Development of a multiplex panel of microsatellite markers for two species of gliding marsupials, Petaurus breviceps and Petaurus norfolcensis
M. Malekian A B C G , R. Y. Dudaniec D , K. M. Saint E , S. M. Carthew B F and S. J. B. Cooper B C EA Department of Natural Resources, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
B School of Earth and Environmental Sciences, The University of Adelaide, Adelaide, SA 5005, Australia.
C Centre for Evolutionary Biology and Biodiversity, The University of Adelaide, Adelaide, SA 5005, Australia.
D Department of Biology, Lund University, Lund, SE-22362, Sweden.
E Evolutionary Biology Unit, South Australian Museum, Adelaide, SA 5000, Australia.
F Research Institute for Environment and Livelihoods, Charles Darwin University, Darwin, NT 0909, Australia.
G Corresponding author. Email: mmalekian@cc.iut.ac.ir
Australian Journal of Zoology 61(6) 475-478 https://doi.org/10.1071/ZO14002
Submitted: 28 January 2014 Accepted: 11 March 2014 Published: 3 April 2014
Abstract
Here, we describe the development of seven new microsatellite loci from Petaurus breviceps. Together with eight loci from previous studies of gliders, we tested their utility for amplification, multiplexing and polymorphism in two glider species, P. breviceps and P. norfolcensis. Of the 15 loci tested, all were polymorphic in P. breviceps and 12 were polymorphic in P. norfolcensis. Overall, 260 sugar gliders from 13 sites in south-eastern South Australia and 106 squirrel gliders collected throughout south-east Queensland were used in analyses. Numbers of alleles per locus ranged from 4 to 27 in P. breviceps and from 2 to 44 in P. norfolcensis. Observed heterozygosity ranged between 0.438 and 0.904 in P. breviceps and between 0.189 and 0.981 in P. norfolcensis. Within the populations analysed, one of the 15 loci for P. breviceps and two of the 12 loci for P. norfolcensis deviated from Hardy–Weinberg equilibrium. The microsatellite loci will provide valuable tools for further study of social organisation, mating systems and population biology of these gliding marsupials.
Additional keywords: 454 sequencing, habitat fragmentation, microsatellites, population structure.
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