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Evolutionary, molecular and comparative zoology
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RESEARCH ARTICLE
Contents Vol 54(4)

A molecular phylogeny of the Australian monitor lizards (Squamata : Varanidae) inferred from mitochondrial DNA sequences

A. J. Fitch A B C , A. E. Goodman A and S. C. Donnellan B
+ Author Affiliations
- Author Affiliations

A School of Biological Sciences, Flinders University, GPO Box 2100, SA 5001, Australia.

B Evolutionary Biology Unit and Centre for Evolutionary Biology and Biodiversity, South Australian Museum, North Terrace, Adelaide, SA 5000, Australia.

C Corresponding author. Email: alison.fitch@flinders.edu.au

Australian Journal of Zoology 54(4) 253-269 https://doi.org/10.1071/ZO05038
Submitted: 11 July 2005  Accepted: 9 May 2006   Published: 11 August 2006

Abstract

To date no complete phylogeny of all of the currently recognised Indo-Australian varanid species and subspecies has been published. This paper presents a comprehensive mitochondrial gene phylogeny of these lizards. A portion of the mitochondrial genome comprising part of the ND4 gene and three adjacent tRNA genes (hereafter referred to as ND4) was analysed alone and, for a subset of the taxa, combined with previously published mitochondrial data. Similar tree topologies were produced by both datasets although combining the data helped resolve some of the unresolved or weakly supported nodes in the ND4 analyses. The monophyly of the Indo-Australian group was strongly supported in all analyses. This group comprised three major lineages: the gouldii group, the Odatria group and the varius group. Mitochondrial ND4 nucleotide sequences were successfully amplified from all of the Indo-Australian monitor species and subspecies currently recognised and, as such, is the first comprehensive phylogenetic study of the Australian monitor lizards published. Analysis of the tempo of diversification and evolution of preferred habitat use identified six episodes of increased net speciation rate, with two closely adjacent episodes showing the highest rates of diversification and correlating with the appearance of all preferred habitat types. The comprehensive molecular phylogenetic framework will also be useful for the identification of varanid species and traded products derived from monitors and, as such, has important applications for wildlife management and conservation.


Acknowledgments

We thank G. Armstrong, J. Armstrong, M. Bonnett, P. Canty, A. de Chambrier, T. Chesser, R. Foster, C. Grant, D. Holland, P. Horner, R. How, M. Hutchinson, R. Leys, B. Maryan, T. Reardon, S. Richards, R. Sadlier, A. Skinner, S. Smith and S. Sweet for kindly collecting and/or donating tissues. Additionally, we thank A. Skinner and two anonymous referees for comments on the manuscript. This work was funded in part by a South Australian Department for Environment and Heritage Wildlife Conservation Fund grant and the Flinders University. AJF was supported by a Flinders University Research award.


References

Arévalo, E. , Davis, S. K. , and Sites, J. (1994). Mitochondrial DNA sequence divergence and phylogenetic relationships among eight chromosome races of the Sceloporus grammicus complex (Phrynosomatidae) in Central Mexico. Systematic Biology 43, 387–418.
| Crossref | GoogleScholarGoogle Scholar | Cogger H. G. (2000). ‘Reptiles and Amphibians of Australia.’ (Reed New Holland: Sydney.)

Comstock, K. E. , Ostrander, E. A. , and Wasser, S. K. (2003). Amplifying nuclear and mitochondrial DNA from African elephant ivory: a tool for monitoring the ivory trade. Conservation Biology 17, 1840–1843.
| Crossref | GoogleScholarGoogle Scholar | De Lisle H. F. (1996). ‘The Natural History of Monitor Lizards.’ (Krieger Publishing Company: Malabar.)

Donnellan, S. C. , Hutchinson, M. N. , and Saint, K. M. (1999). Molecular evidence for the phylogeny of Australian gekkonoid lizards. Biological Journal of the Linnean Society 67, 97–118.
| Crossref | GoogleScholarGoogle Scholar | Eriksson T. (1998). ‘AutoDecay version 4.0.’ Program distributed by the author. (Bergius Foundation, Royal Swedish Academy of Sciences: Stockholm.)

Estes R. (1983). ‘Handbuch der Paläoherpetologie. Teil 10A, Sauria terrestria, Amphisbaenia.’ (Gustav Fischer Verlag: Stuttgart.)

Estes, R. (1984). Fish, amphibians and reptiles from the Etadunna Formation, Miocene of South Australia. Australian Zoologist 21, 335–343.
Jennings W. B., and Pianka E. R. (2004). Tempo and timing of the Australian Varanus radiation. In ‘Varanoid Lizards of the World’. (Eds E. R. Pianka, D. R. King and R. A. King.) pp. 77–87. (Indiana University Press: Bloomington, IL.)

Jukes T. H., and Cantor C. R. (1969). Evolution of protein molecules. In ‘Mammalian Protein Metabolism’. (Ed. H. N. Munro.) pp. 21–132. (Academic Press: New York.)

Kasper, M. L. , Reeson, A. F. , Cooper, S. J. B. , Perry, K. D. , and Austin, A. D. (2004). Assessment of prey overlap between a native (Polistes humilis) and an introduced (Vespula germanica) social wasp using morphology and phylogenetic analyses of 16S rDNA. Molecular Ecology 13, 2037–2048.
| Crossref | GoogleScholarGoogle Scholar | PubMed | King D., and Green B. (1999). ‘Goannas. The Biology of Varanid Lizards.’ (University of New South Wales Press: Sydney.)

King, D. , King, M. , and Baverstock, P. (1991). A new phylogeny of the Varanidae. Mertensiella 2, 211–219.
King M. (1990). Chromosomal and immunogenetic data: a new perspective on the origin of Australia’s reptiles. In ‘Cytogenetics of Amphibians and Reptiles. Advances in Life Sciences’. (Ed. E. Olmo.) pp. 153–180. (Birkhauser Verlag: Basel.)

King, M. , and King, D. (1975). Chromosomal evolution in the lizard genus Varanus (Reptilia). Australian Journal of Biological Sciences 28, 89–108.
| PubMed | Maddison W. P., and Maddison D. R. (1992). ‘MacClade. Analysis of Phylogeny and Character Evolution.’ (Sinauer: Sunderland, MA.)

Mallet, J. , and Willmott, K. (2003). Taxonomy: renaissance or Tower of Babel? Trends in Ecology & Evolution 18, 57–59.
| Crossref | GoogleScholarGoogle Scholar | Molnar R. E. (2004). The long and honorable history of monitors and their kin. In ‘Varanoid Lizards of the World’. (Eds E. R. Pianka, D. R. King and R. A. King.) pp. 10–67. (Indiana University Press: Bloomington, IL.)

Momen, H. (2002). Molecular taxonomy of trypanosomatids: some problems and pitfalls. Archives of Medical Research 33, 413–415.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Palumbi S., Martin A., Romano S., McMillan W. O., Stice L., and Grabowski G. (1991). ‘The Simple Fool’s Guide to PCR Version 2.’ (Department of Zoology and Kewalo Marine Laboratory, University of Hawaii: Honolulu, HI.)

Pepin, D. J. (1999). The origin of monitor lizards based on a review of the fossil evidence. Mertensiella 11, 11–42.
Pianka E. R., King D. R., and King R. A. (2004). ‘Varanoid Lizards of the World.’ (Indiana University Press: Bloomington, IL.)

Posada, D. , and Crandall, K. A. (1998). Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.
| Crossref | GoogleScholarGoogle Scholar | PubMed | Rambaut A. (1995). ‘Se-Al. Sequence Alignment Editor.’ (Evolutionary Biology Unit, University of Oxford: Oxford, UK.) Available at http://evolve.zoo.ox.ac.uk/software.html?id=seal [Verified 10 July 2006].

Rambaut, A. , Harvey, P. H. , and Nee, S. (1997). End-Epi: an application for inferring phylogenetic and population dynamical processes from molecular sequences. Computer Applications in the Biosciences 13, 303–306.
| PubMed | Schluter D. (2000). ‘The Ecology of Adaptive Radiation.’ (Oxford University Press: Oxford.)

Shine, R. , Harlow, P. S. , Keogh, J. S. , and Boeadi, (1996). Commercial harvesting of giant lizards: the biology of water monitors Varanus salvator in southern Sumatra. Biological Conservation 77, 125–134.
| Crossref | GoogleScholarGoogle Scholar | Steel R. (1997). ‘Living Dragons. A Natural History of the World’s Monitor Lizards.’ (RD Press: Sydney.)

Swofford D. L. (2002). ‘PAUP*. Phylogenetic Analysis Using Parsimony (*And Other Methods).’ (Sinauer: Sunderland, MA.)

Takeyama, H. , Tsuzuki, H. , Chow, S. , Nakayama, H. , and Matsunaga, T. (2000). Discrimination between Atlantic and Pacific subspecies of northern bluefin tuna (Thunnus thynnus) by magnetic-capture hybridization using bacterial magnetic probes. Marine Biotechnology 2, 309–313.
| PubMed | TRAFFIC International (2003). ‘What are the main wildlife commodities in trade? 25 years of TRAFFIC.’ Available at http://www.traffic.org/25/wild4_4.htm [Verified August 2003].

Vidal, N. , and Hedges, S. B. (2004). Molecular evidence for a terrestrial origin of snakes. Proceedings of the Royal Society of London. Series B. Biological Sciences 271(Suppl.), S226–S229.
Vincent M., and Wilson S. (1999). ‘Australian Goannas.’ (New Holland Publishers: Sydney.)

Vincent, S. , Vian, J. M. , and Carlotti, M. P. (2000). Partial sequencing of the cytochrome oxydase b subunit gene 1: a tool for the identification of European species of blow flies for postmortem interval estimation. Journal of Forensic Sciences 45, 820–823.
| PubMed |

Welter, C. , Dooley, S. , and Blin, N. (1989). A rapid protocol for the purification of mitochondrial DNA suitable for studying restriction fragment length polymorphisms. Gene 83, 169–172.
| Crossref | GoogleScholarGoogle Scholar | PubMed |

Wetton, J. H. , Tsang, C. S. F. , Roney, C. A. , and Spriggs, A. C. (2004). An extremely sensitive species-specific ARMs PCR test for the presence of tiger bone DNA. Forensic Science International 140, 139–145.
| Crossref | GoogleScholarGoogle Scholar | PubMed |

Wilson, E. O. , and Brown, W. L. (1953). The subspecies concept and its taxonomic application. Systematic Zoology 2, 97–111.
| Crossref | GoogleScholarGoogle Scholar |

Zhang, D.-X. , and Hewitt, G. M. (1996). Nuclear integrations: challenges for mitochondrial DNA markers. Trends in Ecology & Evolution 11, 247–251.
| Crossref | GoogleScholarGoogle Scholar |

Ziegler, T. , and Böhme, W. (1999). Genital morphology and systematics of two recently described monitor lizards of the Varanus (Euprepiosaurus) indicus group. Mertensiella 11, 121–128.


Ziegler, T. , Böhme, W. , Eidenmüller, B. , and Philipp, K. M. (2001). A note on the coexistence of three species of Pacific monitor lizards in Australia (Sauria, Varanidae, Varanus indicus group). Bonner Zoologische Beiträge, Bonn 50, 27–30.