 22061607PubMed |

Geri G, Franci O, Zappa A, Campodoni G (1984) Accrescimento relativo e distribuizone del grasso in suini maschi castrati e femmine da 20 a 200 chilogrami di peso vivo. Zootecnia e Nutrizione Animale 10, 365–380.

Girard JP, Denoyer C, Desmoulin B, Gandemer G (1983) Facteurs de variation de la composition en acides gras des tissus adipeux (bardiére) et musculaires de porc (long dorsal). Revue FranÇaise Corp Gras 30, 73–79.

Gray JI, Pearson AM (1987). Rancidity and xarmed-over flavour. Restructured meat and meat products. In ‘Advances in meat research 3’. (Eds AM Pearson, TR Dutson) pp. 221–269. (Van Nostrand Reinhold Company: New York)

Hernández P, Navarro JL, Toldrá F (1999) Lipolytic and oxidative changes in two Spanish pork loin products: dry-cured loin and pickled-cured loin. Meat Science 51, 123–128.
Lipolytic and oxidative changes in two Spanish pork loin products: dry-cured loin and pickled-cured loin.Crossref | GoogleScholarGoogle Scholar | 22061695PubMed |

Hu FB, Manson JE, Willet WC (2001) Types of dietary fats and risk of coronary heart disease: a critical review. Journal of the American College of Nutrition 30, 785–807.

Igene JO, Pearson AM, Gray JI (1981) Effect of length of frozen storage cooking and holding temperatures upon components phospholipids and the fatty acid composition of meat triglycerides and phospholipids. Food Chemistry 7, 289–303.
Effect of length of frozen storage cooking and holding temperatures upon components phospholipids and the fatty acid composition of meat triglycerides and phospholipids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL38XmslCiug%3D%3D&md5=aa7f4944bc67b8688dbac0f836db5d38CAS |

Jurado A, Carrapiso AI, Ventanas J, García C (2009) Changes in SPME extracted volatile compounds from Iberian ham during ripening. Grasas y Aceites 60, 262–270.
Changes in SPME extracted volatile compounds from Iberian ham during ripening.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXmtV2lu7s%3D&md5=5dc800f64a0e16d726fd36117cbad58bCAS |

Labroue F, Goumy S, Gruand J, Mourot J, Neelz V, Legault C (2000) Comparaison au Large White de quatre races locales porcines françaises pour les performances de croissance, de carcasse et de qualité de la viande. Journées De La Recherche Porcine En France 32, 403–411.

Lebecque A, Giraud G, Amblard C, Lucan A, Poma JP (2007) Analyse des profiles sensoriels de jambons secs fran¸cais et espagnols. Option Mediterranéennes 76, 333–337.

López-Bote C, Carmona Castaño JM, Solares M (2001). La calidad de la carne. In ‘Enciclopedia de la carne y los productos cárnicos’. (Ed. S Martín Bejarano) pp. 539–568. (Edicones Martín y Macías: Plasencia, Cáceres, Spain)

Madonia G, Diaferia C, Moretti VM, Margiotta S, Manganelli E, Pruiti V, Caprino F, D’Amico A (2007) Nero Siciliano pigs proposed as a traditional quality product: comparison between salami made from black pig’s meat and white pig’s meat. Option Mediterranéennes 76, 251–257.

Martín L, Córdoba JJ, Ventanas J, Antequera T (1999) Changes in intramuscular lipids during ripening of Iberian dry-cured ham. Meat Science 51, 129–134.
Changes in intramuscular lipids during ripening of Iberian dry-cured ham.Crossref | GoogleScholarGoogle Scholar | 22061696PubMed |

Monin G, Hortos M, Diaz I, Rock E, García-Regueiro JA (2003) Lipolysis and lipid oxidation during chilled storage of meat from Large White and Pietrain pigs. Meat Science 64, 7–12.
Lipolysis and lipid oxidation during chilled storage of meat from Large White and Pietrain pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xps12qsLo%3D&md5=a26b4e42367ca24df8dc0ca95264c908CAS | 22062656PubMed |

Motilva MJ, Toldrá F, Nieto P, Flores J (1993) Muscle lipolysis phenomena in the processing of dry-cured ham. Food Chemistry 48, 121–125.
Muscle lipolysis phenomena in the processing of dry-cured ham.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXmt1CltLw%3D&md5=5396cba59fd83de3456b7d853c133ff4CAS |

Muriel E, Antequera T, Petron J, Andres AI, Ruiz J (2005) Stereospecific analysis of fresh and dry-cured muscle phospholipids from Iberian pigs. Food Chemistry 90, 437–443.
Stereospecific analysis of fresh and dry-cured muscle phospholipids from Iberian pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXosFSksbo%3D&md5=be3dc35fd35cc0f4e8734d1f1b4b10e1CAS |

Muriel E, Andres AI, Petron MJ, Antequera T, Ruíz J (2007) Lipolytic and oxidative changes in Iberian dry-cured loin. Meat Science 75, 315–323.
Lipolytic and oxidative changes in Iberian dry-cured loin.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtFerurfO&md5=3ed5470af443a45a06e0d1791ab1ab56CAS | 22063664PubMed |

Peinado B, Poto A, Gil F, López G (2004) Characteristics of the carcass and meat of the Chato Murciano pig. Livestock Science 90, 285–292.
Characteristics of the carcass and meat of the Chato Murciano pig.Crossref | GoogleScholarGoogle Scholar |

Peinado B, Almela L, Duchi N, Poto A (2009) Parámetros de calidad en la canal y en la carne de cerdo Chato Murciano. Eurocarne 173, 64–80.

Perna A, Simonetti A, Intaglietta I, Gambacorta E (2014) Quality and sensory characteristics of Culatello dry cured product obtained from the Italian autochthonous pig Suino Nero Lucano and from a modern crossbred pig. Animal Production Science
Quality and sensory characteristics of Culatello dry cured product obtained from the Italian autochthonous pig Suino Nero Lucano and from a modern crossbred pig.Crossref | GoogleScholarGoogle Scholar |

Perna A, Simonetti A, Intaglietta I, Gambacorta E (2015) Fatty acids composition, cholesterol and vitamin E contents of longissimus dorsi and semitendinosus muscles of Suino Nero Lucano pigs slaughtered at two different weights. Animal Production Science 55, 1037–1043.
Fatty acids composition, cholesterol and vitamin E contents of longissimus dorsi and semitendinosus muscles of Suino Nero Lucano pigs slaughtered at two different weights.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhtVOktrvE&md5=c9402795308774848790b7b0b063b47cCAS |

Poto A, Lobera JB, Peinado B (2000) Razas autóctonas de Murcia. Estimación del censo y aptitudes. Archivos de zootecnia 49, 107–114. (In Spanish).

Poto A, Galián M, Peinado B (2007) Chato Murciano pig and its crosses with Iberian and Large White pigs, reared outdoors. Comparative study of the carcass and meat characteristics. Livestock Science 111, 96–103.
Chato Murciano pig and its crosses with Iberian and Large White pigs, reared outdoors. Comparative study of the carcass and meat characteristics.Crossref | GoogleScholarGoogle Scholar |

Pugliese C, Sirtori F (2012) Quality of meat and meat products produced from southern European pig breeds. Meat Science 90, 511–518.
Quality of meat and meat products produced from southern European pig breeds.Crossref | GoogleScholarGoogle Scholar | 22030111PubMed |

Pugliese C, Sirtori F, Ruiz J, Martin D, Parenti S, Franci O (2009) Effect of pasture on chestnut or acorn on fatty acid composition and aromatic profile of fat of cinta senese dry-cured ham. Grasas y Aceites 60, 271–276.
Effect of pasture on chestnut or acorn on fatty acid composition and aromatic profile of fat of cinta senese dry-cured ham.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXmtV2lu7g%3D&md5=9f5175554f1ed866bb9375cfec347239CAS |

Ramírez R, Cava R (2007) Effect of Iberian × duroc genotype on dry-cured loin quality. Meat Science 76, 333–341.
Effect of Iberian × duroc genotype on dry-cured loin quality.Crossref | GoogleScholarGoogle Scholar |

Rosell CM, Toldrá F (1998) Comparison of muscle proteolytic and lypolitic enzime levels in raw hams from Iberian and White pigs. Journal of the Science of Food and Agriculture 76, 117–122.
Comparison of muscle proteolytic and lypolitic enzime levels in raw hams from Iberian and White pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXht1aksLk%3D&md5=21cb313c61efcf6e3799bed14f0f8959CAS |

Ruíz J, Antequera T, Andres AI, Petron MJ, Muriel E (2004) Improvement of a solid phase extraction method for anlysis of lipid fractions in muscle foods. Analytica Chimica Acta 520, 201–205.
Improvement of a solid phase extraction method for anlysis of lipid fractions in muscle foods.Crossref | GoogleScholarGoogle Scholar |

Salazar E, Cayuela JM, Abellán A, Poto A, Peinado B, Tejada L (2013) A comparison of the quality of dry-cured loins obtained from the native pig breed (Chato Murciano) and from a modern crossbreed pig. Animal Production Science 53, 352–359.
A comparison of the quality of dry-cured loins obtained from the native pig breed (Chato Murciano) and from a modern crossbreed pig.Crossref | GoogleScholarGoogle Scholar |

Salazar E, Abellán A, Cayuela JM, Poto A, Girón F, Zafrilla P, Tejada L (2015) Effect of processing time on the quality of dry-cured ham obtained from a native pig breed (Chato Murciano). Animal Production Science 55, 113–121.
Effect of processing time on the quality of dry-cured ham obtained from a native pig breed (Chato Murciano).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXitVKqsrrJ&md5=820559139c8d43ca8e6dbb8fe7cc51b7CAS |

Salvatori G, Filetti F, Di Cesare C, Maiorano G, Oriani G (2008) Lipid composition of meat and backfat from casertana purebred and crossbred pig reared outdoors. Meat Science 80, 623–631.
Lipid composition of meat and backfat from casertana purebred and crossbred pig reared outdoors.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtVKmtbvI&md5=5b593fc9be99f37d83013f3b7d59102aCAS | 22063574PubMed |

Sandler SR, Karo W (1992) ‘Source book of advances organic laboratory preparations.’ (Academic Press:.San Diego, CA)

Sirtori F, Pugliese C, Parenti S, D’Adorante S, Franci O (2011) Fattening with chestnut in Cinta Senese pigs: effect on meat and fat characteristics. In ‘New trend for innovation in the Mediterranean production’. (Eds RBouche, ADerkimba, F Casabianca) pp. 117–122. (Wageningen Academic Publishers: Wageningen, The Netherlands)

Tejeda JF, Gndemer G, Antequera T, Viau M, García C (2002) Lipid traits of muscles as related to genotype and fattening diet in Iberian pigs: total intramuscular lipids and triacylglycerols. Meat Science 60, 357–363.
Lipid traits of muscles as related to genotype and fattening diet in Iberian pigs: total intramuscular lipids and triacylglycerols.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XhsFyh&md5=a9c74c1576b8b07ad063d041ada63eebCAS | 22063638PubMed |

Ventanas S, Ventanas J, Jurado A, Estévez M (2006) Quality traits in muscle biceps femoris and back-fat from purebred Iberian and reciprocal Iberian × duroc crossbred pigs. Meat Science 73, 651–659.
Quality traits in muscle biceps femoris and back-fat from purebred Iberian and reciprocal Iberian × duroc crossbred pigs.Crossref | GoogleScholarGoogle Scholar | 22062566PubMed |

Ventanas S, Ventanas J, Tovar J, García C, Estévez M (2007) Extensive feeding versus oleic acid and tocopherol enriched mixed diets for the production of Iberian dry-cured hams: effect on chemical composition, oxidative status and sensory traits. Meat Science 77, 246–256.
Extensive feeding versus oleic acid and tocopherol enriched mixed diets for the production of Iberian dry-cured hams: effect on chemical composition, oxidative status and sensory traits.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXnsFKqtr0%3D&md5=326a747eb2d2f9ba823229fecc6d44fbCAS | 22061597PubMed |

Wood JD, Lister D (1973) The fatty acid phospholipid composition of longissimus dorsi muscle from Pietrain and Large White pigs. Journal of the Science of Food and Agriculture 24, 1449–1456.
The fatty acid phospholipid composition of longissimus dorsi muscle from Pietrain and Large White pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2cXjsF2nsg%3D%3D&md5=84d718b56484b38f47e60a19c658ff7eCAS | 4763683PubMed |

Wood JD, Nute GR, Richardson RI, Whittington FM, Southwood O, Plastow G, Mansbridge JR, da Costa N, Chang KC (2004) Effects of breed diet and muscle on fat deposition and eating quality in pigs. Meat Science 67, 651–667.
Effects of breed diet and muscle on fat deposition and eating quality in pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXisFyrtbo%3D&md5=0a4538fa7a270485e15cdafb745e6c54CAS | 22061815PubMed |

Zullo A, Barone CMA, Colatruglio P, Diaferia C, Genovino G, Matassino D (2007) Sensory evaluation of dry cured coppa in some swine autochthonous genetic type. Option Mediterranéennes 76, 295–298.