Isolation and characterisation of 14 polymorphic microsatellite loci for the plant-louse Trioza barrettae (Hemiptera : Triozidae)
Melinda L. Moir A , Yvette Hitchen B , Michael G. Gardner C , David J. Coates D and W. Jason Kennington E FA School of Plant Biology, Ecosystem Restoration and Intervention Ecology Research Group, The University of Western Australia, Crawley, WA 6009, Australia.
B Helix Molecular Solutions, PO Box 155, Leederville, WA 6903, Australia.
C School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia; Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide, SA 5005, Australia; and Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA 5000, Australia.
D Western Australian Department of Parks and Wildlife, Locked Bag 104, Bentley Delivery Centre, WA 6983, Australia.
E Centre for Evolutionary Biology, School of Animal Biology, The University of Western Australia, Crawley, WA 6009, Australia.
F Corresponding author. Email: jason.kennington@uwa.edu.au
Australian Journal of Zoology 63(3) 201-203 https://doi.org/10.1071/ZO15009
Submitted: 25 February 2015 Accepted: 12 July 2015 Published: 30 July 2015
Abstract
We describe the isolation and development of 14 polymorphic microsatellite loci for the critically endangered plant-louse Trioza barrettae (Hemiptera : Triozidae). The loci were tested in 25 individuals from a single population situated in the south-west of Western Australia. No evidence of linkage disequilibrium was detected between any pair of loci. However, eight loci showed significant departures from Hardy–Weinberg expectations. The number of alleles per locus ranged from 2 to 27.
Additional keywords: lerp insects, microsatellites, Psylloidea.
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