Soil biochemical activities after the application of pyroligneous acid to soil
Roberto Cardelli
A B , Michelangelo Becagli A , Fausto Marchini A and Alessandro Saviozzi A
+ Author Affiliations
- Author Affiliations
A Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto, 80, 56124 Pisa, Italy.
B Corresponding author. Email address: roberto.cardelli@unipi.it
Soil Research 58(5) 461-467 https://doi.org/10.1071/SR19373
Submitted: 16 December 2019 Accepted: 10 March 2020 Published: 22 April 2020
Abstract
Pyroligneous acid (PA) is produced during the combustion of woody biomass and is a complex aqueous fraction resulting from the thermochemical rupture of the components of vegetable biomass. We evaluated the effect of PA on the soil microbial community and activity in order to assess the applicability of this acid in soil and to gather further information on the mechanisms of its toxicity or stimulation. Five concentrations of PA solution (0, 0.5, 1, 2 and 5%) were selected to monitor the biochemical parameters of the soil. The respirometric test showed that the increase in the evolved carbon dioxide-carbon (C) was not due to a release of the native organic C from the soil, but only from the organic compounds of PA. The highest values of microbial biomass content were found in the soil treated with the lowest PA doses, but decreased with increased doses. At higher application doses (2 and 5%), there was a decrease in most enzymatic activities and a loss of soil quality. When PA was applied in doses of up to 1%, our results indicated no negative effects on soil biology and that there was even an improvement.
Additional keywords: soil enzymes, soil microbial biomass, soil respiration, wood vinegar.
References
Anderson TH, Domsch KH (1993) The metabolic quotient for CO2 (qCO2) a specific activity parameter to assess the effect of environmental conditions, such as pH, on the microbial biomass of forest soils. Soil Biology & Biochemistry 25, 393–395.| The metabolic quotient for CO2 (qCO2) a specific activity parameter to assess the effect of environmental conditions, such as pH, on the microbial biomass of forest soils.Crossref | GoogleScholarGoogle Scholar |
Balat M, Balat M, Kırtay E, Balat H (2009) Main routes for the thermoconversion of biomass into fuels and chemicals. Part 1: pyrolysis systems. Energy Conversion and Management 50, 3147–3157.
| Main routes for the thermoconversion of biomass into fuels and chemicals. Part 1: pyrolysis systems.Crossref | GoogleScholarGoogle Scholar |
Beck T, Bengel A (1992) Die mikrobielle Biomasse in Böden, Teil I: Ihre Bestimmung, Indikatorfunktion und Bedeutung für die Stoffumsetzungen. SuB Heft 01/92, III-6-III-10.
Denevan WM (1996) A bluff model of riverine settlement in prehistoric Amazonia. Annals of the Association of American Geographers 86, 654–681.
Benzon HRL, Rubenecia MRU, Ultra VU, Lee SC (2015) Chemical and biological properties of paddy soil treated with herbicides and pyroligneous acid. The Journal of Agricultural Science 7, 20–29.
Cardelli R, Becagli M, Marchini F, Saviozzi A (2016) Short-term releases of CO2 from newly mixed biochar and calcareous soil Soil Use and Management 32, 543–545.
| Short-term releases of CO2 from newly mixed biochar and calcareous soilCrossref | GoogleScholarGoogle Scholar |
Dick RP, Breakwell DP, Turco RF (1996) Soil enzyme activities and biodiversity measurements as integrative microbiological indicators. In ‘Methods for Assessing Soil Quality’. (Eds JW Doran, AJ Jones) pp. 247–271. (SSSA: Madison WI, USA)
Du W, Zhu Y, Zhang X, Geng Y, Lin P (2016) Effects of wood vinegar on microbial biomasses and enzyme activity in sandy soil. Shuitu Baochi Tongbao 3, 358–368.
Eivazi F, Tabatabai MA (1977) Phosphatases in soil. Soil Biology & Biochemistry 9, 167–172.
| Phosphatases in soil.Crossref | GoogleScholarGoogle Scholar |
Eivazi F, Tabatabai MA (1988) Glucosidases and galactosidases in soils. Soil Biology & Biochemistry 20, 601–606.
| Glucosidases and galactosidases in soils.Crossref | GoogleScholarGoogle Scholar |
FAO (2015) ‘Global Forest Resources Assessment 2015.’ (Food and Agriculture Organization of the United Nation: Rome)
García-Ruiz R, Ochoa V, Hinojosa MB, Carreira JA (2008) Suitability of enzyme activities for the monitoring of soil quality improvement in organic agricultural systems. Soil Biology & Biochemistry 40, 2137–2145.
| Suitability of enzyme activities for the monitoring of soil quality improvement in organic agricultural systems.Crossref | GoogleScholarGoogle Scholar |
Grewal A, Abbey L, Gunupuru LR (2018) Production, prospects and potential application of pyroligneous acid in agriculture. Journal of Analytical and Applied Pyrolysis 135, 152–159.
| Production, prospects and potential application of pyroligneous acid in agriculture.Crossref | GoogleScholarGoogle Scholar |
Islam KR, Weil RR (2000) Land use effects on soil quality in a tropical forest ecosystem of Bangladesh. Agriculture, Ecosystems & Environment 79, 9–16.
| Land use effects on soil quality in a tropical forest ecosystem of Bangladesh.Crossref | GoogleScholarGoogle Scholar |
Jin K, Sleutel S, Buchan D, De Neve S, Cai DX, Gabriels D, Jin JY (2009) Changes of soil enzyme activities under different tillage practices in the Chinese Loess Plateau. Soil & Tillage Research 104, 115–120.
| Changes of soil enzyme activities under different tillage practices in the Chinese Loess Plateau.Crossref | GoogleScholarGoogle Scholar |
Kandeler E, Gerber H (1988) Short-term assay of soil urease activity using colorimetric determination of ammonium. Biology and Fertility of Soils 6, 68–72.
| Short-term assay of soil urease activity using colorimetric determination of ammonium.Crossref | GoogleScholarGoogle Scholar |
Kang MY, Heo KH, Kim JH, Cho SS, Seo PD, Ric CM, Lee SC (2012) Effects of carbonized rice hull and wood charcoal mixed with pyroligneous acid on the yield, and antioxidant and nutritional quality of rice. Turkish Journal of Agriculture and Forestry 36, 45–53.
Koc I, Yardim EN, Akca MO, Namli A (2018) Impact of pesticides and wood vinegar, used in wheat agro-ecosystems, on the soil enzyme activities. Fresenius Environmental Bulletin 27, 2442–2448.
Koc I, Ogun E, Namli A, Mendes M, Kutlu E, Yardim EN (2019) The effects of wood vinegar on some soil microorganisms. Applied Ecology and Environmental Research 17, 2437–2447.
| The effects of wood vinegar on some soil microorganisms.Crossref | GoogleScholarGoogle Scholar |
Lee CS, Yi EH, Kim HR, Huh SR, Sung SH, Chung MH, Ye SK (2011) Anti-dermatitis effects of oak wood vinegar on the DNCB-induced contact hypersensitivity via STAT3 suppression. Journal of Ethnopharmacology 135, 747–753.
| Anti-dermatitis effects of oak wood vinegar on the DNCB-induced contact hypersensitivity via STAT3 suppression.Crossref | GoogleScholarGoogle Scholar | 21511023PubMed |
Levi-Minzi R, Riffaldi R, Saviozzi A (1990) Carbon mineralization in soil amended with different organic materials. Agriculture, Ecosystems & Environment 31, 325–335.
| Carbon mineralization in soil amended with different organic materials.Crossref | GoogleScholarGoogle Scholar |
Lu H, Lashari MS, Liu X, Ji H, Li L, Zheng J, Kibue GW, Joseph S, Pan G (2015) Changes in soil microbial community structure and enzyme activity with amendment of biochar-manure compost and pyroligneous solution in a saline soil from Central China. European Journal of Soil Biology 70, 67–76.
| Changes in soil microbial community structure and enzyme activity with amendment of biochar-manure compost and pyroligneous solution in a saline soil from Central China.Crossref | GoogleScholarGoogle Scholar |
Mansur D, Yoshikawa T, Norinaga K, Hayashi J, Tago T, Masuda T (2013) Production of ketones from pyroligneous acid of woody biomass pyrolysis over an iron-oxide catalyst. Fuel 103, 130–134.
| Production of ketones from pyroligneous acid of woody biomass pyrolysis over an iron-oxide catalyst.Crossref | GoogleScholarGoogle Scholar |
Mathew S, Zakaria ZA (2015) Pyroligneous acid - the smoky acidic liquid from plant biomass. Applied Microbiology and Biotechnology 99, 611–622.
| Pyroligneous acid - the smoky acidic liquid from plant biomass.Crossref | GoogleScholarGoogle Scholar | 25467926PubMed |
Mathew S, Zakaria ZA, Musa F, Ngadiran S, Suan CL (2014) Free radical scavenging property and chemical profile of pyroligneous acid from pineapple waste biomass. Presented at the 5th International Conference on Wellbeing for Biotechnology, 11–12 June, UTM Kuala Lumpur, Malaysia. pp. 1985–1992.
Mmojieje J, Hornung A (2015) The potential application of pyroligneous acid in the UK agricultural industry. Journal of Crop Improvement 29, 228–246.
| The potential application of pyroligneous acid in the UK agricultural industry.Crossref | GoogleScholarGoogle Scholar |
Nsamibana D, Haynes RT, Wallis FM (2004) Size, activity and catabolic diversity of the soil microbial biomass as affected by land use. Applied Soil Ecology 2, 81–92.
Oramahi HA, Yoshimura T, Diba F, Setyawati D (2018) Antifungal and antitermitic activities of wood vinegar from oil palm trunk. Journal of Wood Science 64, 311–317.
| Antifungal and antitermitic activities of wood vinegar from oil palm trunk.Crossref | GoogleScholarGoogle Scholar |
Paz-Ferreiro J, Fu S (2016) Biological indices for soil quality evaluation: perspectives and limitations. Land Degradation & Development 27, 14–25.
| Biological indices for soil quality evaluation: perspectives and limitations.Crossref | GoogleScholarGoogle Scholar |
Paz-Ferreiro J, Gascó G, Gutiérrez B, Méndez A (2012) Soil biochemical activities and the geometric mean of enzyme activities after application of sewage sludge and sewage sludge biochar to soil. Biology and Fertility of Soils 48, 511–517.
| Soil biochemical activities and the geometric mean of enzyme activities after application of sewage sludge and sewage sludge biochar to soil.Crossref | GoogleScholarGoogle Scholar |
Prasertsit K, Rattanawan N, Ratanapisit J (2011) Effects of wood vinegar as an additive for natural rubber products. Songklanakarin Journal of Science and Technology 33, 425–430.
Rui Z, Wei D, Zhibin Y, Chao Z, Xiaojuan A (2014) Effects of wood vinegar on the soil microbial characteristics. Journal of Chemical and Pharmaceutical Research 6, 1254–1260.
Schnurer J, Rosswall T (1982) Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter. Applied and Environmental Microbiology 43, 1256–1261.
| Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter.Crossref | GoogleScholarGoogle Scholar | 16346026PubMed |
Schnurer J, Clarholm M, Rosswall T (1985) Microbial biomass and activity in an agricultural soil with different organic matter contents. Soil Biology & Biochemistry 17, 611–618.
| Microbial biomass and activity in an agricultural soil with different organic matter contents.Crossref | GoogleScholarGoogle Scholar |
SISS [Societa’ italiana Scienza del Suolo] (1995) ‘Metodi Normalizzati di Analisi del Suolo.’ (Edagricole: Bologna, Italy)
Souza JBG, Re-Poppia N, Raposo JL (2012) Characterization of pyroligneous acid used in agriculture by gas chromatography-mass spectrometry. Journal of the Brazilian Chemical Society 23, 610–617.
Steel RGD, Torrie JH, Dickey DA (1997) ‘Principles and Procedures of Statistics.’ (McGraw Hill: New York)
Steiner C, Teixeira WG, Lehmann J, Zech W (2004) Microbial response to charcoal amendments of highly weathered soils and Amazonian dark earths in central Amazonia-preliminary results. In ‘Amazonian Dark Earths: Explorations in Space and Time’. (Ed. WI Woods) pp. 195–212. (Springer: Heidelberg)
Sun H, Feng Y, Xue L, Mandal S, Wang H, Shi W, Yang L (2020) Responses of ammonia volatilization from rice paddy soil to application of wood vinegar alone or combined with biochar. Chemosphere 242, 125247
| Responses of ammonia volatilization from rice paddy soil to application of wood vinegar alone or combined with biochar.Crossref | GoogleScholarGoogle Scholar | 31896173PubMed |
Tabatabai M (1994) Soil enzymes. Methods of soil analysis Part 2: microbiological and biochemical properties SSSA Book Series 5. 2, 775–833.
Tabatabai MA, Bremner JM (1970) Arylsulfatase activity of soils. Soil Science Society of America Proceedings 34, 225–229.
| Arylsulfatase activity of soils.Crossref | GoogleScholarGoogle Scholar |
Vance ED, Brookes C, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass carbon. Soil Biology & Biochemistry 19, 703–707.
| An extraction method for measuring soil microbial biomass carbon.Crossref | GoogleScholarGoogle Scholar |
Zulkarami B, Ashrafuzzaman M, Husni MO, Ismail MR (2011) Effect of pyroligneous acid on growth, yield and quality improvement of rockmelon in soilless culture. Australian Journal of Crop Science 5, 1508–1514.
