Isolation and characterisation of polymorphic microsatellite loci for the neriid fly Telostylinus angusticollis using MiSeq sequencing
Anna M. Kopps A B , Russell Bonduriansky A , Anthony S. Gilchrist A and Angela J. Crean A
+ Author Affiliations
- Author Affiliations
A Evolution and Ecology Research Centre and School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
B Corresponding author. Email: anna.kopps@gmx.com
Australian Journal of Zoology 60(6) 388-391 https://doi.org/10.1071/ZO13017
Submitted: 11 February 2013 Accepted: 22 April 2013 Published: 16 May 2013
Abstract
The neriid fly Telostylinus angusticollis is being developed as a model organism for experimental research on developmental plasticity, nongenetic inheritance, ageing and sexual selection. No genetic information is currently available for T. angusticollis, so to facilitate paternity analyses for experimentation we characterised 17 polymorphic microsatellites for this species based on MiSeq sequences. The loci had, on average, 4.1 alleles and the observed heterozygosity ranged from 0.250 to 0.889. We show that MiSeq can be used successfully to develop microsatellite markers.
Additional keywords: Neriidae, paternal effect.
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