
%0 Journal Article
%A Di, H. J. 
%A Cameron, K. C. 
%A McLaren, R. G. 
%T Isotopic dilution methods to determine the gross transformation rates of nitrogen, phosphorus, and sulfur in soil: a review of the theory, methodologies, and limitations
%J Soil Research
%D 2000/01/28
%V 38
%N 1
%P 213-230
%K nutrients, transformations, cycling, tracers, mineralisation, immobilisation immobilisation.
%R https://doi.org/10.1071/SR99005
%U https://www.publish.csiro.au/paper/SR99005 
%X The rates at which nutrients are released to, and removed from, the mineral nutrient pool are important in regulating the nutrient supply to plants. These nutrient transformation rates need to be taken into account when developing nutrient management strategies for economical and sustainable production. A method that is gaining popularity for determining the gross transformation rates of nutrients in the soil is the isotopic dilution technique. The technique involves labelling a soil mineral nutrient pool, e.g. NH4+, NO3&#8722;, PO43&#8722;, or SO42&#8722;, and monitoring the changes with time of the size of the labelled nutrient pool and the excess tracer abundance (atom%, if stable isotope tracer is used) or specific activity (if radioisotope is used) in the nutrient pool. Because of the complexity of the concepts and procedures involved, the method has sometimes been used incorrectly, and results misinterpreted. This paper discusses the isotopic dilution technique, including the theoretical background, the methodologies to determine the gross flux rates of nitrogen, phosphorus, and sulfur, and the limitations of the technique. The assumptions, conceptual models, experimental procedures, and compounding factors are discussed. Possible effects on the results by factors such as the uniformity of tracer distribution in the soil, changes in soil moisture content, substrate concentration, and aeration status, and duration of the experiment are also discussed. The influx and out-flux transformation rates derived from this technique are often contributed by several processes simultaneously, and thus cannot always be attributed to a particular nutrient transformation process. Despite the various constraints or possible compounding factors, the technique is a valuable tool that can provide important quantitative information on nutrient dynamics in the soil&#8211;plant system. 
