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Australian Journal of Zoology Australian Journal of Zoology Society
Evolutionary, molecular and comparative zoology
RESEARCH ARTICLE

Identification of the rainbowfish in Lake Eacham using DNA sequencing

Culum Brown A B , Yagiz Aksoy A , Hilal Varinli A and Michael Gillings A
+ Author Affiliations
- Author Affiliations

A Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia.

B Corresponding author. Email: Culum.Brown@mq.edu.au

Australian Journal of Zoology 60(5) 334-339 https://doi.org/10.1071/ZO12106
Submitted: 16 October 2012  Accepted: 4 February 2013   Published: 20 February 2013

Abstract

The Lake Eacham rainbowfish (Melanotaenia eachamensis) was once thought to be confined to its type locality within the Lake Eacham World Heritage National Park. M. eachamensis disappeared from the lake following the translocation of several species into the lake and the species was pronounced extinct in the wild in 1987. In a 2007 survey we noticed that rainbowfish were present in the lake once again. We used a molecular marker to identify these fish and the likely source population. Analysis of the D-loop region of mitochondrial DNA revealed that the species now present in the lake is Melanotaenia splendida, and is most closely related to several M. splendida populations in the immediate vicinity. Here we explore a range of scenarios that may have led to this colonisation event and highlight the dangers associated with translocation.

Additional keywords: colonisation, Melanoteania, translocation.


References

Allen, G. R. (1989). Lake Eacham rainbowfish rediscovered? Fishes of Sahul 5, 217–219.

Allen, G. R., and Cross, N. J. (1982). ‘Rainbowfishes of Australia and Papua New Guinea.’ (Angus and Robertson: Sydney.)

Barlow, C. G., Hogan, A. E., and Rogers, L. J. (1987). Implication of translocated fishes in the apparent extinction in the wild of the Lake Eacham rainbowfish, Melanotaenia eachamensis. Australian Journal of Marine and Freshwater Research 38, 897–902.
Implication of translocated fishes in the apparent extinction in the wild of the Lake Eacham rainbowfish, Melanotaenia eachamensis.Crossref | GoogleScholarGoogle Scholar |

Brown, C. (2003). Habitat–predator association and avoidance in rainbowfish (Melanotaenia spp.). Ecology Freshwater Fish 12, 118–126.
Habitat–predator association and avoidance in rainbowfish (Melanotaenia spp.).Crossref | GoogleScholarGoogle Scholar |

Brown, C., and Day, R. (2002). The future of stock enhancements: bridging the gap between hatchery practice and conservation biology. Fish and Fisheries 3, 79–94.
The future of stock enhancements: bridging the gap between hatchery practice and conservation biology.Crossref | GoogleScholarGoogle Scholar |

Brown, C., and Laland, K. N. (2001). Social learning and life skills training for hatchery reared fish. Journal of Fish Biology 59, 471–493.
Social learning and life skills training for hatchery reared fish.Crossref | GoogleScholarGoogle Scholar |

Brown, C., and Warburton, K. (1997). Predator recognition and anti-predator responses in the rainbowfish Melanotaenia eachamensis. Behavioral Ecology and Sociobiology 41, 61–68.
Predator recognition and anti-predator responses in the rainbowfish Melanotaenia eachamensis.Crossref | GoogleScholarGoogle Scholar |

Brown, C., and Warburton, K. (1999). Differences in timidity and escape responses between predator-naïve and predator-sympatric rainbowfish populations. Ethology 105, 491–502.
Differences in timidity and escape responses between predator-naïve and predator-sympatric rainbowfish populations.Crossref | GoogleScholarGoogle Scholar |

Crowley, E. L. M., and Ivantsoff, W. (1991). Genetic similarity among populations of rainbowfishes (Pices: Melanotaeniidae) from Atherton Tableland, northern Queensland. Ichthyological Exploration of Freshwaters 2, 129–137.

Faulks, L., Gilligan, D., and Beheregaray, L. (2010). Evolution and maintenance of divergent lineages in an endangered freshwater fish, Macquaria australasica. Conservation Genetics 11, 921–934.
Evolution and maintenance of divergent lineages in an endangered freshwater fish, Macquaria australasica.Crossref | GoogleScholarGoogle Scholar |

Kydd, E., and Brown, C. (2009). Loss of shoaling preference for familiar individuals in captive-reared crimson spotted rainbowfish Melanotaenia duboulayi. Journal of Fish Biology 74, 2187–2195.
Loss of shoaling preference for familiar individuals in captive-reared crimson spotted rainbowfish Melanotaenia duboulayi.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cjnt1Olsg%3D%3D&md5=b724fc618e98ba47f6f935f5383b1ca7CAS |

Lee, W.-J., Conroy, J., Howell, W. H., and Kocher, T. D. (1995). Structure and evolution of teleost mitochondrial control regions. Journal of Molecular Evolution 41, 54–66.
Structure and evolution of teleost mitochondrial control regions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXmt1yktrw%3D&md5=59b3721f3f456d7662e14badec10e4a0CAS |

Lintermans, M. (2004). Human-assisted dispersal of alien freshwater fish in Australia. New Zealand Journal of Marine and Freshwater Research 38, 481–501.
Human-assisted dispersal of alien freshwater fish in Australia.Crossref | GoogleScholarGoogle Scholar |

McGuigan, K., Zhu, D., Allen, G. R., and Moritz, C. (2000). Phylogenetic relationships and historical biogeography of melanotaeniid fishes in Australia and New Guinea. Marine and Freshwater Research 51, 713–723.
Phylogenetic relationships and historical biogeography of melanotaeniid fishes in Australia and New Guinea.Crossref | GoogleScholarGoogle Scholar |

McGuigan, K., Franklin, C. E., Moritz, C., and Blows, M. W. (2003). Adaptation of rainbow fish to lake and stream habitats. Evolution 57, 104–118.

Page, T. J., Sharma, S., and Hughes, J. M. (2004). Deep phylogenetic structure has conservation implications for ornate rainbowfish (Melanotaeniidae: Rhadinocentrus ornatus) in Queensland, eastern Australia. Marine and Freshwater Research 55, 165–172.
Deep phylogenetic structure has conservation implications for ornate rainbowfish (Melanotaeniidae: Rhadinocentrus ornatus) in Queensland, eastern Australia.Crossref | GoogleScholarGoogle Scholar |

Pusey, B. J., Bird, J., Kennard, M. J., and Arthington, A. H. (1997). Distribution of the Lake Eacham rainbowfish in the Wet Tropics region, north Queensland. Australian Journal of Zoology 45, 75–84.
Distribution of the Lake Eacham rainbowfish in the Wet Tropics region, north Queensland.Crossref | GoogleScholarGoogle Scholar |

Pusey, B. J., Kennard, M. J., and Arthington, A. H. (2004). ‘Freshwater Fishes of North-eastern Australia.’ (CSIRO Publishing: Melbourne.)

Sunnucks, P., and Hales, D. F. (1996). Numerous transposed sequences of mitochondrial cytochrome oxidase I–II in aphids of the genus Sitobion (Hemiptera: Aphididae). Molecular Biology and Evolution 13, 510–524.
Numerous transposed sequences of mitochondrial cytochrome oxidase I–II in aphids of the genus Sitobion (Hemiptera: Aphididae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28Xht1Kgurk%3D&md5=96aa0f41c00a5a3f1b266f7f0e7a3afcCAS |

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 2731–2739.
MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXht1eiu73K&md5=8f36c83b0d68101124972bf90ddbf065CAS |

Thompson, J. D., and Higgins, D. G. Thompson, J. D., and Higgins, D. G. (1994). Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673–4680.
Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXitlSgu74%3D&md5=5f38ef69f1e1a885f614d1b0765b3264CAS |

Timms, B. V. (1979). The benthos of some lakes in northeastern Queensland. Proceedings of the Royal Society of Queensland 90, 57–64.

Unmack, P. J. (2001). Biogeography of Australian freshwater fishes. Journal of Biogeography 28, 1053–1089.
Biogeography of Australian freshwater fishes.Crossref | GoogleScholarGoogle Scholar |

Wager, R. (1993). The distribution and conservation status of Queensland freshwater fishes. Queensland Department of Primary Industries, Brisbane.

Zhu, D. Q., Jamieson, B. G. M., Hugall, A., and Moritz, C. (1994). Sequence evolution and phylogenetic signal in control-region and cytochrome-b sequences of rainbow fishes (Melanotaeniidae). Molecular Biology and Evolution 11, 672–683.
| 1:CAS:528:DyaK2cXkslGjsL0%3D&md5=8a2a0c680a062c781b1fca4551940202CAS |

Zhu, D. Q., Degnan, S., and Moritz, C. (1998). Evolutionary distinctiveness and status of the endangered Lake Eacham rainbowfish (Melanotaenia eachamensis). Conservation Biology 12, 80–93.
Evolutionary distinctiveness and status of the endangered Lake Eacham rainbowfish (Melanotaenia eachamensis).Crossref | GoogleScholarGoogle Scholar |

Zuberi, A., Ali, S., and Brown, C. (2011). A non-invasive assay for monitoring stress responses: A comparison between wild and captive-reared rainbowfish (Melanoteania duboulayi). Aquaculture 321, 267–272.
A non-invasive assay for monitoring stress responses: A comparison between wild and captive-reared rainbowfish (Melanoteania duboulayi).Crossref | GoogleScholarGoogle Scholar |