Genetic differentiation of regional populations of the widespread Asiatic toad (Bufo gargarizans), as revealed by development of novel microsatellite markers
T. Pan A C , P. Yan A C , M. Yang A , H. Wang A , I. Ali A , M. Ayub A , J. H. Zhang A , J. J. Wang A , E. Li A , H. Xue A , B. W. Zhang B D and X. B. Wu
A D
+ Author Affiliations
- Author Affiliations
A Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 241000, Anhui, China.
B School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
C These authors contributed equally to this work.
D Corresponding authors. Email: zhangbw@ahu.edu.cn, wuxb@ahnu.edu.cn
Australian Journal of Zoology 66(6) 335-342 https://doi.org/10.1071/ZO18059
Submitted: 20 August 2018 Accepted: 3 June 2019 Published: 11 July 2019
Abstract
Dispersal is a key component of a species’ life history, by influencing population persistence, genetic structure, adaptation and maintenance of genetic diversity. The Asiatic toad (Bufo gargarizans) is a widespread species in east Asia. However, we still have no knowledge of what kind of geographical scale equates to genetic differentiation within B. gargarizans. In this study, the population genetics of B. gargarizans was studied at five localities, with the Yangtze River running through the sampling area, in order to detect the level of genetic differentiation and the natural barriers to the species’ dispersal on a small geographic scale, by means of the development and use of novel microsatellite loci. These markers revealed a relatively high level of genetic diversity. Distinct genetic structure among populations in B. gargarizans was observed, as described by genetic distance, AMOVA, PCA and Geneland results. A weak but significant positive correlation between genetic distance and geographical distance. The combination of these findings suggests that the Yangtze River and geographic distance may act as effective barriers for B. gargarizans. These results serve as benchmark data for understanding the impacts of dispersal barriers and continued landscape research on B. gargarizans.
Additional keywords: genetic structure, geographic distance, population genetics, Yangtze River.
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