
TY  - JOUR
A1  - Nakahara, M 
A1  - Kitashima, M 
A1  - Ohtake, H 
A1  - Inoue, K 
T1  - Investigation of bacteriochlorophyll biosynthesis genes in Blastochloris viridis by functional complementation of mutants of Rhodobacter capsulatus
JF  - Science Access
JO  - 
Y1  - 2001
VL  - 3
IS  - 1
SP  - 
EP  - 
KW  - 
UR  - https://doi.org/10.1071/SA0403052 
N2  - Blastochloris (formerly Rhodopseudomonas) viridis produces bacteriochlorophyll (BChl) b which is distinguished from BChl a by the possession of an ethylidene group at C8 instead of an ethyl. This chemical structure enables to BChl b to absorb longer wave length light (~1100 nm) than BChl a. To investigate genes involved in the BChl b biosynthesis, we have constructed genomic DNA libraries from B. viridis and introduced them to mutants of the nonsulfur purple bacterium Rhodobacter capsulatus which is deficient in BChl a biosynthesis.  Total ten kinds of R. capsulatus mutants, three Mg-chelatase (BchD, H, I) mutants, three protochlorophyllide reductase (BchB, L, N) mutants, Mg-protoporphyrin IX methyltransferase (BchM) mutant, Mg-protoporphyrin IX monomethylester oxidative cyclase (BchE) mutant, bacteriochlorophyll synthase (Bch G) mutant, and 2-vinyl-bacteriochlorophyllide hydratase (BchF) mutant, were functionally complemented by B. viridis library DNA. The complemented R. capsulatus strains were demonstrated to be capable of synthesizing BChl a under photoautotrophic growth conditions. 
ER  -

