
TY  - JOUR
A1  - Mangels, Dorothea 
A1  - Kruip, Jochen 
A1  - Berry, Stephan 
A1  - Roegner, Matthias 
A1  - Boekema, Egbert J 
A1  - Koenig, Friederikek 
T1  - Purification and characterisation of photosystem I from the unusual cyanobacterium
Gloeobacter violaceus
JF  - Science Access
JO  - 
Y1  - 2001
VL  - 3
IS  - 1
SP  - 
EP  - 
KW  - 
UR  - https://doi.org/10.1071/SA0403624 
N2  - Photosystem I from the highly unusual primitive cyanobacterium Gloeobacter violaceus was purified. The isolated Photosystem I complexes have the same subunit composition as complexes from other cyanobacteria as determined by high resolution gel electrophoresis and immunoblotting. They exist both in a trimeric and in a monomeric form as shown by gel filtration and electron microscopy. Image analysis of single particles shows that the size and shape of the particles closely resembles other cyanobacterial Photosystem I particles. Flash induced absorption difference spectra show a classical P700 shape with kinetics and relaxation times similar to Photosystem I from Synechocystis, but its amplitude indicates a much bigger antenna size, resembling the spectra of higher plants rather than those of other cyanobacteria. 77 K fluorescence emission spectra lack the typical long wavelength emission peaks of cyanobacterial Photosystem I. In addition, the preparation contains novel polypeptides not related to known Photosystem I subunits. Immunoblotting shows the possible existence of a membrane intrinsic light-harvesting protein related to LHC proteins of Chromophytes. Moreover, electron microscopy reveals a novel T-shaped complex that has not been observed so far. 
ER  -

