Combined physiological responses and differential expression of drought-responsive genes preliminarily explain the drought resistance mechanism of Lotus corniculatus
Leiting Wang
A , Zhongling Jian A , Puchang Wang B , Lili Zhao A C * and Keke Chen A
+ Author Affiliations
- Author Affiliations
A College of Animal Science, Guizhou University, Guiyang 550025, China.
B Guizhou Institute of Prataculture, Guiyang 550006, China.
C State Engineering Technology Institute for Karst Rocky Desertification Control, Guiyang 550025, China.
Functional Plant Biology 50(1) 46-57 https://doi.org/10.1071/FP22051
Submitted: 28 February 2022 Accepted: 5 August 2022 Published: 29 August 2022
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Lotus corniculatus L. is a perennial high-quality legume forage species but is vulnerable to drought, and water deficit reduces productivity. To understand the drought response mechanism of L. corniculatus, we investigated physiological responses under drought stress and constructed suppression subtractive hybridisation (SSH) cDNA libraries to isolate drought-inducible genes and quantitatively study the expression levels of candidate drought- responsive genes. Genes encoding calmodulin-like protein, mitogen-activated protein kinase, indole-3-acetic acid-induced protein, ser/thr-protein phosphatase homolog-related proteins, and β-galactosidase-related protein with hydrolysis activity were isolated and considered the main factors that explained the resistance of L. corniculatus to drought. Approximately 632 expressed sequence tags (ESTs) were identified and confirmed in the constructed SSH library. The Gene Ontology (GO) analysis revealed that these genes were involved mainly in transcription processes, protein synthesis, material metabolism, catalytic reactions, sugar metabolism, and photosynthesis. The interaction between the functions of these drought-related genes and the physiological responses preliminarily explains the drought resistance mechanisms of L. corniculatus.
Keywords: adaptive strategy, drought-resistant mechanism, drought stress, gene expression, legume forage, Lotus corniculatus, physiological response, suppression subtractive hybridisation (SSH).
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