Gene cloning and characterization of salt-inducible aldehyde oxidase in barley

Abstract

To survive under environmental stresses, plant developed signal transduction systems. It is important how quickly and efficiently plants sense outer stimuli. However the information involved in signal transductions under salt stress is still limited. In this work we isolated the candidates of salt-stress responsive genes related to signal transductions in barley by differential display. About 30 candidates belong to protein kinase family (MAP kinase, PIP5 kinase, casein kinase, putative serine/ threonine protein kinase, receptor protein kinase, etc.), protein phosphatase and transcription factors (putative SET-domain transcriptional regulator). Among them, one candidate has homology to human EPS15. EPS15 has an EH-domain and interacts with EPS receptor in animal cell. EPS receptor catches certain ligands from out of the cell and then transduces the signal into inner membrane. The interactions between EPS15 and EPS receptor mediated by tyrosine phospholyration are implicated in endocytosis and synaptic vesicle recycling. This is to our knowledge the first report of the relationship between plant salt stress and endocytosis.