Isolation and characterisation via 454 sequencing of microsatellites from the tawny frogmouth, Podargus strigoides (Class Aves, Family Podargidae)
Fiona E. Hogan A E , Marian Weaving B , Gregory R. Johnston C and Michael G. Gardner DA School of Applied Sciences and Engineering, Monash University, Northways Road, Churchill, Vic. 3842, Australia.
B School of Life and Environmental Sciences, Deakin University, 221 Burwood Highway, Burwood, Vic. 3125, Australia.
C Vertebrates Section, South Australian Museum, North Terrace, Adelaide, SA 5000, Australia.
D School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia; Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide, SA 5005, Australia; and Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA 5000, Australia.
E Corresponding author. Email: Fiona.Hogan@monash.edu
Australian Journal of Zoology 60(2) 133-136 https://doi.org/10.1071/ZO12062
Submitted: 14 June 2012 Accepted: 2 August 2012 Published: 17 September 2012
Abstract
We isolated 24 novel polymorphic microsatellite markers from the tawny frogmouth, a nocturnal bird endemic to Australia, which has successfully adapted to urban environments. Initially, 454 shotgun sequencing was used to identify 733 loci with primers designed. Of these, we trialled 30 in the target species of which all amplified a product of expected size. Subsequently, all 30 of these loci were screened for variation in 25 individuals, from a single population in Melbourne, Victoria, Australia. Twenty-eight loci were polymorphic with observed heterozygosity ranging from 0.03 to 0.96 (mean 0.58) and the number of alleles per locus ranged from 2 to 18 (average of 6.5); we confirmed that 24 loci conformed to Hardy–Weinberg expectations. The 24 loci identified here will be sufficient to unequivocally identify individuals and will be useful in understanding the reproductive ecology, population genetics and the gene flow amongst localities in urban environments where this bird thrives.
Additional keywords: 454 GS-FLX, nocturnal, shotgun sequencing, urban.
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