A Panax notoginseng phosphate transporter, PnPht1;3, greatly contributes to phosphate and arsenate uptake
Guan-hua Cao A # , Xi-fu Wang A # , Ze-dong Li A , Xue Zhang A , Xiao-gang Li A , Wen Gu A , Fan Zhang A , Jie Yu A * and Sen He
A B *
+ Author Affiliations
- Author Affiliations
A School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicine Utilization, Yunnan University of Chinese Medicine, Kunming, Yunnan 650500, China.
B Yunnan Key Laboratory for Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming, Yunnan 650500, China.
* Correspondence to: cz.yujie@gmail.com, senhe2019@outlook.com
# These authors contributed equally to this paper
Handling Editor: Tim Cavagnaro
Functional Plant Biology 49(3) 259-271 https://doi.org/10.1071/FP21218
Submitted: 27 January 2021 Accepted: 17 December 2021 Published: 4 February 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)
Abstract
The crisis of arsenic (As) accumulation in rhizomes threatens the quality and safety of Panax notoginseng(Burk.) F.H. Chen, which is a well-known traditional Chinese herb with a long clinical history. The uptake of arsenate (AsV) could be suppressed by supplying phosphate (Pi), in which Pi transporters play important roles in the uptake of Pi and AsV. Herein, the P. notoginseng Pi transporter-encoding gene PnPht1;3 was identified and characterised under Pi deficiency and AsV exposure. In this study, the open reading frame (ORF) of PnPht1;3 was cloned according to RNA-seq and encoded 545 amino acids. The relative expression levels revealed that PnPht1;3 was significantly upregulated under phosphate deficiency and AsV exposure. Heterologous expression in Saccharomyces cerevisiae MB192 demonstrated that PnPht1;3 performed optimally in complementing the yeast Pi-transport defect and accumulated more As in the cells. Combined with the subcellular localisation prediction, it was concluded that PnPht1;3 encodes a functional plasma membrane-localised transporter protein that mediates putative high-affinity Pi/H+ symport activity and enhances the uptake of Pi and AsV. Therefore, a better understanding of the roles of the P. notoginseng Pi transporter could provide new insight for solving As accumulation in medicinal plants.
Keywords: arsenate (AsV) exposure, arsenic (As) acquisition and accumulation, heterologous expression, molecular mechanism, Panax notoginseng, phosphate (Pi) transporter, Pi deficiency, relative expression level.
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