Twenty microsatellite loci for population and conservation genetic studies of the wedge-tailed eagle (Aquila audax)
J. J. Austin A B G , L. Olivier A , D. Nankervis C , W. E. Brown D , M. G. Gardner E F and C. P. Burridge CA Australian Centre for Ancient DNA, School of Earth and Environmental Sciences and Environment Institute, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia.
B Sciences Department, Museum Victoria, Carlton Gardens, Melbourne, Vic. 3001, Australia.
C School of Zoology, University of Tasmania, Private Bag 5, Hobart, Tas. 7001, Australia.
D Department of Primary Industry, Parks, Water and Environment, Hobart, Tas. 7001, Australia.
E School of Biological Sciences, Flinders University, Adelaide, SA 5042, Australia.
F Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA 5001, Australia.
G Corresponding author. Email: jeremy.austin@adelaide.edu.au
Australian Journal of Zoology 62(3) 235-237 https://doi.org/10.1071/ZO14030
Submitted: 7 May 2014 Accepted: 9 June 2014 Published: 30 June 2014
Abstract
Twenty di- to pentanucleotide microsatellites are reported for the wedge-tailed eagle (Aquila audax), a large raptor from Australia, Tasmania, and New Guinea. These loci were tested for variation among 49 individuals. All loci are polymorphic with 2–14 alleles per locus, and observed heterozygosities ranged between 0.021 and 0.898. Genotype frequencies for all loci did not differ significantly from Hardy–Weinberg equilibrium and there was no evidence of linkage disequilibrium. These markers will be used to assess population structure and conservation genetics of this species, focusing on population differentiation and gene flow between Tasmanian and mainland populations and conservation genetics of the endangered Tasmanian population.
Additional keyword: raptor.
References
Brownstein, M. J., Carpten, D, and Smith, J. R. (1996). Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. BioTechniques 20, 1004–1010.| 1:CAS:528:DyaK28XjtlGmtr8%3D&md5=c81217e81051389efc81f04eb510fce1CAS | 8780871PubMed |
Burridge, C. P., Brown, W. E., Wadley, J., Nankervis, D. L., Olivier, L., Gardner, M. G., Hull, C., Barbour, R., and Austin, J. J. (2013). Did postglacial sea-level changes initiate the evolutionary divergence of a Tasmanian endemic raptor from its mainland relative? Proceedings of the Royal Society B: Biological Sciences 280, 20132448.
| Did postglacial sea-level changes initiate the evolutionary divergence of a Tasmanian endemic raptor from its mainland relative?Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC2c7htlehtw%3D%3D&md5=f03ab47702153140c81c741f00b27577CAS | 24174114PubMed |
Gardner, M. G., Fitch, A. J., Bertozzi, T., and Lowe, A. J. (2011). Rise of the machines – recommendations for ecologists when using next generation sequencing for microsatellite development. Molecular Ecology Resources 11, 1093–1101.
| Rise of the machines – recommendations for ecologists when using next generation sequencing for microsatellite development.Crossref | GoogleScholarGoogle Scholar | 21679314PubMed |
Meglécz, E., Costedoat, C., Dubut, V., Giles, A., Malausa, T., Pech, N., and Martin, J. F. (2010). QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26, 403–404.
| QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects.Crossref | GoogleScholarGoogle Scholar | 20007741PubMed |
Meglécz, E., Nève, G., Biffin, E., and Gardner, M. G. (2012). Breakdown of phylogenetic signal: a survey of microsatellite densities in 454 shotgun sequences from 154 non model eukaryote species. PLoS ONE 7, e40861.
| Breakdown of phylogenetic signal: a survey of microsatellite densities in 454 shotgun sequences from 154 non model eukaryote species.Crossref | GoogleScholarGoogle Scholar | 22815847PubMed |
Raymond, M., and Rousset, F. (1995). GENEPOP (Version-1.2) – Population-genetics software for exact tests and ecumenicism. The Journal of Heredity 86, 248–249.
Rice, T. K., Schork, N. J., Rao, D. C., Rao, D. C., and Gu, C. C. (2008). Methods for handling multiple testing. In ‘Advances in Genetics’. (Eds D. C. Rao and C. C. Gu.) pp. 293–308. (Academic Press: New York.)
Rousset, F. (2008). GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Molecular Ecology Resources 8, 103–106.
| GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux.Crossref | GoogleScholarGoogle Scholar | 21585727PubMed |
Rousset, F., and Raymond, M. (1995). Testing heterozygote excess and deficiency. Genetics 140, 1413–1419.
| 1:STN:280:DyaK287itVemtA%3D%3D&md5=f66ad2532bbfd87092f84b3c35ae0bebCAS | 7498780PubMed |
Van Oosterhout, C., Hutchinson, W. F., Wills, D. P. M., and Shipley, P. (2004). MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4, 535–538.
| MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXnvFOktb8%3D&md5=642d3c27a53cc46b25cea2a96ea87beeCAS |
