
%0 Journal Article
%A TAKABE, Teruhiro 
%A HIBINO, Takashi 
%A ONO, Kiyomi 
%A Tanaka, Yoshito 
%A Suzuki, Shigetoshi 
%A Takabe, Tetsuko 
%T Abiotic tolerance of tobacco plants transformed with DnaK from a halotolerant cyanobacterium Aphanothece halophytica
%J Science Access
%D 2001/01/01
%V 3
%N 1
%P -
%K 
%R https://doi.org/10.1071/SA0403667
%U https://www.publish.csiro.au/paper/SA0403667 
%X A. halophytica can grow at high external salinity up to 3 M NaCl. The DnaK1 from A. halophytica was shown to exhibit extremely high protein folding activity at high salinity conditions. To test the role of the heat shock protein DnaK/Hsp70 in salt tolerance, tobacco plants were transformed with DnaK1 from a halotolerant cyanobacterium A halophytica which was overexpressed in the cytosol. The growth rate and photosynthetic activities of the transgenic and control tobacco plants were similar under non-stressed conditions. After 3 days of treatment with 0.6 M NaCl, the CO2 fixation rate decreased to 40% of that in the non-stressed plants whereas its activity in the transgenic plants was about 85% of that in the non-stressed plants. When the control and transgenic tobacco seeds were incubated at 27°C, more than 95% seeds germinated in both the control and transgenic tobacco. However, at a high incubation temperature, 40°C, only the 27% control seeds germinated whereas the 82% transgenic seeds germinated. These data indicate that the expression of A halophytica DnaK in tobacco confers the tolerance of tobacco for salt- and high temperature-stresses. 
