
%0 Journal Article
%A Ihalainen, Janne 
%A Jensen, Poul Erik 
%A Haldrup, Anna 
%A van Stokkum, Ivo 
%A van grondelle, rienk 
%A Scheller, Henrik 
%A dekker, jan 
%T Spectroscopic characterization of isolated PSI-200 complexes from plants devoid of the PSI-G, PSI-K, PSI-L and PSI-N subunits
%J Science Access
%D 2001/01/01
%V 3
%N 1
%P -
%K 
%R https://doi.org/10.1071/SA0403174
%U https://www.publish.csiro.au/paper/SA0403174 
%X We present a spectroscopic analysis of isolated PSI-200 particles from Arabidopsis thaliana and its mutants lacking the PSI-G, PSI-K, PSI-L and PSI-N subunits. Low-temperature absorption and linear dichroism spectra indicate that the PSI-200 particles from wild-type plants and plants lacking PSI-N are identical, suggesting the absence of pigments in PSI-N, whereas the particles lacking PSI-G showed decreased absorption of a carotenoid and those lacking PSI-K showed smaller contributions from the LHCI antenna, most likely Lhca2 and Lhca3. The largest changes were observed in particles without PSI-L, in which several chlorophyll absorption bands were affected. Time-resolved fluorescence measurements at room temperature, detected with a synchroscan streak camera, revealed excitation equilibration lifetimes of about 4 and 20 ps and excitation trapping lifetimes of about 45 and 115 ps in all particles. The largest differences among the mutants were observed in a long-living component caused by unconnected molecules, which was less pronounced in the PSI-L less particles. 
