The nitrification inhibitor 3,4,-dimethylpyrazole phosphate strongly inhibits nitrification in coarse-grained soils containing a low abundance of nitrifying microbiota
Elliott G. Duncan A B E , Cathryn A. O’Sullivan A , Anna K. Simonsen C , Margaret M. Roper A , Mark B. Peoples D , Karen Treble A and Kelley Whisson A
+ Author Affiliations
- Author Affiliations
A CSIRO Agriculture and Food, Centre for Environment and Life Sciences, 147 Underwood Avenue, Floreat, WA 6014, Australia.
B Present address: Future Industries Institute, University of South Australia, Mawson Lakes Boulevard, Mawson Lakes, SA 5095, Australia.
C CSIRO Land and Water, Centre for Environment and Life Sciences, 147 Underwood Avenue, Floreat, WA 6014, Australia.
D CSIRO Agriculture and Food, GPO Box 1700, Canberra, ACT 2601, Australia.
E Corresponding author. Email: Elliott.Duncan@unisa.edu.au
Soil Research 55(1) 28-37 https://doi.org/10.1071/SR15359
Submitted: 7 December 2015 Accepted: 12 February 2016 Published: 22 August 2016
Abstract
The effectiveness of the nitrification inhibitor 3,4,-dimethylpyrazole phosphate (DMPP) on sandy soils containing low nitrifying microbial abundance has not been established. Two coarse-grained soils, representative of Western Australia’s agricultural zones, were incubated with 100 mg N kg–1 soil, added as either urea, urea + DMPP or urea + nitrapyrin as an alternative nitrification inhibitor for comparative purposes. Ammonium (NH4+) and nitrate (NO3–) concentrations, potential nitrification rates (PNR) and the abundance of ammonia-oxidising bacteria (AOB) and archaea (AOA) were measured over time. Interactions between soil type and inhibitor type altered the extent of nitrification observed in these soils. When N was supplied as urea alone, NH4+-N concentrations decreased from 100 mg N kg–1 soil to approximately 20 mg N kg–1 soil in the high nutrient soil (Williams) and approximately 60 mg N kg–1 soil in the low nutrient soil (Vasse). These differences were reflected in AOB abundance, which was higher (~105 gene copies g–1 soil) in Williams soil than in Vasse soil (<104 gene copies g–1 soil). This difference could have been attributable to differences in soil pH between Williams and Vasse (5.4 vs 4.0 respectively) and/or copper (Cu) availability (~1.5 vs ~0.5 mg Cu kg–1 soil respectively), both of which have been demonstrated to reduce AOB abundance or limit nitrification. On the Williams soil, DMPP limited nitrification, resulting in approximately 80 mg N kg–1 soil being retained as NH4+-N. Nitrapyrin was similarly effective for the first 56 days of incubation, but declined considerably in effectiveness between Days 56 and 100. Changes in soil nitrification rates were accompanied by changes in AOB abundance, which was below 103 gene copies g–1 soil when nitrification was impaired. Both DMPP and nitrapyrin inhibit nitrification via chelating Cu and, because these soils contained low Cu concentrations, it may be possible that interactions between DMPP, naturally low abundance of AOB and low Cu availability facilitated the long-term inhibition of nitrification in these soils.
Additional keywords: ammonia-oxidising archaea (AOA), ammonia-oxidising bacteria (AOB), ammonium, mineral nitrogen, nitrapyrin, nitrate, potential nitrification rates (PNR).
References
Amberger A (1986) Potentials of nitrification inhibitors in modern N‐fertilizer management. Journal of Plant Nutrition and Soil Science 149, 469–484.| Potentials of nitrification inhibitors in modern N‐fertilizer management.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL28Xltlart7s%3D&md5=e2ee99f2b06cab5345847a15c7e121a5CAS |
Amberger A, Germann-Bauer M (1990) Effect of the nitrification inhibitors 1-amidino-2-thiourea and dicyandiamide in combination with urea and ammonium sulfate. Fertilizer Research 21, 179–183.
| Effect of the nitrification inhibitors 1-amidino-2-thiourea and dicyandiamide in combination with urea and ammonium sulfate.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXkslOitrc%3D&md5=a256fba1905942142081d338ca2cdadbCAS |
Azam F, Benckiser G, Müller C, Ottow J (2001) Release, movement and recovery of 3,4-dimethylpyrazole phosphate (DMPP), ammonium, and nitrate from stabilized nitrogen fertilizer granules in a silty clay soil under laboratory conditions. Biology and Fertility of Soils 34, 118–125.
| Release, movement and recovery of 3,4-dimethylpyrazole phosphate (DMPP), ammonium, and nitrate from stabilized nitrogen fertilizer granules in a silty clay soil under laboratory conditions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXltlyqtL8%3D&md5=d4d79715f26a32dd6f9be9949de64699CAS |
Barth G, Von Tucher S, Schmidhalter U (2001) Influence of soil parameters on the effect of 3,4-dimethylpyrazole-phosphate as a nitrification inhibitor. Biology and Fertility of Soils 34, 98–102.
| Influence of soil parameters on the effect of 3,4-dimethylpyrazole-phosphate as a nitrification inhibitor.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXltlyqt7Y%3D&md5=c0c19b1ef5f6c3ea8612e3a5468c3a0bCAS |
Broos K, Warne MSJ, Heemsbergen DA, Stevens D, Barnes MB, Correll RL, McLaughlin MJ (2007) Soil factors controlling the toxicity of copper and zinc to microbial processes in Australian soils. Environmental Toxicology and Chemistry 26, 583–590.
| Soil factors controlling the toxicity of copper and zinc to microbial processes in Australian soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXkt1Cnur8%3D&md5=d07f143e782621b1a88a031af46d4e03CAS | 17447541PubMed |
Carrasco I, Villar JM (2001) Field evaluation of DMPP as a nitrification inhibitor in the area irrigated by the Canal d’Urgell (Northeast Spain). In ‘Plant nutrition: food security and sustainability of agro-ecosystems through basic and applied research’. (Eds WJ Horst, MK Schenk, A Bürkert, N Claassen, H Flessa, WB Frommer, H Goldbach, H-W Olfs, V Römheld, B Sattelmacher, U Schmidhalter, S Schubert, Nv Wirén, L Wittenmayer) pp. 764–765. (Springer Netherlands: Dordrecht)
Chaves B, Opoku A, De Neve S, Boeckx P, Van Cleemput O, Hofman G (2006) Influence of DCD and DMPP on soil N dynamics after incorporation of vegetable crop residues. Biology and Fertility of Soils 43, 62–68.
| Influence of DCD and DMPP on soil N dynamics after incorporation of vegetable crop residues.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XosFWktro%3D&md5=05304825ebf7401cd345e22a9a9dae69CAS |
Chen D, Suter H, Islam A, Edis R, Freney J, Walker C (2008) Prospects of improving efficiency of fertiliser nitrogen in Australian agriculture: a review of enhanced efficiency fertilisers. Soil Research 46, 289–301.
| Prospects of improving efficiency of fertiliser nitrogen in Australian agriculture: a review of enhanced efficiency fertilisers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXns1OktLw%3D&md5=fec61f7dd15fe4ea2a2bc5ea7113200cCAS |
Chen Q, Qi L, Bi Q, Dai P, Sun D, Sun C, Liu W, Lu L, Ni W, Lin X (2015) Comparative effects of 3,4-dimethylpyrazole phosphate (DMPP) and dicyandiamide (DCD) on ammonia-oxidizing bacteria and archaea in a vegetable soil. Applied Microbiology and Biotechnology 99, 477–487.
| Comparative effects of 3,4-dimethylpyrazole phosphate (DMPP) and dicyandiamide (DCD) on ammonia-oxidizing bacteria and archaea in a vegetable soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhsVKmsbrK&md5=c7dd51e89259faa72c5885b55bbfaf58CAS | 25172135PubMed |
Crawford D, Chalk P (1993) Sources of N uptake by wheat (Triticum aestivum L.) and N transformations in soil treated with a nitrification inhibitor (nitrapyrin). Plant and Soil 149, 59–72.
| Sources of N uptake by wheat (Triticum aestivum L.) and N transformations in soil treated with a nitrification inhibitor (nitrapyrin).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXktVOqsL8%3D&md5=47197216589629b5491e0e656502780cCAS |
Crews TE, Peoples MB (2004) Legume versus fertilizer sources of nitrogen: ecological tradeoffs and human needs. Agriculture, Ecosystems & Environment 102, 279–297.
| Legume versus fertilizer sources of nitrogen: ecological tradeoffs and human needs.Crossref | GoogleScholarGoogle Scholar |
Dalal RC, Wang W, Robertson GP, Parton WJ (2003) Nitrous oxide emission from Australian agricultural lands and mitigation options: a review. Soil Research 41, 165–195.
| Nitrous oxide emission from Australian agricultural lands and mitigation options: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXktFKisr8%3D&md5=82cb3d74aaae67b11972b27b0ea300f6CAS |
Di H, Cameron K (2002) The use of a nitrification inhibitor, dicyandiamide (DCD), to decrease nitrate leaching and nitrous oxide emissions in a simulated grazed and irrigated grassland. Soil Use and Management 18, 395–403.
| The use of a nitrification inhibitor, dicyandiamide (DCD), to decrease nitrate leaching and nitrous oxide emissions in a simulated grazed and irrigated grassland.Crossref | GoogleScholarGoogle Scholar |
Di H, Cameron K (2004) Effects of temperature and application rate of a nitrification inhibitor, dicyandiamide (DCD), on nitrification rate and microbial biomass in a grazed pasture soil. Soil Research 42, 927–932.
| Effects of temperature and application rate of a nitrification inhibitor, dicyandiamide (DCD), on nitrification rate and microbial biomass in a grazed pasture soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhtVOqsLnF&md5=9407b3bc4e533d19ff75495f9a3413ecCAS |
Di H, Cameron K, Sherlock R (2007) Comparison of the effectiveness of a nitrification inhibitor, dicyandiamide, in reducing nitrous oxide emissions in four different soils under different climatic and management conditions. Soil Use and Management 23, 1–9.
| Comparison of the effectiveness of a nitrification inhibitor, dicyandiamide, in reducing nitrous oxide emissions in four different soils under different climatic and management conditions.Crossref | GoogleScholarGoogle Scholar |
Díez López JA, Hernaiz P (2008) Effect of a nitrification inhibitor (DMPP) [3,4-dimethylpyrazole phosphate] on nitrate leaching and maize yield during two growing seasons. Spanish Journal of Agricultural Research 6, 294–303.
| Effect of a nitrification inhibitor (DMPP) [3,4-dimethylpyrazole phosphate] on nitrate leaching and maize yield during two growing seasons.Crossref | GoogleScholarGoogle Scholar |
Fillery I, McInnes K (1992) Components of the fertiliser nitrogen balance for wheat production on duplex soils. Animal Production Science 32, 887–899.
| Components of the fertiliser nitrogen balance for wheat production on duplex soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXkslWmtrY%3D&md5=5c5ff2d58b7c531ea777aab19cc4069cCAS |
Fischer T, Byerlee D, Edmeades G (2012) Crop yields and food security: will yield increases continue to feed the world. In ‘Proceedings of the 12th Australian Agronomy Conference’, 14–18 October, 2012, Armidale, NSW. (Ed. I Yunusa). Available at http://www.regional.org.au/au/asa/2012/agriculture/7936_fischerra.htm [verified 1 December 2015].
Foulds W (1993) Nutrient concentrations of foliage and soil in south‐western Australia. New Phytologist 125, 529–546.
| Nutrient concentrations of foliage and soil in south‐western Australia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXisVentr8%3D&md5=01cb57849b03b2a385c06619621f72e2CAS |
Francis CA, Roberts KJ, Beman JM, Santoro AE, Oakley BB (2005) Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proceedings of the National Academy of Sciences of the United States of America 102, 14683–14688.
| Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtFKjsb7J&md5=e905063970ee0d2bbb2aff4987646ff6CAS | 16186488PubMed |
Gong P, Zhang L-L, Wu J-Z, Chen Z-H, Chen L-J (2013) Responses of ammonia-oxidizing bacteria and archaea in two agricultural soils to nitrification inhibitors DCD and DMPP: a pot experiment. Pedosphere 23, 729–739.
| Responses of ammonia-oxidizing bacteria and archaea in two agricultural soils to nitrification inhibitors DCD and DMPP: a pot experiment.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXjslersbY%3D&md5=98b2af297261cee33d9b1d7ef1a9a1a2CAS |
Hamid A, Ahmad M (1995) Interaction of 15N-labelled ammonium nitrate, urea and ammonium sulphate with soil N during growth of wheat (Triticum aestivum L.) under field conditions. Biology and Fertility of Soils 20, 185–189.
| Interaction of 15N-labelled ammonium nitrate, urea and ammonium sulphate with soil N during growth of wheat (Triticum aestivum L.) under field conditions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXosFehurc%3D&md5=8ceeb491c5fc16bcce97ac0ed8a05e09CAS |
Hart S, Stark JM, Davidson E, Firestone M (1994) Mineralization, immobilization, and nitrification. In ‘Methods of soil analysis. Part 2. Microbiological and biochemical properties. Vol. 5’. (Eds R Weaver, J Angle, B Bottomley) pp. 985–1018. (Soil Science Society of America: Madison, WI)
Hatch D, Trindade H, Cardenas L, Carneiro J, Hawkins J, Scholefield D, Chadwick D (2005) Laboratory study of the effects of two nitrification inhibitors on greenhouse gas emissions from a slurry-treated arable soil: impact of diurnal temperature cycle. Biology and Fertility of Soils 41, 225–232.
| Laboratory study of the effects of two nitrification inhibitors on greenhouse gas emissions from a slurry-treated arable soil: impact of diurnal temperature cycle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjtVWmtbw%3D&md5=5653cc0c7c2976d8569580529854674eCAS |
He J-z, Shen J-p, Zhang L-m, Zhu Y-g, Zheng Y-m, Xu M-g, Di H (2007) Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices. Environmental Microbiology 9, 2364–2374.
| Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtVyku7rE&md5=6eb8c0c0278e49cf2f5632ecf7a1718eCAS | 17686032PubMed |
Hoeft RG (1984) Current status of nitrification inhibitor use in U.S. Agriculture. In ‘Nitrogen in crop production’. (Ed. RD Hauck) pp. 561–570. (American Society of Agronomy, Crop Science Society of America, Soil Science Society of America: Madison, WI)
Huber D, Warren H, Nelson D, Tsai C (1977) Nitrification inhibitors: new tools for food production. Bioscience 27, 523–529.
| Nitrification inhibitors: new tools for food production.Crossref | GoogleScholarGoogle Scholar |
Irigoyen I, Muro J, Azpilikueta M, Aparicio-Tejo P, Lamsfus C (2003) Ammonium oxidation kinetics in the presence of nitrification inhibitors DCD and DMPP at various temperatures. Soil Research 41, 1177–1183.
| Ammonium oxidation kinetics in the presence of nitrification inhibitors DCD and DMPP at various temperatures.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXot1Cgt70%3D&md5=92d1b1fadc60b4d0482344e6dd14bd3cCAS |
Jenkinson D (2001) The impact of humans on the nitrogen cycle, with focus on temperate arable agriculture. Plant and Soil 228, 3–15.
| The impact of humans on the nitrogen cycle, with focus on temperate arable agriculture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXhtlWktbc%3D&md5=282ba544560b8d83051d21b713afcaf2CAS |
Keeney DR, Nelson D (1982) Nitrogen – inorganic forms. In ‘Methods of soil analysis. Part 2. Chemical and microbiological properties’. (Eds R Weaver, J Angle, B Bottomley) pp. 643–698. (Soil Science Society of America: Madison, WI)
Kidwaro FM, Kephart KD (1998) Retention of nitrogen from stabilized anhydrous ammonia in the soil profile during winter wheat production in Missouri. Communications in Soil Science and Plant Analysis 29, 481–499.
| Retention of nitrogen from stabilized anhydrous ammonia in the soil profile during winter wheat production in Missouri.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXisVWhsL4%3D&md5=33de5dbe5025eb47306add53c05fd483CAS |
Kleineidam K, Košmrlj K, Kublik S, Palmer I, Pfab H, Ruser R, Fiedler S, Schloter M (2011) Influence of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on ammonia-oxidizing bacteria and archaea in rhizosphere and bulk soil. Chemosphere 84, 182–186.
| Influence of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on ammonia-oxidizing bacteria and archaea in rhizosphere and bulk soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXmsVygu74%3D&md5=95a5ca25efeb7c45ddda0773396992cbCAS | 21435682PubMed |
Ladha JK, Pathak H, Krupnik TJ, Six J, van Kessel C (2005) Efficiency of fertilizer nitrogen in cereal production: retrospects and prospects. Advances in Agronomy 87, 85–156.
| Efficiency of fertilizer nitrogen in cereal production: retrospects and prospects.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXitlahsLg%3D&md5=e0f949b1a934047e4fd867c7ed8eabe2CAS |
Li H, Liang X, Chen Y, Lian Y, Tian G, Ni W (2008) Effect of nitrification inhibitor DMPP on nitrogen leaching, nitrifying organisms, and enzyme activities in a rice-oilseed rape cropping system. Journal of Environmental Sciences 20, 149–155.
| Effect of nitrification inhibitor DMPP on nitrogen leaching, nitrifying organisms, and enzyme activities in a rice-oilseed rape cropping system.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXisFSisrw%3D&md5=d9d88575c1c3131b78e75ad7180e8c15CAS |
Lindsay W, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of America Journal 42, 421–428.
| Development of a DTPA soil test for zinc, iron, manganese, and copper.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1cXltVKntLs%3D&md5=da950865572245a99ec0b569bd6264b8CAS |
Linzmeier W, Gutser R, Schmidhalter U (2001) Nitrous oxide emission from soil and from a nitrogen-15-labelled fertilizer with the new nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP). Biology and Fertility of Soils 34, 103–108.
| Nitrous oxide emission from soil and from a nitrogen-15-labelled fertilizer with the new nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXltlyqt7c%3D&md5=21711d3e685d6e48d1960feab869e19aCAS |
Maher W, Foster S, Krikowa F, Snitch P, Chapple G, Craig P (2001) Measurement of trace metals and phosphorus in marine animal and plant tissues by low volume microwave digestion and ICPMS. Journal of Analytical Atomic Spectrometry 22, 361–369.
Maher W, Krikowa F, Kirby J, Townsend A, Snitch P (2003) Measurement of trace elements in marine environmental samples using solution ICPMS. Current and future applications. Australian Journal of Chemistry 56, 103–116.
| Measurement of trace elements in marine environmental samples using solution ICPMS. Current and future applications.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXjslGnsLk%3D&md5=a48f6960c35f1d9b15680c6d65f98c08CAS |
McArthur WM (1991) ‘Reference soils of south-western Australia.’ (Department of Agriculture, Western Australia on behalf of the Australian Society of Soil Science: Perth, WA, Australia)
McCarty G (1999) Modes of action of nitrification inhibitors. Biology and Fertility of Soils 29, 1–9.
| Modes of action of nitrification inhibitors.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXitVKksr0%3D&md5=46f79289a4b663cc1f114cc9cf17c23dCAS |
O’Sullivan CA, Wakelin SA, Fillery IRP, Gregg AL, Roper MM (2011) Archaeal ammonia oxidisers are abundant in acidic, coarse-textured Australian soils. Soil Research 49, 715–724.
| Archaeal ammonia oxidisers are abundant in acidic, coarse-textured Australian soils.Crossref | GoogleScholarGoogle Scholar |
O’Sullivan CA, Wakelin SA, Fillery IRP, Roper MM (2013) Factors affecting ammonia-oxidising microorganisms and potential nitrification rates in southern Australian agricultural soils. Soil Research 51, 240–252.
| Factors affecting ammonia-oxidising microorganisms and potential nitrification rates in southern Australian agricultural soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtVWmur7M&md5=19afe31aee1439fa251bf857839cdfedCAS |
Pasda G, Hähndel R, Zerulla W (2001) Effect of fertilizers with the new nitrification inhibitor DMPP (3,4-dimethylpyrazole phosphate) on yield and quality of agricultural and horticultural crops. Biology and Fertility of Soils 34, 85–97.
| Effect of fertilizers with the new nitrification inhibitor DMPP (3,4-dimethylpyrazole phosphate) on yield and quality of agricultural and horticultural crops.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXltlyqt7k%3D&md5=bafb362aac112d94738dc506d8e7e4ffCAS |
Powlson D (1993) Understanding the soil nitrogen cycle. Soil Use and Management 9, 86–93.
| Understanding the soil nitrogen cycle.Crossref | GoogleScholarGoogle Scholar |
Prasad R, Power J (1995) Nitrification inhibitors for agriculture, health, and the environment. Advances in Agronomy 54, 233–281.
| Nitrification inhibitors for agriculture, health, and the environment.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXlvFCrsb0%3D&md5=40f42aa8d002a70f05356a8ffd5f652bCAS |
Rayment GE, Lyons DJ (2011) ‘Soil chemical methods – Australasia.’ (CSIRO Publishing: Melbourne)
Recous S, Machet J, Mary B (1988) The fate of labelled 15N urea and ammonium nitrate applied to a winter wheat crop: II. Plant uptake and N efficiency. Plant and Soil 112, 215–224.
| The fate of labelled 15N urea and ammonium nitrate applied to a winter wheat crop: II. Plant uptake and N efficiency.Crossref | GoogleScholarGoogle Scholar |
Rotthauwe JH, Witzel KP, Liesack W (1997) The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Applied and Environmental Microbiology 63, 4704–4712.
Ruser R, Schulz R (2015) The effect of nitrification inhibitors on the nitrous oxide (N2O) release from agricultural soils – a review. Journal of Plant Nutrition and Soil Science 178, 171–188.
| The effect of nitrification inhibitors on the nitrous oxide (N2O) release from agricultural soils – a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXkvVahsrs%3D&md5=f0ad4ead1942107e1f9d32b8156d6654CAS |
Searle PL (1984) The Berthelot or indophenol reaction and its use in the analytical chemistry of nitrogen. A review. Analyst (London) 109, 549–568.
| The Berthelot or indophenol reaction and its use in the analytical chemistry of nitrogen. A review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXlsVartbk%3D&md5=d7a93d0d133c8567acf4e496090c6b3aCAS |
Shen J-p, Zhang L-m, Zhu Y-g, Zhang J-b, He J-z (2008) Abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea communities of an alkaline sandy loam. Environmental Microbiology 10, 1601–1611.
| Abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea communities of an alkaline sandy loam.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXnvVWkurk%3D&md5=798b9facecc7e68dd8d320250abf9a8cCAS | 18336563PubMed |
Singh S, Verma A (2007) The potential of nitrification inhibitors to manage the pollution effect of nitrogen fertilizers in agricultural and other soils: a review. Environmental Practice 9, 266–279.
| The potential of nitrification inhibitors to manage the pollution effect of nitrogen fertilizers in agricultural and other soils: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXktFyit74%3D&md5=ffba0dbd32bac639bea8c074bb3d8818CAS |
Stephen JR, Chang YJ, Macnaughton SJ, Kowalchuk GA, Leung KT, Flemming CA, White DC (1999) Effect of toxic metals on indigenous soil β-subgroup proteobacterium ammonia oxidizer community structure and protection against toxicity by inoculated metal-resistant bacteria. Applied and Environmental Microbiology 65, 95–101.
Tomaso JMD (1995) Approaches for improving crop competitiveness through the manipulation of fertilization strategies. Weed Science 43, 491–497.
Weiske A, Benckiser G, Herbert T, Ottow J (2001a) Influence of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) in comparison to dicyandiamide (DCD) on nitrous oxide emissions, carbon dioxide fluxes and methane oxidation during 3 years of repeated application in field experiments. Biology and Fertility of Soils 34, 109–117.
| Influence of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) in comparison to dicyandiamide (DCD) on nitrous oxide emissions, carbon dioxide fluxes and methane oxidation during 3 years of repeated application in field experiments.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXltlyqtL4%3D&md5=b2d586c1d498dafda647d5eaea7be43cCAS |
Weiske A, Benckiser G, Ottow JC (2001b) Effect of the new nitrification inhibitor DMPP in comparison to DCD on nitrous oxide (N2O) emissions and methane (CH4) oxidation during 3 years of repeated applications in field experiments. Nutrient Cycling in Agroecosystems 60, 57–64.
| Effect of the new nitrification inhibitor DMPP in comparison to DCD on nitrous oxide (N2O) emissions and methane (CH4) oxidation during 3 years of repeated applications in field experiments.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xkt1Sitw%3D%3D&md5=a92d5213be9945db80a188cff47c99f7CAS |
Xu C, Wu L-H, Ju X-T, Zhang F-S (2005) Role of nitrification inhibitor DMPP (3,4-dimethylpyrazole phosphate) in NO3 –-N accumulation in greengrocery (Brassica campestris L. ssp. Chinensis) and vegetable soil. Journal of Environmental Sciences (China) 17, 81–83.
Zerulla W, Barth T, Dressel J, Erhardt K, von Locquenghien KH, Pasda G, Rädle M, Wissemeier A (2001) 3,4-Dimethylpyrazole phosphate (DMPP) – a new nitrification inhibitor for agriculture and horticulture. Biology and Fertility of Soils 34, 79–84.
| 3,4-Dimethylpyrazole phosphate (DMPP) – a new nitrification inhibitor for agriculture and horticulture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXltlyqt7g%3D&md5=a15ee234ef5ce9013521b5fc5b89aeccCAS |
Zhang L-M, Hu H-W, Shen J-P, He J-Z (2012) Ammonia-oxidizing archaea have more important role than ammonia-oxidizing bacteria in ammonia oxidation of strongly acidic soils. The ISME Journal 6, 1032–1045.
| Ammonia-oxidizing archaea have more important role than ammonia-oxidizing bacteria in ammonia oxidation of strongly acidic soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XlvVGktrw%3D&md5=5c56c25fa998db06161462d9d4926442CAS | 22134644PubMed |
