%0 Journal Article %A Ji, Ben-Hua %A Ji, Ben-Hua %A Jiao, Demao (D-M) %A Ji, Ben-Hua %T Photochemical efficiency of PS_and membrane lipid peroxidation in leaves of indica and japonica rice (Oryza sativa L.) under different temperates and light intensities %J Science Access %D 2001/01/01 %V 3 %N 1 %P - %K %R https://doi.org/10.1071/SA0403082 %U https://www.publish.csiro.au/paper/SA0403082 %X Potted indica and japonica rice plants were put in phytotron with day/night temperature of 26±1ºC/22±1ºC and 11±1ºC/l0±1ºC, and the RH of 78-82%. The photoperiod was 12 h with the PFD of 350, 700, 1050 and 1400 µmol m-2 s-1, respectively, from 6:00 to 18:00 for 4 days. Xanthophyll cycle components were assayed by HPLC system. Measurements of chlorophyll fluorescence, 14C-Atrazine-bound DI protein, SOD activity, O2? forming rate and membrane lipid peroxidation product (MDA) were carried out. Results were as follows: (1) No changes in PSII photochemical efficiency (Fv/Fm) and MDA were observed, so neither photoinhibition nor photooxidation happened in indica and japonica rice under moderate temperature and medium PFD; (2) Fv/Fm dropped obviously with no change in MDA contents, and photoinhibition appeared in indica rice either under medium temperature and higher PFD or under lower temperature and medium PFD; (3) Dl protein, Fv/Fm (A+Z)/(A+Z+V), qN, and SOD activities dropped, and contents of O2? and MDA increased accordingly, and both photoinhibition and photooxidation appeared in indica and japonica rice under lower temperature and higher PD; (4) Experiment with inhibitors (SM, DTT and DDTC) under lower temperature and higher PFD showed that indica rice had a decrease in Dl protein contents, SOD activities, an inhibition in xanthophyll cycle and qN within a greater range, and a higher level of MDA, thus indica rice exhibited more distinct photoinihibition and photooxidation as compared with japonica rice. We suggested that Fv/Fm and membrane lipid peroxidation product (MDA) were the key indexes to predict and diagnose photooxidaton.