Single-cell RNA-sequencing analysis and characterisation of testicular cells in giant panda (Ailuropoda melanoleuca)
Yi Zheng
A , Yuliang Liu B C , Rong Hou B C , Keyu Shi B , Yijiao Chen B , Tongying Feng B and Junhui An B C *
+ Author Affiliations
- Author Affiliations
A Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
B Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan 610081, China.
C Sichuan Academy of Giant Panda, Chengdu, Sichuan 610000, China.
Reproduction, Fertility and Development 34(14) 933-943 https://doi.org/10.1071/RD22039
Published online: 12 August 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Context: The giant panda (Ailuropoda melanoleuca) is a rare and endangered species to be preserved in China. The giant panda has a low reproductive capacity, and due to the scarcity of samples, studies on testes from giant panda are very limited, with little knowledge about the process of spermatogenesis in this species.
Aims: To establish the gene expression profiles in cells from the testis of a giant panda.
Methods: The 10× Genomics single-cell RNA-sequencing platform was applied to cells from the testis of an adult giant panda.
Key results: We identified eight testicular cell types including six somatic and two germ cell types from our single-cell RNA-sequencing datasets. We also identified the differentially expressed genes (DEGs) in each cell type, and performed functional enrichment analysis for the identified testicular cell types. Furthermore, by immunohistochemistry we explored the protein localisation patterns of several marker genes in testes from giant panda.
Conclusions: Our study has for the first time established the gene expression profiles in cells from the testis of a giant panda.
Implications: Our data provide a reference catalogue for spermatogenesis and testicular cells in the giant panda, laying the foundation for future breeding and preservation of this endangered species.
Keywords: giant panda, molecular marker, scRNA-seq, spermatid, spermatocyte, spermatogenesis, spermatogonia, testis.
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