Effect of crude aqueous extract of B. variegata upon growth parameters of Chlorella vulgaris Beijer

Abstract

B. variegata (Ceasalpinaceae) is a woody perennial tree grow in Egypt. Many natural products chemical compounds were isolated from that plant as Kaempferol-3-O-hamnosylglycoside, Malvidin-3-diglucoside, Naringenin, (S)-form, 5,7-dimethylether, 4-glucopyranosyl (1-4)-b-D-glucopyranoside and Peondin-3-O-diglucoside. The effect of crude aqueous extract of B. variegata (2, 4, 6, 8 & 10 ppm) upon growth parameters (cell number, dry weight, total pigments, oxygen evolution and oxygen uptake) of Chlorella vulgaris Beijer cultures for 7 days were studied. Both cell number and total pigments raised gradually with the effect of the concentrations of 2,4 and 6 ppm of crude aqueous extract. The maximum value occurred at 6 ppm of crude aqueous extract. Under the higher concentrations of the aqueous crude extract (8 and 10 ppm) all growth criteria were inhibited. On the other hand, the dry weight, oxygen evolution and oxygen uptake increased to the concentration of 4 ppm of the crude aqueous extract. Under relatively higher concentrations of crude aqueous extract (6,8 & 10 ppm) the dry weight, oxygen evolution and oxygen uptake were sharply inhibited.