Colpomenia species from south and south-eastern Australia (Ectocarpales, Phaeophyceae): a DNA barcoding approach
Nuno Tavares Martins
A E , Carlos Frederico D. Gurgel B C , Tracey M. Spokes C D and Valéria Cassano A
+ Author Affiliations
- Author Affiliations
A Universidade de São Paulo, Instituto de Biociências, Departamento de Botânica, São Paulo, SP 05508-090, Brazil.
B Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Departamento de Botânica, Florianópolis, SC 88040-900, Brazil.
C Department of Environment and Water, State Herbarium of South Australia, Adelaide, SA 5001, Australia.
D The University of Adelaide, School of Biological Sciences, Adelaide, SA 5000, Australia.
E Corresponding author. Email: tavaresmartins.nuno@gmail.com
Australian Systematic Botany 34(6) 587-594 https://doi.org/10.1071/SB21021
Submitted: 29 May 2021 Accepted: 22 September 2021 Published: 14 October 2021
Abstract
Defining species in the brown algal genus Colpomenia is a challenging endeavour because of their morphological similarity, overlapping phenotypic variation, the absence of conspicuous diagnostic characters, and often lack of reproductive structures crucial for their identification. Thus, the use of molecular tools has become widely used to study Colpomenia taxonomy and evolution. The following four Colpomenia species are described along the Australian coast: C. claytoniae M.Boo, K.M.Lee, G.Y.Cho & W.Nelson, C. ecuticulata M.J.Parsons, C. peregrina Sauvageau, and C. sinuosa (Mertens ex Roth) Derbès & Solier. The objective of this study was to assess the diversity of Colpomenia species in southern and south-eastern Australia by using DNA barcoding techniques and single-marker species delimitation methods. We generated 44 new COI-5P DNA sequences from nine different populations across three Australian states (South Australia, Victoria and Tasmania), and applied 13 variations of four species delimitation methods (ABDG, SPN, PTP, GMYC). Our results recognised three Colpomenia species in the region, namely, C. sinuosa, C. claytoniae, and C. peregrina. Colpomenia sinuosa is the most widely distributed species in Australia. Colpomenia peregrina and C. claytoniae presented high levels of intraspecific genetic divergence. We did not find C. ecuticulata, although it has been previously reported from nearby our sampling area.
Keywords: Australia, DNA barcode, COI-5P, Colpomenia, cox1, macroalgae, Phaeophyceae, species delimitation, seaweed.
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