
%0 Journal Article
%A Seo, Daisuke 
%A TOMIOKA, Atusi 
%A KUSUMOTO, Noriaki 
%A Kamo, Masaharu 
%A Enami, Isao 
%A Sakurai, Hidehiro 
%T Reduction of ferredoxins by photosynthetic reaction center complex from the green sulfur bacterium Chlorobium tepidum
%J Science Access
%D 2001/01/01
%V 3
%N 1
%P -
%K 
%R https://doi.org/10.1071/SA0403303
%U https://www.publish.csiro.au/paper/SA0403303 
%X Green sulfur bacterial reaction centers (RCs) belong to an iron-sulfur type RC, and can photoreduce low potential iron-sulfur protein, ferredoxin, as does PSI. From the thermofilic green sulfur bacterium, C. tepidum, four 2[4Fe-4S] type Fd fractions were purified. The amount of each Fd fractions varied depending upon culture conditions. Absorbances at 385nm of these Fds decreased by 10-15% in 6 days at 4oC in aerobic condition, indicating that these Fds were rather stable in contrast with previous reports. Two of these Fds¿ N-terminal amino acid sequences (15 residues) were completely identical. All of these Fds¿ N-terminal amino acid sequences (15-25 residues) completely agreed to those deduced from the three fdx genes (Chung S., and Bryant D.A.). These Fds are photoreducible by purified RC from this bacterium, and support NADP+ photoreduction with spinach ferredoxin-NADP+ reductase. The Vmax and Km of electron transfer rates in the above reaction were significantly different among Fds. 
