Transcriptomic study of Suaeda salsa in response to salt and drought stress
Zhijie Ding
A # , Zhiyou Liu B # , Jinbo Bao A C , Yuwei Wang A C , Jialei Li A C , Qiuyan Wang A * and Xinmin Tian A C *
A Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, College of Life Science, Guangxi Normal University, Ministry of Education,
B City Management and Service Centre of Tiemenguan,
C Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University,
Handling Editor: Heng Zhang
Functional Plant Biology 50(10) 765-776 https://doi.org/10.1071/FP23050
Submitted: 25 February 2023 Accepted: 21 July 2023 Published: 11 August 2023
Abstract
Drought and salinity are the main factors limiting agricultural production. Improving crop resistance to relieve land stress is a major challenge in agriculture. The salt-tolerant species Suaeda salsa is a typical indicator of saline soil. It has a strong drought tolerance and can be used as a model plant to study salt and drought tolerance in plants. In this study, transcriptome sequencing and bioinformatic analysis were performed to study gene expression changes in S. salsa under salt and drought stresses, and to screen out differentially expressed genes. The genetic changes were most abundant in cellular processes, metabolic processes, ion binding, signalling, post-translational modifications, protein conversion, and molecular chaperones, suggesting that the above methods may play a significant role in the response of S. salsa to external salt and drought stress. Enrichment analysis showed that carbohydrate metabolic processes, oxidoreductase activity, transmembrane transport, kinase activity, cellular protein modification processes, and ion-binding pathways are involved in the stress response of S. salsa.
Keywords: differentially expressed genes, drought stress, RNA-seq, salt stress, signalling pathway, stress tolerance mechanism, Suaeda salsa, transcriptome.
References
Almashhadani AQ, Leh CP, Chan SY, Lee CY, Goh CF (2011) Nanocrystalline cellulose isolation via acid hydrolysis from non-woody biomass: importance of hydrolysis parameters. Carbohydrate Polymers 286, 119285.
| Crossref | Google Scholar |
Cui K, Wu WW, Diao QY (2019) Advances in research and application of transcriptome sequencing technology. Biotechnology Bulletin 35, 1-9.
| Crossref | Google Scholar |
Eva VZ, Zhang YX, Christa T (2020) Salt tolerance mechanisms of plants. Annual Review of Plant Biology 71, 403-433.
| Crossref | Google Scholar |
Flowers TJ, Colmer TD (2008) Salinity tolerance in halophytes. New Phytologist 179, 945-963 PMID:.
| Crossref | Google Scholar | PubMed |
Fu C, Huang Y (2011) Transcriptomics platform technology and its application in the molecular biology of plant stress resistance. Biotechnology Bulletin 6, 40-46.
| Google Scholar |
Fu C, Sun YG, Fu GR (2013) Advances in the molecular mechanisms of salt tolerance in saline plants. Biotechnology Bulletin 1, 1-7.
| Google Scholar |
Gong JP, Zhang Z, Tian CY, Wang L, Zhao ZY (2019) An introduction to the Suaeda salsa, a salt plant of Xinjiang. Xinjiang Agricultural Reclamation Science and Technology 42, 15-16.
| Google Scholar |
Hernández JA, Ferrer MA, Jiménez A, Barceló AR, Sevilla F (2001) Antioxidant systems and O2.-/H2O2 production in the apoplast of pea leaves. Its relation with salt-induced necrotic lesions in minor veins. Plant Physiology 127, 817-831 PMID:.
| Crossref | Google Scholar | PubMed |
Hu H, Liu H, Liu F (2018) Seed germination of hemp (Cannabis sativa L.) cultivars responds differently to the stress of salt type and concentration. Industrial Crops and Products 123, 254-261.
| Crossref | Google Scholar |
Huo D, Zhang H, Dai SJ (2011) Transcriptomic analysis of saline stress response in saline plants. Modern Agricultural Science and Technology 5, 11-12.
| Google Scholar |
Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, Katayama T, Kawashima S, Okuda S, Tokimatsu T, Yamanishi Y (2007) KEGG for linking genomes to life and the environment. Nucleic Acids Research 36, D480-D484 PMID:.
| Crossref | Google Scholar | PubMed |
Kovda VA (1983) Loss of productive land due to sailnazation. Ambio 2, 91-93.
| Google Scholar |
Liang Y, Chen SY, Liu GS (2011) Application of next-generation sequencing technologies in plant transcriptome research. Genetics 33, 1317-1326 PMID:.
| Crossref | Google Scholar | PubMed |
Liu W, Guo GY, Mi CL (2019) An overview of the main research techniques in transcriptomics and their applications. Teaching Biology 44, 2-5.
| Google Scholar |
Park HJ, Kim WY, Yun DJ (2013) A role for GIGANTEA: keeping the balance between flowering and salinity stress tolerance. Plant Signaling & Behavior 8, e24280.
| Crossref | Google Scholar |
Valliyodan B, Nguyen HT (2006) Understanding regulatory networks and engineering for enhanced drought tolerance in plants. Current Opinion in Plant Biology 9, 189-195 PMID:.
| Crossref | Google Scholar | PubMed |
Wang X, Wang XW, Wang LK, Feng ZX (2010) Processing and analysis of next-generation high-throughput RNA sequencing data. Advances in Biochemistry and Biophysics 37, 834-846.
| Google Scholar |
Wang XN, Wei YY, Zhou W, Li L (2022) Effects of salicylic acid on Cucumis sativus L. seed germination and seedling root growth under salt stress. Molecular Plant Breeding 20, 524-528.
| Google Scholar |
Xi JB, Zhang FS, Mao DR (2005) Distribution of saline soils and saline plant resources in Xinjiang. Soil Bulletin 36, 299-303.
| Google Scholar |
Yang Z, Wang BS (2015) Present situation of saline soil resources in China and countermeasures for improvement and utilization. Shandong Agricultural Sciences 47, 125-130.
| Google Scholar |
Young MD, Wakefield MJ, Smyth GK, Oshlack A (2010) Gene ontology analysis for RNA-seq: accounting for selection bias. Genome Biology 11, R14.
| Crossref | Google Scholar |
Zhang CL, Qin ZJ, Wang GZ (2012) Transcriptome and RNA-Seq technology. Biotechnology Bulletin 12, 51-56.
| Google Scholar |
Zhao KF, Fan H, Jiang XY (2002) The role of saline plants in the improvement of saline soils. Journal of Applied and Environmental Biology 8, 31-35.
| Google Scholar |
