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RESEARCH ARTICLE
Contents Vol 51(10)

Specific physiological responses to alkaline carbonate stress in rice (Oryza sativa) seedlings: organic acid metabolism and hormone signalling

Dan Wang A B # , Miao Xu B # , Teng-yuan Xu A , Xiu-yun Lin C , Elshan Musazade B , Jing-mei Lu D , Wei-jie Yue A , Li-quan Guo https://orcid.org/0000-0003-3424-2238 B * and Yu Zhang E *
+ Author Affiliations
- Author Affiliations

A School of Public Health, Jilin Medical University, Jilin 132013, PR China.

B College of Life Sciences, Jilin Agricultural University, Changchun 130118, PR China.

C Jilin Academy of Agricultural Sciences, Changchun 130118, PR China.

D School of Life Sciences, Jilin University, Changchun 130062, PR China.

E Land Requisition Affairs Center of Jilin Province, Changchun 130061, PR China.

* Correspondence to: guolq948@nenu.edu.cn, zhangyu0062022@163.com

Handling Editor: Fanrong Zeng

Functional Plant Biology 51, FP23161 https://doi.org/10.1071/FP23161
Submitted: 28 July 2023  Accepted: 28 August 2024  Published: 19 September 2024

© 2024 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

In recent years, alkaline soda soil has stimulated numerous biological research on plants under carbonate stress. Here, we explored the difference in physiological regulation of rice seedlings between saline (NaCl) and alkaline carbonate (NaHCO3 and Na2CO3) stress. The rice seedlings were treated with 40 mM NaCl, 40 mM NaHCO3 and 20 mM Na2CO3 for 2 h, 12 h, 24 h and 36 h, their physiological characteristics were determined, and organic acid biosynthesis and metabolism and hormone signalling were identified by transcriptome analysis. The results showed that alkaline stress caused greater damage to their photosynthetic and antioxidant systems and led to greater accumulation of organic acid, membrane damage, proline and soluble sugar but a decreased jasmonic acid content compared with NaCl stress. Jasmonate ZIM-Domain (JAZ), the probable indole-3-acetic acid-amido synthetase GH3s, and the protein phosphatase type 2Cs that related to the hormone signalling pathway especially changed under Na2CO3 stress. Further, the organic acid biosynthesis and metabolism process in rice seedlings were modified by both Na2CO3 and NaHCO3 stresses through the glycolate/glyoxylate and pyruvate metabolism pathways. Collectively, this study provides valuable evidence on carbonate-responsive genes and insights into the different molecular mechanisms of saline and alkaline stresses.

Keywords: abscisic acid (ABA) signalling, alkaline stress, auxin signalling, jasmonic acid (JA) signalling, organic acid biosynthesis, rice, transcriptome analysis, UDP-glycosyltransferase.

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